Team:Toulouse/Safety

From 2014.igem.org

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<td style="border:1px solid black"><b><p class ="texte">Risk group</b></td>
<td style="border:1px solid black"><b><p class ="texte">Risk group</b></td>
<td style="border:1px solid black"><b><p class ="texte">Risk Group Source</b></td>
<td style="border:1px solid black"><b><p class ="texte">Risk Group Source</b></td>
-
<td style="border:1px solid black"><b><p class ="texte">Disease risk to humans ?</b></td>
+
<td style="border:1px solid black"><b><p class ="texte">Disease risk for humans ?</b></td>
</tr>
</tr>
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<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-7140.html">DSMZ</p></a>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-7140.html">DSMZ</p></a>
-
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
+
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
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<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-23778.html">DSMZ</p></a>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-23778.html">DSMZ</p></a>
-
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
+
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
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<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-63263.html">DSMZ</p></a>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-63263.html">DSMZ</p></a>
-
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
+
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
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<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-1962.html">DSMZ</p></a>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-1962.html">DSMZ</p></a>
-
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
+
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
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<!--6eme ligne-->
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<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-768.html">DSMZ</p></a>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-768.html">DSMZ</p></a>
-
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
+
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
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<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte">1</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-820.html">DSMZ</p></a>
<td style="border:1px solid black"><p class ="texte"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-820.html">DSMZ</p></a>
-
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
+
<td style="border:1px solid black"><p class ="texte">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
</table>
</table>
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<td style="border:1px solid black"><p class ="texte"><a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</p></td>
<td style="border:1px solid black"><p class ="texte">N-acetylatedGlucosamine based chemotaxis for <i>Bacillus subtilis</i></p></td>
<td style="border:1px solid black"><p class ="texte">N-acetylatedGlucosamine based chemotaxis for <i>Bacillus subtilis</i></p></td>
-
<td style="border:1px solid black"><p class ="texte">We order this gene from a synthesis company (Eurofins)</p></td>
+
<td style="border:1px solid black"><p class ="texte">We ordered this gene from a synthesis company (Eurofins)</p></td>
<td style="border:1px solid black"><p class ="texte">This part allows the bacterium to reach the fungus</p></td>
<td style="border:1px solid black"><p class ="texte">This part allows the bacterium to reach the fungus</p></td>
</tr>
</tr>
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<td style="border:1px solid black"><p class ="texte"><a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</p></td>
<td style="border:1px solid black"><p class ="texte">RBS - Antifungal GAFP-1</p></td>
<td style="border:1px solid black"><p class ="texte">RBS - Antifungal GAFP-1</p></td>
-
<td style="border:1px solid black"><p class ="texte">We order this gene from a synthesis company (Eurofins)</p></td>
+
<td style="border:1px solid black"><p class ="texte">We ordered this gene from a synthesis company (Eurofins)</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacteria is able to kill fungus.</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacteria is able to kill the fungus.</p></td>
</tr>
</tr>
<!--5eme ligne-->
<!--5eme ligne-->
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<td style="border:1px solid black"><p class ="texte"><a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</p></td>
<td style="border:1px solid black"><p class ="texte"><a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</p></td>
<td style="border:1px solid black"><p class ="texte">RBS - Antifungal D4E1 - Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">RBS - Antifungal D4E1 - Double terminator</p></td>
-
<td style="border:1px solid black"><p class ="texte">We order this gene from a synthesis company (Eurofins)</p></td>
+
<td style="border:1px solid black"><p class ="texte">We ordered this gene from a synthesis company (Eurofins)</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacteria is able to kill fungus.</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacteria is able to kill the fungus.</p></td>
</tr>
</tr>
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<!--6eme ligne-->
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<td style="border:1px solid black"><p class ="texte">Double expression cassette</p></td>
