Team:UCLA/Notebook/Protocols

From 2014.igem.org

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<tr><td><br><b>Equipment for the collection of recombinant silk protocols</b></br><a href="https://static.igem.org/mediawiki/2014/a/ab/Equipment_Protocol.pdf">Equipment Protocol</a></td></tr>
<tr><td><br><b>Equipment for the collection of recombinant silk protocols</b></br><a href="https://static.igem.org/mediawiki/2014/a/ab/Equipment_Protocol.pdf">Equipment Protocol</a></td></tr>
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<tr><td><br> <b>List of Reagents</b></br>
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<a href="https://static.igem.org/mediawiki/2014/9/92/Reagent_Protocol.pdf">Reagent Protocol</a></td></tr>
<tr><td><br> <b>PCR for MASP1 and MASP2 </b></br>
<tr><td><br> <b>PCR for MASP1 and MASP2 </b></br>
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<tr><td><br> <b>Silk Films</b></br>
<tr><td><br> <b>Silk Films</b></br>
<a href="https://static.igem.org/mediawiki/2014/d/d2/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%283%29.pdf">Electrospun fibers</a></td></tr>
<a href="https://static.igem.org/mediawiki/2014/d/d2/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%283%29.pdf">Electrospun fibers</a></td></tr>
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<tr><td><br></br></td></tr>
<tr><td><br></br></td></tr>

Revision as of 02:04, 2 July 2014

iGEM UCLA




Protocols

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.


Equipment for the collection of recombinant silk protocols
Equipment Protocol

List of Reagents
Reagent Protocol

PCR for MASP1 and MASP2
PCR Protocol

Silk Fibroin Extraction
Silk Fibroin Extraction Protocol

Silk Films
Silk Films Protocol

Silk Films
Electrospun fibers


Reference

Materials Fabrication
Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web.


Production of MASP1/2
Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.