Team:ULB-Brussels/Modelling

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    Université Libre de Bruxelles  **/                -->
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Abstract</p></td></tr>
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<a href="https://2014.igem.org/Team:ULB-Brussels/Modelling/Population-Dynamics"><b> Pop Dyn ></b></a>
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<a href="https://2014.igem.org/Team:ULB-Brussels/Modelling/TA-System"><b> TA Sys ></b></a>
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<a href="https://2014.igem.org/Team:ULB-Brussels/Modelling/2A-Peptid"><b> 2A Pep ></b></a>
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<font color="#CCD6EA">'Now begins an</font> $Overview$ <font color="#CCD6EA">about Modelling'</font>
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<section style="text-align: justify; margin: 50px"><p>In this page, the modelling part will be detailed with previews about our biological system and the results with numerical simulations of bacteria populations as well as structural components of the chosen plasmids.</p>
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<p>Afterwards, these simulations will be compared with experimental results. In parallel, an estimation of the production in sub-populations cells in bioreactors by coupling the recombinant protein with an essential protein and a numerical estimation will be purposed. Now, we're beginning to perform it.</p>
 
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<h1>Population Dynamics Model</h1>
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<a href="https://2014.igem.org/Team:ULB-Brussels/Modelling/Population-Dynamics"><b> Population Dynamics > </b></a>
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<p>The growth of bacteria involves ...</p>
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<h3>By Probabilities</h3>
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When some new plasmids are introduced into the cytoplasm of E.Coli, These plasmids can be lost after cell division or replication, so it's interesting to study a model based on the different possibilities of plasmid combinations in bacteria, like in the studies of mutations in animals. A typical example of similar is to study the mutations of the color of the eyes in a family, by vertical transfer of genes
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<it>(A vérifier si malgré que les bactéries se divisent, il ne s'agit pas de transfert horizontal, c'est le transfert horizontal qui a lieu si l'Hypothèse de Persistence est valable)</it>. </p>
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A Probabilistic Model is easy to but necessits some assumptions
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<it>(+ Voir suite sur la partie de Denis, pour bientôt)</it>. </p>
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<h3>By Logistic Equation</h3>
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The Logistic Equation was initially introduced during the beginning of the XIXth Century, by the belgian mathematician P.F. Verhulst. Now, this equation is mainly used in Population Dynamics Models, especially in Biological Sciences.
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<h1>Toxin-Antitoxin Systems</h1>
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<p>Two type II TA systems have been planned to be investigated in our project.</p>
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The first consist of ccdB (the toxin, T) and ccdA (the antitoxin, A) and for the second, these are Kid (T) and Kis (A).</p>
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<h3>CcdBA</h3>
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<h3>Kis/Kid</h3>
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-Taux de clivage du F2A et réplication sous l'influence de l'ADN gyrase |
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-Rôle de la proline |
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-Milieu avec glucose(AraC est répresseur) / arabinose(AraC est inducteur) |
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-Critères de sélection pour l'antibiotique |
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-Biorécteur |
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Latest revision as of 22:57, 17 October 2014

$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$ Example of a hierarchical menu in CSS

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'Now begins an $Overview$ about Modelling'


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