Team:Toulouse/Safety

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   <div class="fils-ariane" style="width:100%; height:60px; background:#ededed; margin-top:30px;">
   <div class="fils-ariane" style="width:100%; height:60px; background:#ededed; margin-top:30px;">
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   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Notebook&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Project monitoring</p>  
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   <div style="margin:0 auto; width:960px;">
 +
  <p style="color:#696969; padding-top:20px; font-size:16px; float:left;"> Human practice&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Safety</p>
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  </div>
   </div>
   </div>
    
    
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       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
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<!--TITRE-->
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<div id="column-left">
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<h1 align="center" style="font-size:50px">
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<h3 class="title2" style="margin-top:10px; color:#333;">Summary :</h3>
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Safety
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<ul class="menuleft">
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</h1>
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  <li style="margin-top:25px;"><a href="#select1">iGEM Safety</a></li>
 +
  <li><a href="#select2">Chassis Organism</a></li>
 +
  <li><a href="#select3">New and/or modified coding region</a></li>
 +
  <li><a href="#select4">Team safety</a></li>
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</ul>
 +
</div>
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<br>
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<div class="column-right" style="width:75%; float:right;">
<!--CITATION-->
<!--CITATION-->
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<p align="center"><i>
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<p class="citation">
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"Safety is not just a slogan, it is a way of life"
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"Safety is not just a slogan, it's a way of life"
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</i></p>
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-
 
+
-
<br>
+
-
 
+
-
<!--SUMMARY-->
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-
<p><b>
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-
Summary :</b><br><br>
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-
iGEM Safety<br>
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-
Chassis Organism<br>
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-
New and/or modified coding region<br>
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-
Safety in our lab<br>
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</p>
</p>
<br>
<br>
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<!--PARTIE iGEM SAFETY-->
<!--PARTIE iGEM SAFETY-->
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<p>
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<p class="title1" id="select1">iGEM Safety</p>
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<b>iGEM Safety</b><br>
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<p class ="texte">We filled the iGEM safety form with the help of Nathalie Doubrovine, safety officer at the LISBP.
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Our safety form was approved on September X, 2014 by the iGEM team. We filled in with the help of Nathalie Doubrovine, safety officer at the LISBP.
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</p>
</p>
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<br>
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<!--TABLE CHASSIS ORGANISM-->
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<br>
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<p class="title1" id="select2">Chassis organisms</p>
 +
 
 +
<table style="border-width:1px; border-color:#ccc;width:100%;background-color:#ccc;"
 +
>
 +
<tbody style="background-color:white;">
-
<!--TABLE CHASSIS ORGANISM-->
 
-
<table border="1" width="100%" height="20%" id="Chassis">
 
<!--1ere ligne-->
<!--1ere ligne-->
<tr>
<tr>
-
<td><b>Species name (including strain)</b></td>
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<td style="background-color:#22780F"><p class ="texte" style="color:white; text-align:center; margin: 0 30px;">Species name (including strain)</td>
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<td><b>Risk group</b></td>
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<td style=" background-color:#22780F"><p class ="texte" style="color:white; text-align:center; margin: 0 10px;">Risk group</td>
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<td><b>Risk Group Source</b></td>
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<td style=" background-color:#22780F"><p class ="texte" style="color:white; text-align:center; margin: 0 10px;">Risk Group Source</td>
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<td><b>Disease risk to humans ?</b></td>
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<td style=" background-color:#22780F"><p class ="texte" style="color:white; text-align:center; padding-top: 42px; font-size: 17px; ">Disease risk for humans ?</td>
</tr>
</tr>
<!--2eme ligne-->
<!--2eme ligne-->
<tr>
<tr>
-
<td><i>Escherichia coli MC1061</i></td>
+
<td><i><p class ="texte" style="padding-top: 10px; text-align:center;">Escherichia coli MC1061</i></p></td>
-
<td>1</td>
+
<td><p class ="texte" style="text-align:center">1</p></td>
-
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-7140.html">DSMZ</a>
+
<td><p class ="texte" style="text-align:center"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-7140.html">DSMZ</p></a>
-
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
<!--3eme ligne-->
<!--3eme ligne-->
-
<tr>
+
<tr style=" background-color:#f8f8f8">
-
<td><i>Bacillus subtilis 168</i></td>
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<td><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Bacillus subtilis 168</i></p></td>
-
<td>1</td>
+
<td><p class ="texte" style="text-align:center">1</p></td>
-
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-23778.html">DSMZ</a>
+
<td><p class ="texte" style="text-align:center"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-23778.html">DSMZ</p></a>
-
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
<!--4eme ligne-->
<!--4eme ligne-->
<tr>
<tr>
-
<td><i>Aspergillus brasiliensis 246.65 CBS</i></td>
+
<td><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Aspergillus brasiliensis 246.65 CBS</i></p></td>
-
<td>1</td>
+
<td><p class ="texte" style="text-align:center">1</p></td>
-
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-63263.html">DSMZ</a>
+
<td><p class ="texte" style="text-align:center"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-63263.html">DSMZ</p></a>
-
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
<!--5eme ligne-->
<!--5eme ligne-->
 +
<tr style=" background-color:#f8f8f8">
 +
<td><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Chaetomium globosum 148.51 CBS</i></p></td>
 +
<td><p class ="texte" style="text-align:center">1</p></td>
 +
<td><p class ="texte" style="text-align:center"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-1962.html">DSMZ</p></a>
 +
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
 +
</tr>
 +
<!--6eme ligne-->
<tr>
<tr>
-
<td><i>Chaetomium globosum 148.51 CBS</i></td>
+
<td><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Trichoderma reesei CBS 383.78</i></p></td>
-
<td>1</td>
+
<td><p class ="texte" style="text-align:center">1</p></td>
-
<td><a href="http://www.dsmz.de/catalogues/details/culture/DSM-1962.html">DSMZ</a>
+
<td><p class ="texte" style="text-align:center"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-768.html">DSMZ</p></a>
-
<td>No. These organisms do not cause disease in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
 +
</tr>
 +
<!--7eme ligne-->
 +
<tr style=" background-color:#f8f8f8">
 +
<td><p class ="texte" style="padding-top: 10px; text-align:center;"><i>Aspergillus nidulans CBS 124.59</i></p></td>
 +
<td><p class ="texte" style="text-align:center">1</p></td>
 +
<td><p class ="texte" style="text-align:center"><a href="http://www.dsmz.de/catalogues/details/culture/DSM-820.html">DSMZ</p></a>
 +
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">No. These organisms do not cause diseases in healthy adult humans. (However, they might cause diseases in young children, elderly people, or people with immune system deficiencies.)</p></td>
</tr>
</tr>
 +
</tbody>
</table>
</table>
-
<br>
+
<br><br>
-
<br>
+
 
