Team:UFAM Brazil/Background

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<p>In the Amazon, mercury is considered one of the region main contaminants because is utilized in mines, being part of the <a href="#" class="tooltip"> gold amalgamation <span> This operation consists of the connection of mercury with another metal, or the separation of gold and ganga's silver, through mercury.</span></a> (Au-Hg) process. Where a quantity of metallic mercury (Hg0) is released upon rivers and soils by the <a href="https://2014.igem.org/Team:UFAM_Brazil/Safety" target="_blank">handling  in insecure situations </a>, and through the outdoor burning process, where there is a release of the element's vapor to the atmosphere, practices that cause the aquatic ecosystem contamination.</p>
 
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<p>In the Amazon effluents, around 3.000 tons of mercury are found available.  This mercury can be bio-converted into forms that are more toxic to human beings, through de methylation - the addition of a CH3 radical - of mercury, acquiring
 
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bio-accumulative  properties<span>not easily expelled through the living beings, increasing  the concentration in the organisms as they are ingested or inhaled.</span></a>
 
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, due to its affinity to animal tissues (Souza and Barbisa 2010). The mercury methylation is a crucial step to its entry in the aquatic organisms food chain, which are the biggest bio-concentrators of this metal, therefore they contaminate the riverside communities through the fish ingestion.</p>
 
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<p>Taking in consideration that the
 
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<a href="#" class="tooltip">riverside communities<span>a traditional pupulation that resides in the rivers proximities and they have the artisanal fishing as main food supplying activity.</span></a>,
 
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during a long period, have as their major source of proteins, the consumption of fish, thus they became more vunerable to a high Hg concentration in their bodies, causing the following <a href="https://2014.igem.org/Team:UFAM_Brazil/Methylmercury_Poisoning" target="_blank">harmfull effects</a> : abdominal pains, diarrhea, osteoporosis, brain and renal lesions, psychological and psychomotor alterations.</p>
 
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<p>In the environment, the successive transformations suffered by Hg, represent risks not only for superior organisms, but also for microorganisms. However, is known that some bacterias own resistance to mercury, among them <i>Serratia marcescens, Pseudomonas putida, Cupriavidus metallidurans, Entereobacter</i>. The bacteria resistance occurs by the activity of proteins that are expressed through a gene set denominated
 
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<a href="#" class="tooltipImg">operon mer <span style="bottom:-150px; left:80px;"><img src="https://static.igem.org/mediawiki/2014/b/ba/UFAM_Brazil_2014_OperonMER.png" width="400"></span></a>
 
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. This operon has big bio-remediating capacity for presenting the proteins
 
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<a href="#" class="tooltipImg"> merA e merB<span style="right:10%;"><img src="https://static.igem.org/mediawiki/2014/8/8d/UFAM_Brazil_2014_merBreacao.gif" width="700"></span></a>
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<p>Human actions derived from industrial activities, agriculture and domestic sewage are among those responsible for the pollution of the environment by toxic metals (Biondo, 2008). Mercury occurs in the environment combined with other elements and naturally in different chemical species with distinct solubility, reactivity and toxicity. Occurring in volcanoes and mercury mines, which are responsible for emissions of mercury in the order of 2700-6000 tons/year (Micaroni et al. 2000). In the Amazon, it is also used in mines in the amalgamation of gold (Au-Hg) (Figura 1), where a quantity of metallic mercury (Hg0) is released upon rivers and soils by the <a href="https://2014.igem.org/Team:UFAM_Brazil/Safety" target="_blank">handling  in insecure situations</a>, and through the outdoor burning process, which releases element's vapor to the atmosphere, that leads to the aquatic ecosystem contamination.
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which catalyze Hg2+ and CH3Hg, respectively, converting then into Hg0 (volatile in room temperature). In addition to the catalytic function, the operon presents proteins that hold carrier functions
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<p align="center"><img src="https://static.igem.org/mediawiki/2014/1/18/UFAM_BRAZIL_2014_Background.png" width="300"></p>
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<p align="center">Figure 1. Direct contact with mercury in the Amalgamation of gold process.</p>
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<a href="#" class="tooltipImg"> merP e merT<span style="bottom:0px; left:80px;"><img src="https://static.igem.org/mediawiki/2014/a/aa/UFAM_Brazil_2014_merP-merT.png" width="400"></span></a>
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<p>In Amazon rivers, about 3000 tons of mercury are available and can be bioconverted in its most toxic forms for living beings, through methylation - the addition of the radical CH3 – of mercury, bioaccumulative properties are acquired by its high affinity to animal tissues (Souza and Barbosa, 2010). The mercury methylation is a crucial step to its entry in the aquatic food chain organisms, because of it <a href="#" class="tooltip">riverside communities<span>A traditional pupulation that resides in the rivers proximities and they have the artisanal fishing as main food supplying activity.</span></a> are the main affected by contaminated fish ingestion.
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, which carry Hg of the periplasm to the cytoplasm (Biondo 2008).</p>
 
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<p>On the above, UFAM_Brazil Team aims to utilize the operon Mer mechanism to solve the adversities created by the inappropriate use of Hg through the synthetic biology technologies.</p>
 
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Revision as of 15:47, 17 October 2014

Background

Human actions derived from industrial activities, agriculture and domestic sewage are among those responsible for the pollution of the environment by toxic metals (Biondo, 2008). Mercury occurs in the environment combined with other elements and naturally in different chemical species with distinct solubility, reactivity and toxicity. Occurring in volcanoes and mercury mines, which are responsible for emissions of mercury in the order of 2700-6000 tons/year (Micaroni et al. 2000). In the Amazon, it is also used in mines in the amalgamation of gold (Au-Hg) (Figura 1), where a quantity of metallic mercury (Hg0) is released upon rivers and soils by the handling in insecure situations, and through the outdoor burning process, which releases element's vapor to the atmosphere, that leads to the aquatic ecosystem contamination.

Figure 1. Direct contact with mercury in the Amalgamation of gold process.

In Amazon rivers, about 3000 tons of mercury are available and can be bioconverted in its most toxic forms for living beings, through methylation - the addition of the radical CH3 – of mercury, bioaccumulative properties are acquired by its high affinity to animal tissues (Souza and Barbosa, 2010). The mercury methylation is a crucial step to its entry in the aquatic food chain organisms, because of it riverside communitiesA traditional pupulation that resides in the rivers proximities and they have the artisanal fishing as main food supplying activity. are the main affected by contaminated fish ingestion.