<td style="border:1px solid black"><p class ="texte">Double expression cassette</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part allows the bacterium to reach and bind the fungus</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part allows the bacterium to reach and bind to the fungus</p></td>
</tr>
</tr>
<!--9eme ligne-->
<!--9eme ligne-->
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<td style="border:1px solid black"><p class ="texte">RBS + Antifungal GAFP-1 + Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">RBS + Antifungal GAFP-1 + Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill fungus</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill the fungus</p></td>
</tr>
</tr>
<!--10eme ligne-->
<!--10eme ligne-->
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<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill fungus</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill the fungus</p></td>
</tr>
</tr>
<!--11eme ligne-->
<!--11eme ligne-->
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<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p></td>
<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill fungus</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill the fungus</p></td>
</tr>
</tr>
<!--12eme ligne-->
<!--12eme ligne-->
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<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub>-SpoVG + EcAMP-1</p></td>
<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub>-SpoVG + EcAMP-1</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill fungus</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill the fungus</p></td>
</tr>
</tr>
<!--13eme ligne-->
<!--13eme ligne-->
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<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub>-spoVG + EcAMP-1 + Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub>-spoVG + EcAMP-1 + Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill fungus</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill the fungus</p></td>
</tr>
</tr>
<!--15eme ligne-->
<!--15eme ligne-->
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<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> - RBS - Antifungal GAFP-1 - RBS - Antifungal D4E1 - Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> - RBS - Antifungal GAFP-1 - RBS - Antifungal D4E1 - Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill fungus.</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill the fungus.</p></td>
</tr>
</tr>
<!--15eme ligne-->
<!--15eme ligne-->
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<td style="border:1px solid black"><p class ="texte">Promotor P<sub>lepA</sub> + RBS spoVG</p></td>
<td style="border:1px solid black"><p class ="texte">Promotor P<sub>lepA</sub> + RBS spoVG</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part provide us a high level of translation.</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part provides us a high level of translation.</p></td>
</tr>
</tr>
<!--15eme ligne-->
<!--15eme ligne-->
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<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> + RBS + Antifungal EcAMP-1 + Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">P<sub>veg</sub> + RBS + Antifungal EcAMP-1 + Double terminator</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill fungus.</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part produces a fungicide. By this way, the bacterium is able to kill the fungus.</p></td>
</tr>
</tr>
<!--15eme ligne-->
<!--15eme ligne-->
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<td style="border:1px solid black"><p class ="texte">Integrative plasmid for <i>Bacillus subtilis</i></p></td>
<td style="border:1px solid black"><p class ="texte">Integrative plasmid for <i>Bacillus subtilis</i></p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
<td style="border:1px solid black"><p class ="texte">We made it</p></td>
-
<td style="border:1px solid black"><p class ="texte">This part is an integration vector.</p></td>
+
<td style="border:1px solid black"><p class ="texte">This part is an integrative vector.</p></td>
</tr>
</tr>
</table>
</table>
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<p class="title1" id="select4">Team safety</p>
<p class="title1" id="select4">Team safety</p>
<p class="title2">Safety in INSA de Toulouse</p>
<p class="title2">Safety in INSA de Toulouse</p>
-
<p class="texte">INSA de Toulouse is a public school for engineers. The biosafety guidelines are not specific to our institution, the French regulations are applied.<br>
+
<p class="texte">INSA de Toulouse is a public school for engineers. The biosafety guidelines are not specific to our institution; the French regulations are applied.<br>
-
The regulation about the workers' prevention against risks resulting from their exposure to pathogenic biological agents (Decree No. 94-352 of 4 May 1994) includes microorganisms, cell cultures and human endoparasites which may cause infection, allergy or toxicity. <br>
+
The regulation about the workers' prevention against risks resulting from their exposure to pathogenic biological agents (Decree No. 94-352 of 4 May 1994) includes microorganisms, cell cultures and human endoparasites which may cause infections, allergies or toxicity. <br>
This Decree is the French transposition of the Directive 90/679 / EEC and is also transcribed in the Labour Code (Articles L4421-1 R4421-1 to R4427-5.)<br>
