Team:UC-Santa Cruz-BioE/Project

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UC-Santa_Cruz-BioE/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<p><a href="https://2014.igem.org/wiki/index.php?title=Team:UC-Santa_Cruz-BioE/Project&action=edit"style="color:#FFFFFF; position:absolute; left:400px; top:62px; z-index:1; "> Click here  to edit this page!</a></p>
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<span style="color:#191970;font-family:Arial;font-size:16px;"><h1>Designing a Microbial Fuel Cell</h1><!..A HEADING!..></span></div>
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<a href="https://2014.igem.org/Team:UC-Santa_Cruz-BioE"style="color:#000000">Home </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UC-Santa_Cruz-BioE"style="color:#000000"> Official Team Profile </a></td>
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     A microbial fuel cell (MFC) uses bacteria to break down organic compounds found in waste water and generate an electric current. This is a sustainable way to generate power from waste material, with the potential of achieving over 50% energy efficiency. Our project will focus on genetically engineering the bacteria Shewanella oneidensis in ways that will make the microbial fuel cell more efficient. Our hopes that waste water can be inoculated with our transformed MR-1 to fuel the MFC.
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     A microbial fuel cell (MFC) uses bacteria to break down organic compounds found in waste water and generate an electric current. This is a sustainable way to generate power from waste material, with the potential of achieving over 50% energy efficiency. Our project will focus on genetically engineering the bacteria Shewanella oneidensis in ways that will make the microbial fuel cell more efficient.
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    According to the 2013 International Energy Outlook, energy demands will increase 56% by 2040. This rapidly growing demand for energy has sparked a search for sustainable and renewable energy sources. While many technologies are being developed to address this, some of the most intriguing are bioelectrochemical systems like the microbial fuel cell (MFC). The distinctiveness of bioelectrochemical systems come from their ability to simultaneously take on two ecological vices with: sustainable energy production, and waste-water treatment. The basics of how bacteria in an MFC produce electricity are understood, however we feel more research needs to be done to increase efficiency at the microbial level.
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According to the 2013 International Energy Outlook, energy demands will increase 56% by 2040. This rapidly growing demand for energy has sparked a search for sustainable and renewable energy sources. While many technologies are being developed to address this, some of the most intriguing are bioelectrochemical systems like the microbial fuel cell (MFC). The distinctiveness of bioelectrochemical systems come from their ability to simultaneously take on two ecological vices with: sustainable energy production, and waste-water treatment. The basics of how bacteria in an MFC produce electricity are understood, however we feel more research needs to be done to increase efficiency at the microbial level.
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    The most popular research for microbial fuel cells has been poised towards increasing the power density using more state-of-the-art synthetic materials in the structural design. Our project will focus on another aspect: modifying the microbes which are responsible for generating the electricity. Many of the current limiting factors of MFC performance comes from the bacteria themselves.  
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     The most popular research for microbial fuel cells has been poised towards increasing the power density using more state-of-the-art synthetic materials in the structural design. Our project will focus on another aspect: modifying the microbes which are responsible for generating the electricity. Many of the current limiting factors of MFC performance comes from the bacteria themselves. We plan to address these factors with our two project goals.
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     The first and most apparent limiting factor in an MFC is the surface area of the anode. The bacteria can only generate electricity when it is in direct contact with, or in close proximity to the surface of the anode. To take full advantage of the limited surface area, it is best for the bacteria to grow in a dense film called a biofilm. This leads to our goal:
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    The first and most apparent limiting factor in an MFC is the surface area of the anode. The bacteria can only generate electricity when it is in direct contact with, or in close proximity to the surface of the anode. To take full advantage of the limited surface area, it is best for the bacteria to grow in a dense film called a biofilm. This leads to our first goal:
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<li>Increase biofilm growth rate of <i>Shewanella oneidensis</i> on the MFC anode.</li>
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1. Increase biofilm growth rate of Shewanella oneidensis on the MFC anode.
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    We will also focus on the bacteria's ability to transfer electrons from the compounds in the waste water to the anode. There are many pathways which the bacteria can use to facilitate this, however studies have shown that Shewanella does not utilize them in the most efficient way. Much of inefficiency is due to the generation an excretion of Acetate. This is our second goal:
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<p>We will focus on the bacteria's ability to transfer electrons from the compounds in the waste water to the anode. From [7], we learned that biofilm degradation occurs in anaerobic conditions. In [7], the gene VcCD00034807 from Vibrio chlorea was expressed in MR-1 and shown to recover biofilm growth in anaerobic conditions. We plan to transform replicate the experiment done in [7] and grow the bacteria in an MFC to see if we can get a thicker biofilm and higher voltage output. Thickness will be measured by taking stack images with a confocal microscope and acquire the data using a digital multimeter and the ExcelINX software.
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Results not available at this time. Will be presented at Jamboree.
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2. Alter the metabolism used by the bacteria to utilize the energy lost by Acetate generation
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<h3>References </h3>
 
