Team:Tuebingen/Notebook/Protocols/LB medium

From 2014.igem.org

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Revision as of 15:25, 17 October 2014


Protocols

LB medium

Reagents

10 g Bacto-tryptone
5 g yeast extract
10 g NaCl

 

Procedure

  1. Add the reagents to 800ml H2O
  2. Adjust pH to 7.5 with NaOH
  3. Adjust volume to 1L with dH2O
  4. Sterilize by autoclaving

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