This Decree is the French transposition of the Directive 90/679 / EEC and is also transcribed in the Labour Code (Articles L4421-1 R4421-1 to R4427-5.)<br>
-
The <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000000465273&dateTexte=&categorieLien=id">Decree of the 16 July 2007</a> describes the technical preventive measures (including containment) to set up in research laboratories, education, analysis, anatomy and surgical pathology, autopsy rooms, and industrial and agricultural facilities where workers are likely to be exposed to biological pathogens.<br>
+
The <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000000465273&dateTexte=&categorieLien=id">Decree of the 16th July 2007</a> describes the technical preventive measuresto set up in research laboratories (including containment), education, analysis, anatomy and surgical pathology, autopsy rooms, and industrial and agricultural facilities where workers are likely to be exposed to biological pathogens.<br>
The rules of health, safety, and preventive medicine applied in public services in France (and thus in all public facilities working in scientific and technological domains) are set out in the <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=LEGITEXT000006063791">Decree No. 82-453</a>. This decree refers to the Labour Code, Public Health Code and Environmental Code.<br>
The rules of health, safety, and preventive medicine applied in public services in France (and thus in all public facilities working in scientific and technological domains) are set out in the <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=LEGITEXT000006063791">Decree No. 82-453</a>. This decree refers to the Labour Code, Public Health Code and Environmental Code.<br>
The <a href="http://legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000024584737&categorieLien=id">Decree No. 2011-1177</a> is related to the use of genetically modified organisms.<br>
The <a href="http://legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000024584737&categorieLien=id">Decree No. 2011-1177</a> is related to the use of genetically modified organisms.<br>
<br>
<br>
-
There is no one in charge of the biological safety at the INSA de Toulouse. However there is an organization which includes one prevention advisor and several prevention assistants in each of the structure. As far as the LISBP (our structure) is concerned, Nathalie Doubrovine is the prevention assistant.<br>
+
There is no one in charge of the biological safety at the INSA de Toulouse. However there is an organization which includes one prevention advisor and several prevention assistants in every structure. As far as the LISBP (our structure) is concerned, Nathalie Doubrovine is the prevention assistant.<br>
-
We have already discussed our project with her. She has given us the safety training and she will give us advices about how to respond to an imminent danger.<br>
+
We have already discussed our project with her. She has given us the safety training and advices about how to respond to an imminent danger.<br>
She raised the problem of GMO and the related policy. Indeed she told us there is a special procedure for the destruction of GMO: we cannot wash this kind of organism down the drain.<br>
She raised the problem of GMO and the related policy. Indeed she told us there is a special procedure for the destruction of GMO: we cannot wash this kind of organism down the drain.<br>
-
We were also informed about the potential chemical and microbiological risks with her.<br>
+
We were also informed about the potential chemical and microbiological risks thanks to her.<br>
<br>
<br>
The laboratory safety training requirements of the LISBP are detailed into the Rules of Procedure of the LISBP.<br>
The laboratory safety training requirements of the LISBP are detailed into the Rules of Procedure of the LISBP.<br>
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The training is divided into two parts. The first one is a training concerning general risk prevention into research laboratories. This one is made individually thanks to the software NEO and with the explanations of the prevention assistant. The second one is a training about the techniques used during the occupation.<br>
The training is divided into two parts. The first one is a training concerning general risk prevention into research laboratories. This one is made individually thanks to the software NEO and with the explanations of the prevention assistant. The second one is a training about the techniques used during the occupation.<br>
<br>
<br>
-
All the team members have received this safety training. It lasts 1h30. It concerns fire risks, biological and chemical risks and good laboratory practices. We have made this training individually with software designed by the CNRS and INSERM named NEO. This training ends with a questionnaire to check our knowledge. This questionnaire reminds us the basic safety rules in a molecular biology lab and gives us new knowledge about safety.<br>
+
All the team members have received this safety training. It lasts 1h30. It concerns fire risks, biological and chemical risks and good laboratory practices. We have made this training individually with a software designed by the CNRS and INSERM named NEO. This training ends with a questionnaire to check our knowledge. This questionnaire reminds us the basic safety rules in a molecular biology lab and gives us new knowledge about safety.<br>
We have also made a training to know how to sort out biological waste and how they are treated. We also learned that we cannot use autoclave by ourselves because a specific training is needed. We did not do this specific training.<br>
