Team:UCL/experiments
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<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a></div> | <a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a></div> | ||
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- | <p>We were gratefully provided with a number of plasmids | + | <p>We were gratefully provided with a number of plasmids from a group of researchers at the University of Lisbon, Portugal that contained azo-reductase genes from both <i>B. subtilis</i> and <i>P. aeruginosa</i>. As the DNA concentration of the plasmids we were sent was insufficient to perform PCR amplification on we transformed each of these plasmids into our <i>E. coli</i> NEB5lpha competent cells. After growing the cells overnight we then mini-prepped each of them to obtain plasmids at sufficient concentrations for future experimental work.</p> |
<h4>Experiment 3: Diagnostic digest of azo-reductase plasmids</h4> | <h4>Experiment 3: Diagnostic digest of azo-reductase plasmids</h4> |
Revision as of 15:46, 6 September 2014