Team:UCLA/Notebook/Protocols

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iGEM UCLA




Protocols

Equipment for the collection of recombinant silk Equipment Protocol
List of Reagents Reagent Protocol
PCR for MASP1 and MASP2 PCR Protocol
Silk Fibroin Extraction Silk Fibroin Extraction Protocol
Silk Films Silk Films Protocol
Electrospun Fibers Electrospun Fiber Protocol

Silk Thread Silk Thread Protocol Outline Construction of the Synthetic Spider Silk Multimeric Gene Cloning of the spider silk multimer into the expression vector Gene Expression of pETsilk E.coli Cell Lysis Silk Protein Purification Using IMAC Dialysis Protein Analysis Amino Acid Analysis Overall: Timing of Procedure

Reference Materials Fabrication Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web. Production of MASP1/2 Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.