We have also made a training to know how to sort out biological waste and how they are treated. We also learned that we cannot use autoclave by ourselves because a specific training is needed. We did not do this specific training.<br>
</p>
</p>
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There is also others precaution when working with biological organisms:<br>
There is also others precaution when working with biological organisms:<br>
- We need to wash our hands regularly.<br>
- We need to wash our hands regularly.<br>
-
- It is compulsory to wear gloves except when working with an electric burner.<br>
+
- It is compulsory to wear gloves except with the use of an electric burner.<br>
-
- In some case (UV light, projection risk), it is compulsory to wear protection glasses.<br>
+
- In some cases (UV light, projection risk), it is compulsory to wear protection glasses.<br>
<br>
<br>
Indeed, different machine are used to keep a sterile area.</p>
Indeed, different machine are used to keep a sterile area.</p>
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<p class="title4">Ethidium Bromide</p>
<p class="title4">Ethidium Bromide</p>
-
<p class="texte">We have a dark room dedicated to the use of EtBr and UV. This room is key-closed and everyone entering into the room must wear gloves, glasses and lab coat. Everything which is in contact directly with something in this room has to stay here.<br>
+
<p class="texte">We have a dark room dedicated to the use of EtBr and UV. This room is key-closed and everyone entering into the room must wear gloves, glasses and lab coat. Everything which is in direct contact with something in this room has to stay here.<br>
<img width="150px" src="http://2014.igem.org/wiki/images/7/75/EtBr_room.JPG"><br><br>
<img width="150px" src="http://2014.igem.org/wiki/images/7/75/EtBr_room.JPG"><br><br>
<img width="150px" src="http://2014.igem.org/wiki/images/4/45/EtBr_door.JPG"><br><br>
<img width="150px" src="http://2014.igem.org/wiki/images/4/45/EtBr_door.JPG"><br><br>
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<p class="title4">Biological safety cabinet</p>
<p class="title4">Biological safety cabinet</p>
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<p class="texte">We use a Biological safety cabinet (FASTER – Ultrasafe) to manipulate into a sterile area and thus avoid external contamination by unwanted microorganisms. We clean the bench of the BSC with ethanol before each manipulation. We also clean the BSC completely every two weeks. Because we work with fungi, we have to be very careful with the cleanliness of the BSC.</p>
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<p class="texte">We use a Biological safety cabinet (FASTER – Ultrasafe) to manipulate into a sterile area and thus avoid external contamination by unwanted microorganisms. We clean the bench of the BSC with ethanol before and after each manipulation. We also clean the BSC completely every two weeks. Because we work with fungi, we have to be very careful with the cleanliness of the BSC.</p>
<p class="title4">Electric burner</p>
<p class="title4">Electric burner</p>
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<p class="title3">Risks to the safety and health of team members, or other people working in the lab</p>
<p class="title3">Risks to the safety and health of team members, or other people working in the lab</p>
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<p class="texte">We work with the B. subtilis and E. coli chassis. These organisms are non-pathogenic. Moreover the used parts are also non-pathogenic (the bio-fungicides produced are harmless for humans).<br>
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<p class="texte">We work with the <I>B. subtilis </I> and <i>E. coli</i> chassis. These organisms are non-pathogenic. Moreover the used parts are also non-pathogenic (the bio-fungicides produced are harmless for humans).<br>
On one hand, there is a major biological risk because of the manipulation of DNA and RNA of bacterial organisms.<br>
On one hand, there is a major biological risk because of the manipulation of DNA and RNA of bacterial organisms.<br>
On the other hand the main risk for humans is chemical: we use Ethidium Bromide, hydrochloric acid, soda, solutions from a Miniprep kit, ethanol and bleach.<br>
On the other hand the main risk for humans is chemical: we use Ethidium Bromide, hydrochloric acid, soda, solutions from a Miniprep kit, ethanol and bleach.<br>
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<p class="title3">Risks to the safety and health of the general public</p>
<p class="title3">Risks to the safety and health of the general public</p>
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<p class="texte">If biological materials escape from our lab, the risks regarding safety and health of the general public is low because the parts manipulated are harmless. The only risk is the contact with DNA or RNA from a bacterial organism.<br>
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<p class="texte">If biological materials escape from our lab, the risks regarding safety and health of the general public is low because the manipulated parts are harmless. The only risk is the contact with DNA or RNA from a bacterial organism.<br>
Moreover, we have to be cautious with the use of antibiotics and we must not wash them down the drain.</p>
Moreover, we have to be cautious with the use of antibiotics and we must not wash them down the drain.</p>

Revision as of 13:42, 16 October 2014