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Team:Tuebingen/Notebook/Protocols/LB medium
From 2014.igem.org
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| - | + | <h1>Protocols</h1> | |
<h3>LB medium</h3> | <h3>LB medium</h3> | ||
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</table> | </table> | ||
| + | <p> </p> | ||
<h4>Procedure</h4> | <h4>Procedure</h4> | ||
<ol> | <ol> | ||
| - | <li> Add the reagents to 800ml | + | <li> Add the reagents to 800ml H<sub>2</sub>O</li> |
| - | + | ||
| - | + | ||
| - | <li> Adjust volume to 1L with | + | <li> Adjust pH to 7.5 with NaOH</li> |
| + | <li> Adjust volume to 1L with dH<sub>2</sub>O</li> | ||
| - | <li> Sterilize by autoclaving | + | <li> Sterilize by autoclaving</li> |
</ol> | </ol> | ||
</div> | </div> | ||
Latest revision as of 15:32, 17 October 2014
Protocols
LB medium
Reagents
| 10 g | Bacto-tryptone |
| 5 g | yeast extract |
| 10 g | NaCl |
Procedure
- Add the reagents to 800ml H2O
- Adjust pH to 7.5 with NaOH
- Adjust volume to 1L with dH2O
- Sterilize by autoclaving
