Team:UCL/experiments

From 2014.igem.org

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                 <ol>
                 <ol>
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                    <li>
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<li>
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<a href="/Team:UCL/experiments#Expt01">Experiment 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</a>
 +
</li>
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<a href="/Team:UCL/experiments#Expt01">Expt 01: Identifying and Confirming Necessary BioBricks from iGEM Parts Registry / Distribution</a>
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<li>
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<a href="/Team:UCL/experiments#Expt02">Experiment 02: Transforming <i>E. coli</i> with Azo-reductase plasmids</a>
</li>
</li>
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                    <li><a href="/Team:UCL/experiments#Expt02">Expt 02: Isolating and Purifying Genes from Lisbon Plasmids</a> (Transform, MP)</li>
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                    <li><a href="/Team:UCL/experiments#Expt03">Expt 03: Confirming Identity of Genes from Lisbon Plasmids</a> (Digest, gel)</li>
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<li>
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                    <li><a href="/Team:UCL/experiments#Expt04">Expt 04: Converting Lisbon Genes into BioBrick Part (Format)</a> (Primer design/order, (site-directed mutatgenesis) PCR)</li>
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<a href="/Team:UCL/experiments#Expt03">Experiment 03: Diagnostic digest of azo-reductase plasmids</a>
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                     <li><a href="/Team:UCL/experiments#Expt05">Expt 05: Confirming Successful Conversion to BioBirck Format</a> (Subclone: digest, ligate, transform, MP, digest, gel)</li>
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</li>
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                    <li><a href="/Team:UCL/experiments#Expt06">Expt 06: Assembling BioBrick Device(s)</a> (Digest, ligate, transform, MP, digest, gel. Repeat ad infinitum...)</li>
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                    <li><a href="/Team:UCL/experiments#Expt07">Expt 07: Characterisation- Azoreductase Assays</a> (See <a href="https://docs.google.com/document/d/1sU1CWSoi1Anydh2AkGb7ZnfdKoWgiAIJ7hat0gvctS0/edit?usp=sharing">Standard Operating Procedure</a> (SOP))</li>
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<li>
 +
<a href="/Team:UCL/experiments#Expt04">Experiment 04: Creation of azo-reductase BioBrick parts from plasmids</a>
 +
</li>
 +
                      
 +
<li>
 +
<a href="/Team:UCL/experiments#Expt05">Experiment 05: Diagnostic digest of azo-reductase BioBrick parts</a>
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</li>
 +
 
 +
<li>
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<a href="/Team:UCL/experiments#Expt06">Experiment  06: Assembling azo-reductase BioBrick Device(s)</a>
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</li>
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<li>
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<a href="/Team:UCL/experiments#Expt07">Expt 07: Characterisation of azo-reductase BioBrick devices</a>
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</li>
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                 </ol></p>
                 </ol></p>
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<h3>Experiment 1: [WHAT AM I]; Date: [ddmmyy]</h3>
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<p><strong>Protocols</strong><br/>  
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<h3>Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit - Date: [ddmmyy]</h3>
 +
<p><strong>Protocols</strong><br/>  
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
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<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
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<p>senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. <em>Aenean ultricies mi vitae est.</em> Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, <code>commodo vitae</code>, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. <a href="#">Donec non enim</a> in turpis pulvinar facilisis. Ut felis.</p>
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<p>senectus et netus et malesuada</p>
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                <p>Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. <em>Aenean ultricies mi vitae est.</em> Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, <code>commodo vitae</code>, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. <a href="#">Donec non enim</a> in turpis pulvinar facilisis. Ut felis.</p>
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                <p>Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. <em>Aenean ultricies mi vitae est.</em> Mauris placerat eleifend leo. Quisque sit amet est et sapien ullamcorper pharetra. Vestibulum erat wisi, condimentum sed, <code>commodo vitae</code>, ornare sit amet, wisi. Aenean fermentum, elit eget tincidunt condimentum, eros ipsum rutrum orci, sagittis tempus lacus enim ac dui. <a href="#">Donec non enim</a> in turpis pulvinar facilisis. Ut felis.</p>
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<h3>Experiment 2: Transforming <i>E. coli</i> with Azo-reductase plasmids - Date: [ddmmyy]</h3>
 +
<p><strong>Protocols</strong><br/>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
 
 +
<h3>Experiment 3: Diagnostic digest of azo-reductase plasmids - Date: [ddmmyy]</h3>
 +
<p><strong>Protocols</strong><br/>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
 
 +
<h3>Experiment 4: Creation of azo-reductase BioBrick parts from plasmids - Date: [ddmmyy]</h3>
 +
<p><strong>Protocols</strong><br/>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
 
 +
<h3>Experiment 5: Diagnostic digest of azo-reductase BioBrick parts - Date: [ddmmyy]</h3>
 +
<p><strong>Protocols</strong><br/>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
 
 +
<h3>Experiment 6: Assembling azo-reductase BioBrick Device(s) - Date: [ddmmyy]</h3>
 +
<p><strong>Protocols</strong><br/>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
 
 +
<h3>Experiment 7: Characterisation of azo-reductase BioBrick devices - Date: [ddmmyy]</h3>
 +
<p><strong>Protocols</strong><br/>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">competent cells</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">transformation</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">miniprep</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">digest</span></a>
 +
<a href="/Team:UCL/protocols"><span class="label label-warning">gel</span></a></p>
 +
<p>senectus et netus et malesuada</p>
 +
 
             </div>
             </div>
             <!-- close span9 primary-column-->
             <!-- close span9 primary-column-->

Revision as of 13:05, 5 September 2014

Goodbye Azo Dye : iGEM 2014 - University College London

 

About our project

Experiments

  1. Experiment 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit
  2. Experiment 02: Transforming E. coli with Azo-reductase plasmids
  3. Experiment 03: Diagnostic digest of azo-reductase plasmids
  4. Experiment 04: Creation of azo-reductase BioBrick parts from plasmids
  5. Experiment 05: Diagnostic digest of azo-reductase BioBrick parts
  6. Experiment 06: Assembling azo-reductase BioBrick Device(s)
  7. Expt 07: Characterisation of azo-reductase BioBrick devices

Experiment 1: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 2: Transforming E. coli with Azo-reductase plasmids - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 3: Diagnostic digest of azo-reductase plasmids - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 4: Creation of azo-reductase BioBrick parts from plasmids - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 5: Diagnostic digest of azo-reductase BioBrick parts - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 6: Assembling azo-reductase BioBrick Device(s) - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Experiment 7: Characterisation of azo-reductase BioBrick devices - Date: [ddmmyy]

Protocols
competent cells transformation miniprep digest gel

senectus et netus et malesuada

Contact Us

University College London - Gower Street - London - WC1E 6BT - Biochemical Engineering Department
phone: +44 (0)20 7679 2000
email: ucligem2014@gmail.com

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