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Team:UCL/experiments
From 2014.igem.org
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<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a> | <a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a> | ||
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p> | <a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p> | ||
| - | <p>We began our project by identifying a range of BioBrick parts present in the iGEM 2014 distribution kit which we thought would prove useful to our project. These parts primarily consisted of both constituitive and inducible promoter systems with ribosome binding sites which we could then use in conjunction with our azo-reductase BioBricks to assemble a functional azo dye degrading gene.</p> | + | <p>We began our project by identifying a range of BioBrick parts present in the iGEM 2014 distribution kit which we thought would prove useful to our project. These parts primarily consisted of both constituitive and inducible promoter systems with ribosome binding sites which we could then use in conjunction with our azo-reductase BioBricks to assemble a functional azo dye degrading gene. We also decided that we would use the Red Florescent Protein expresing BioBrick as a control for any further transformation experiments. As the level of DNA present within each plat of the distribution kit is insufficient to perform digest and ligation reactions on it was necessary to transform each of these plasmids into our NEB5alpha competent cells.</p> |
<p class="more right"><a class="btn btn-primary" href="">Results →</a></p> | <p class="more right"><a class="btn btn-primary" href="">Results →</a></p> | ||
Revision as of 14:56, 6 September 2014
