Team:UANL Mty-Mexico/project/abstract

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Project

Abstract

The Reprogrammator Project

In order to be capable of controlling transformed bacteria and reprogram its function as many times as would be required, we designed a system consisting of a bacteriophage that infects just one specific strain of bacteria and has on its genome the information to codify a TALEN/ZFN enzyme and a reporter gene. With this tools (TALEN/ZFN) we can target a specific sequence of the host's plasmids to degrade them. The bacteriophages will replace the plasmids, giving it a new function or characteristic that could be a totally new one.

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-<p><b>Thermonator III: The crop guardian</b></p>
+<p><b>The Reprogrammator Project</b></p>
-<p align="justify">Our project is about a genetic engineered machine in <i>E. coli</i> with the ability to produce Vip3ca3 which acts as a pesticide protein. Vip3ca3 production will be regulated by specific temperatures in order to avoid overproduction and it will show activity against target organisms Coleoptera and Lepidoptera, which are related to a local problem concerning potato crops. The Vip3ca3 production is regulated with a constitutive promoter and a riboswitch that initiate translation around 32°C. Since we want to produce the Vip3ca3 below the 32°C, we use a set of promoter-repressors in order to invert the activation of the protein production. This model may be used as a regulator in future transgenic plant generations for the production of substances against plagues, avoiding pesticide overproduction, thus reducing the effect in Non-Target Organisms and bioaccumulation and can be used with different temperature ranges and/or different proteins to attack other target organisms.</p>
+<p align="justify">In order to be capable of controlling transformed bacteria and reprogram its function as many times as would be required, we designed a system consisting of a bacteriophage that infects just one specific strain of bacteria and has on its genome the information to codify a TALEN/ZFN enzyme and a reporter gene. With this tools (TALEN/ZFN) we can target a specific sequence of the host's plasmids to degrade them. The bacteriophages will replace the plasmids, giving it a new function or characteristic that could be a totally new one.</p>
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