Team:Tuebingen/Notebook/Protocols/LB medium

From 2014.igem.org

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<div id="TueContent">
<div id="TueContent">
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<h1>Protocols</h1>
<h3>LB medium</h3>
<h3>LB medium</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">10 g</td>
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    <td>Bacto-tryptone</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">5 g</td>
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    <td>yeast extract</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">10 g</td>
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    <td>NaCl</td>
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  </tr>
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</table>
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<p>&nbsp;</p>
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<h4>Procedure</h4>
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<ol>
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  <li> Add the reagents to 800ml H<sub>2</sub>O</li>
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  <li> Adjust pH to 7.5 with NaOH</li>
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  <li> Adjust volume to 1L with dH<sub>2</sub>O</li>
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  <li> Sterilize by autoclaving</li>
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</ol>
</div>
</div>

Latest revision as of 15:32, 17 October 2014


Protocols

LB medium

Reagents

10 g Bacto-tryptone
5 g yeast extract
10 g NaCl

 

Procedure

  1. Add the reagents to 800ml H2O
  2. Adjust pH to 7.5 with NaOH
  3. Adjust volume to 1L with dH2O
  4. Sterilize by autoclaving

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