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<li>Korneel Rabaey, ed. Bioelectrochemical systems: from extracellular electron transfer to biotechnological application. IWA publishing, 2010.</li>
 
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<li>Franks, Ashley E., and Kelly P. Nevin. "Microbial fuel cells, a current review." Energies 3.5 (2010): 899-919.</li>
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<li>1. Korneel Rabaey, ed. Bioelectrochemical systems: from extracellular electron transfer to biotechnological application. IWA publishing, 2010.</li>
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<li>Brutinel ED, Gralnick JA. Anomalies of the anaerobic tricarboxylic acid cycle in Shewanella oneidensis revealed by Tn-seq. Mol Microbiol. 2012 Oct;86(2):273-83. doi: 10.1111/j.1365-2958.2012.08196.x. Epub 2012 Aug 27. PubMed PMID: 22925268. </li>
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<li>2. Franks, Ashley E., and Kelly P. Nevin. "Microbial fuel cells, a current review." Energies 3.5 (2010): 899-919.</li>
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<li>Papagianni M. Recent advances in engineering the central carbon metabolism of industrially important bacteria. Microb Cell Fact. 2012 Apr 30;11:50. doi: 10.1186/1475-2859-11-50. Review. PubMed PMID: 22545791; PubMed Central PMCID: PMC3461431</li>
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<li>3. Brutinel ED, Gralnick JA. Anomalies of the anaerobic tricarboxylic acid cycle in <i>Shewanella oneidensis</i> revealed by Tn-seq. Mol Microbiol. 2012 Oct;86(2):273-83. doi: 10.1111/j.1365-2958.2012.08196.x. Epub 2012 Aug 27. PubMed PMID: 22925268. </li>
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<li>Rabaey K, Verstraete W. Microbial fuel cells: novel biotechnology for energy generation. Trends Biotechnol. 2005 Jun;23(6):291-8. Review. PubMed PMID: 15922081. </li>
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<li>4. Papagianni M. Recent advances in engineering the central carbon metabolism of industrially important bacteria. Microb Cell Fact. 2012 Apr 30;11:50. doi: 10.1186/1475-2859-11-50. Review. PubMed PMID: 22545791; PubMed Central PMCID: PMC3461431</li>
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<li>Beliaev, Alex S., et al. "Gene and protein expression profiles of Shewanella oneidensis during anaerobic growth with different electron acceptors." Omics: a journal of integrative biology 6.1 (2002): 39-60.</li>
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<li>5. Rabaey K, Verstraete W. Microbial fuel cells: novel biotechnology for energy generation. Trends Biotechnol. 2005 Jun;23(6):291-8. Review. PubMed PMID: 15922081. </li>
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<li>Thormann, Kai M., et al. "Control of formation and cellular detachment from Shewanella oneidensis MR-1 biofilms by cyclic di-GMP." Journal of Bacteriology 188.7 (2006): 2681-2691.</li>
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<li>6. Beliaev, Alex S., et al. "Gene and protein expression profiles of <i>Shewanella oneidensis</i> during anaerobic growth with different electron acceptors." Omics: a journal of integrative biology 6.1 (2002): 39-60.</li>
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<li>7. Thormann, Kai M., et al. "Control of formation and cellular detachment from <i>Shewanella oneidensis</i> MR-1 biofilms by cyclic di-GMP." Journal of Bacteriology 188.7 (2006): 2681-2691.</li>
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<p>(Referred to in <a href="https://igem.org/Team.cgi?year=2014&team_name=UC-Santa_Cruz-BioE">Abstract</a>)</p>
<p>(Referred to in <a href="https://igem.org/Team.cgi?year=2014&team_name=UC-Santa_Cruz-BioE">Abstract</a>)</p>
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<li>8. Ching, Leang, and Nikhil S. Malvankar. "Engineering Geobacter Sulffureducens to Produce a Highly Cohesive Conductive Matrix with Enhanced Capacity for Current Production." Energy and Environmental Science (2013): 1901-908. Web.</li>
<li>8. Ching, Leang, and Nikhil S. Malvankar. "Engineering Geobacter Sulffureducens to Produce a Highly Cohesive Conductive Matrix with Enhanced Capacity for Current Production." Energy and Environmental Science (2013): 1901-908. Web.</li>
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<p> You can use these subtopics to further explain your project</p>
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<div id="pictureofMFC" style=" position: absolute; left:1100px; top:474px; width: 400px; text-align=left; " >
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<figcaption style="color:#FFFFFF"> Our Microbial Fuel Cell</figcaption>
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It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.  
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Latest revision as of 03:53, 18 October 2014


