From 2014.igem.org
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PCR |
- Amplified mCherry gene with negative control group and positive control group
- Reaction Mixture (a master mix to be divided into 3 tubes; a. Mq water 10.2 uL X 4 = 40.8 uL, b. 2x Phusion polymerase HF buffer mix 12.5 uL x 4 = 50 uL, c. Gene 0.5 uL x 4 = 2 uL, d. Forward primer 0.9 uL X 4= 3.6 uL, e. Reverse primer 0.9 uL x 4 = 3.6 uL
- Final Reaction Mixture = 96.4 uL with 24.1 uL in each tube
- Annealing temperature = 65 Celsius
- Run products on a gel for verification (2.5 uL of each)
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