"
Team:Tuebingen/Notebook/Protocols/LB medium
From 2014.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 41: | Line 41: | ||
<ol> | <ol> | ||
| - | <li> Add the reagents to 800ml | + | <li> Add the reagents to 800ml H<sub>2</sub>O</li> |
| - | <li> Adjust pH to 7.5 with NaOH | + | <li> Adjust pH to 7.5 with NaOH</li> |
| - | </li> | + | <li> Adjust volume to 1L with dH<sub>2</sub>O</li> |
| - | <li> Adjust volume to 1L with | + | |
<li> Sterilize by autoclaving</li> | <li> Sterilize by autoclaving</li> | ||
Latest revision as of 15:32, 17 October 2014
Protocols
LB medium
Reagents
| 10 g | Bacto-tryptone |
| 5 g | yeast extract |
| 10 g | NaCl |
Procedure
- Add the reagents to 800ml H2O
- Adjust pH to 7.5 with NaOH
- Adjust volume to 1L with dH2O
- Sterilize by autoclaving
