Revision as of 15:12, 6 September 2014

Goodbye Azo Dye : iGEM 2014 - University College London

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About our project

Experiments

Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit

Adam Denyer October 15, 2013 Protocols competent cells transformation miniprep digest gel

We began our project by identifying a range of BioBrick parts present in the iGEM 2014 distribution kit which we thought would prove useful to our project. These parts primarily consisted of both constituitive and inducible promoter systems with ribosome binding sites which we could then use in conjunction with our azo-reductase BioBricks to assemble a functional azo dye degrading gene. We also decided that we would use the Red Florescent Protein expresing BioBrick as a control for any further transformation experiments. As the level of DNA present within each plate of the distribution kit is insufficient to perform digest and ligation reactions on it was necessary to transform each of these plasmids into our NEB5alpha competent cells. After growing our transformed cells overnight we then mini-prepped each of them to obtain BioBrick plasmids at suitable concentrations for future experiments.