Team:UCLA/Project

From 2014.igem.org

(Difference between revisions)
Line 64: Line 64:
repeated over and over are the key to the formation of one of the strongest materials known to man.</p>
repeated over and over are the key to the formation of one of the strongest materials known to man.</p>
     </div>
     </div>
-
    <div class= "content_subsection">
+
<!--SUMMARY TILES-->
-
          <h1> Title </h1>
+
 
-
          <figure style= "margin: 10px; float: right;"><img src= "https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" /><figcaption style="margin: auto;">Fig1: Here is a placeholder.</figcaption></figure>
+
<br/>
-
          <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
+
    <!-- Enter new info -->
-
    </div>
+
 
-
    <div class= "content_subsection">
+
<div id= "centercontainer">
-
          <h1> Title </h1>
+
 
-
          <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
+
<!-- End new info -->
-
          <figure style= "margin: 10px; float: right;"><img src= "https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" /><figcaption style="margin: auto;">Fig1: Here is a placeholder.</figcaption></figure>
+
<div class="boxgrid contentBorder boxgridMedium" id="box5" style="display: inline-block; ">
-
          <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p><br/><br/>
+
  <img src="http://images.medicaldaily.com/sites/medicaldaily.com/files/styles/large/public/2013/11/25/shutterstock-dna-analysis-image.jpg?itok=h5ta9Ajv">
-
    </div>
+
  <a class="cover boxcaption" style="top: 340px;" href="/Team:UCLA/Project/Customizing_Silk">
 +
    <h2 class="onBlack">Customizing Silk</h2>
 +
    <p class="onBlackIntro">
 +
 
 +
Modifying the genetic structure of silk can create a diverse new range of biomaterials.
 +
    </p>
 +
    <p class="onBlack">
 +
With spider silk as our basis, we started to experiment with the genetic structure of silk. We rearranged portions of the repetitive DNA code to see how it affected the strength, elasticity, and spinning capabilities using Golden Gate Cloning and Iterative Capped Assembly. Click this text to learn about this portion of our project in-depth.
 +
    </p>
 +
  </a>
</div>
</div>
 +
<div class="boxgrid contentBorder boxgridMedium" id="box6" style="display: inline-block; ">
 +
  <img src="http://www.wired.com/images_blogs/wiredscience/2013/06/Slide1.jpg">
 +
  <a class="cover boxcaption" style="top: 340px;" href="/Team:UCLA/Project/Functionalizing_Fibers">
 +
    <h2 class="onBlack">Functionalizing Fibers</h2>
 +
    <p class="onBlackIntro">
 +
By attaching various proteins, we can create silk with specific applications.
 +
    </p>
 +
    <p class="onBlack">
 +
Glowing silk is an idea that appeals both to the scientific and artistic worlds. It is a proof of concept for the scientists, demonstrating how we are able to functionalize silk fibers, and an interesting new material to work with for the artists. This method can be applied to different kinds of proteins with different functions as well, but every idea has to start small. To see how we attached GFP to our silk constructs, click this text.
 +
    </p>
 +
  </a>
 +
</div>
 +
<div class="boxgrid contentBorder boxgridMedium" id="box7" style="display: inline-block; ">
 +
  <img src="http://www.seidentraum.biz/WebRoot/Store11/Shops/64114803/51CF/36A1/C6E8/3543/4137/C0A8/29BA/297E/seidenfasern_mb.jpg">
 +
  <a class="cover boxcaption" style="top: 340px;" href="/Team:UCLA/Project/Spinning_Silk">
 +
    <h2 class="onBlack">Spinning Silk</h2>
 +
    <p class="onBlackIntro">
-
<div class= "page_content" id= "section2">
+
Engineering our silk is only half the work, creating a tangible product is the rest.
-
    <div class= "content_subsection">
+
    </p>
-
          <h1> Title </h1>
+
    <p class="onBlack">
-
          <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
+
After some stumbles in the beginning of summer when our makeshift rotary jet spinner failed to work, we decided to look into different methods of spinning synthetic fibers. We tested extrusion with a syringe pump, which gave us very thin, fragile fibers. Click this text to jump into the action and watch fibers take shape from solution.
-
    </div>
+
    </p>
-
    <div class= "content_subsection">
+
  </a>
-
          <h1> Title </h1>
+
</div>
-
          <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
+
-
    </div>
+
     <div class= "content_subsection">
     <div class= "content_subsection">
           <!--main content -->
           <!--main content -->

Revision as of 01:37, 15 October 2014

iGEM UCLA




























Background

The mechanical properties of spider silk are a result of its primary amino acid sequence. Although the amino acid sequences for various types of spider silk are well documented, it is still relatively unknown how these amino acids aggregate and contribute to the strength and stability of silk. Silk proteins are composed of repeats of short amino acid sequences (approximately 33 amino acids). These repeats can be referred to as monomers. Each monomer consists of a glycine rich stretch, followed by an alanine rich stretch. It is hypothesized that these hydrophobic alanines form beta sheet crystals with other silk proteins, and contribute to the strength of the silk fibers. The glycine stretches are thought to form alpha helices and contribute to the flexibility of the protein. This monomer sequence is repeated many times in one protein, and allow for strong interactions with other silk proteins. These simple motifs repeated over and over are the key to the formation of one of the strongest materials known to man.





Project Description

Content

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)


References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.

You can use these subtopics to further explain your project

  1. Overall project summary
  2. Project Details
  3. Materials and Methods
  4. The Experiments
  5. Results
  6. Data analysis
  7. Conclusions

It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.

It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.

Retrieved from "http://2014.igem.org/Team:UCLA/Project"