Project

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ProjectOur Project

Designing a Microbial Fuel Cell

A microbial fuel cell (MFC) uses bacteria to break down organic compounds found in waste water and generate an electric current. This is a sustainable way to generate power from waste material, with the potential of achieving over 50% energy efficiency. Our project will focus on genetically engineering the bacteria Shewanella oneidensis in ways that will make the microbial fuel cell more efficient. Our hopes that waste water can be inoculated with our transformed MR-1 to fuel the MFC.


According to the 2013 International Energy Outlook, energy demands will increase 56% by 2040. This rapidly growing demand for energy has sparked a search for sustainable and renewable energy sources. While many technologies are being developed to address this, some of the most intriguing are bioelectrochemical systems like the microbial fuel cell (MFC). The distinctiveness of bioelectrochemical systems come from their ability to simultaneously take on two ecological vices with: sustainable energy production, and waste-water treatment. The basics of how bacteria in an MFC produce electricity are understood, however we feel more research needs to be done to increase efficiency at the microbial level.


The most popular research for microbial fuel cells has been poised towards increasing the power density using more state-of-the-art synthetic materials in the structural design. Our project will focus on another aspect: modifying the microbes which are responsible for generating the electricity. Many of the current limiting factors of MFC performance comes from the bacteria themselves.


The first and most apparent limiting factor in an MFC is the surface area of the anode. The bacteria can only generate electricity when it is in direct contact with, or in close proximity to the surface of the anode. To take full advantage of the limited surface area, it is best for the bacteria to grow in a dense film called a biofilm. This leads to our goal:


  • Increase biofilm growth rate of Shewanella oneidensis on the MFC anode.

We will focus on the bacteria's ability to transfer electrons from the compounds in the waste water to the anode. From [7], we learned that biofilm degradation occurs in anaerobic conditions. In [7], the gene VcCD00034807 from Vibrio chlorea was expressed in MR-1 and shown to recover biofilm growth in anaerobic conditions. We plan to transform replicate the experiment done in [7] and grow the bacteria in an MFC to see if we can get a thicker biofilm and higher voltage output. Thickness will be measured by taking stack images with a confocal microscope and acquire the data using a digital multimeter and the ExcelINX software.
Results not available at this time. Will be presented at Jamboree.


References

  • 1. Korneel Rabaey, ed. Bioelectrochemical systems: from extracellular electron transfer to biotechnological application. IWA publishing, 2010.
  • 2. Franks, Ashley E., and Kelly P. Nevin. "Microbial fuel cells, a current review." Energies 3.5 (2010): 899-919.
  • 3. Brutinel ED, Gralnick JA. Anomalies of the anaerobic tricarboxylic acid cycle in Shewanella oneidensis revealed by Tn-seq. Mol Microbiol. 2012 Oct;86(2):273-83. doi: 10.1111/j.1365-2958.2012.08196.x. Epub 2012 Aug 27. PubMed PMID: 22925268.
  • 4. Papagianni M. Recent advances in engineering the central carbon metabolism of industrially important bacteria. Microb Cell Fact. 2012 Apr 30;11:50. doi: 10.1186/1475-2859-11-50. Review. PubMed PMID: 22545791; PubMed Central PMCID: PMC3461431
  • 5. Rabaey K, Verstraete W. Microbial fuel cells: novel biotechnology for energy generation. Trends Biotechnol. 2005 Jun;23(6):291-8. Review. PubMed PMID: 15922081.
  • 6. Beliaev, Alex S., et al. "Gene and protein expression profiles of Shewanella oneidensis during anaerobic growth with different electron acceptors." Omics: a journal of integrative biology 6.1 (2002): 39-60.
  • 7. Thormann, Kai M., et al. "Control of formation and cellular detachment from Shewanella oneidensis MR-1 biofilms by cyclic di-GMP." Journal of Bacteriology 188.7 (2006): 2681-2691.

Additional Resources

(Referred to in Abstract)

  • 8. Ching, Leang, and Nikhil S. Malvankar. "Engineering Geobacter Sulffureducens to Produce a Highly Cohesive Conductive Matrix with Enhanced Capacity for Current Production." Energy and Environmental Science (2013): 1901-908. Web.
MFC
Our Microbial Fuel Cell
plate
Plated Shewanella did not exhibit ampicillin resistance.
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Plasmid Extraction

Retrieved from "http://2014.igem.org/Team:UC-Santa_Cruz-BioE/Project"