<!--TABLE NEW AND/OR MODIFIED CODING REGION-->
<!--TABLE NEW AND/OR MODIFIED CODING REGION-->
-
<table border="1" width="100%" height="20%" id="Coding">
+
<p class="title1" id="select3">New and/or modified coding region</p>
 +
 
 +
<table style="border-width:1px; border-color:#ccc;width:100%;background-color:#ccc;"
 +
>
 +
<tbody style="background-color:white;">
 +
 
<!--1ere ligne-->
<!--1ere ligne-->
<tr>
<tr>
-
<td><b>Part number/name</b></td>
+
<td style="background-color:#22780F"><p class ="texte" style="color:white; text-align:center; margin: 22px 10px; font-size: 15px;">Part number/name</p></td>
-
<td><b>Natural function of part</b></td>
+
<td style="background-color:#22780F"><p class ="texte" style="color:white; text-align:center; margin: 0 10px; font-size: 15px;">Natural function of part</p></td>
-
<td><b>How did you acquire it?</b></td>
+
<td style="background-color:#22780F"><p class ="texte" style="color:white; text-align:center; margin: 0 10px; font-size: 15px;">How did you acquire it?</p></td>
-
<td><b>How will you use it?</b></td>
+
<td style="background-color:#22780F"><p class ="texte" style="color:white; text-align:center; margin: 0 10px; font-size: 15px;">How will you use it?</p></td>
</tr>
</tr>
<!--2eme ligne-->
<!--2eme ligne-->
<tr>
<tr>
-
<td>RBS (K090505) +  Chimiotaxis + Terminator(B0015)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</p></td>
-
<td>Chimiotaxis code for a chimeric protein (based on McpA (1-34/283-661) from B.sub and VCD_00306(28-315) from V.cholerae)</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">Strong RBS - N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i> - Double terminator</p></td>
-
<td>We order this gene from a synthesis company (Eurofins)</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We ordered this gene from a company (Eurofins)</p></td>
-
<td>This part allows the bacterium to reach the fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part allows the bacterium to reach the fungus</p></td>
-
</tr>
+
-
<!--3eme ligne-->
+
-
<tr>
+
-
<td>RBS (K090505) + binding  + Terminator(B0015)</td>
+
-
<td>"Binding" code for a chimeric binding protein (based on LYCT (1-318) from B.sub and GBPA (324-384) from V.Cholerae) with a linker between.</td>
+
-
<td>We order this gene from a synthesis company (Eurofins)</td>
+
-
<td>This part allows the bacterium to fix the fungus</td>
+
</tr>
</tr>
<!--4eme ligne-->
<!--4eme ligne-->
-
<tr>
+
<tr style=" background-color:#f8f8f8">
-
<td>RBS (K090505) + GAFP1</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</p></td>
-
<td>GAFP1 code for a antifungal. GAFP1 come from arachis hypogaea (Peanut)</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">Strong RBS - Antifungal GAFP-1</p></td>
-
<td>We order this gene from a synthesis company (Eurofins)</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We ordered this gene from a company (Eurofins)</p></td>
-
<td>This part produces a fungicide. By this way, the bacteria is able to kill fungus.</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--5eme ligne-->
<!--5eme ligne-->
<tr>
<tr>
-
<td>RBS (K090505) + D4E1  + Terminator(B0015)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</p></td>
-
<td>D4E1 code for a antifungal. DAE1 is a synthetic gene</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">Strong RBS - Antifungal D4E1 - Double terminator</p></td>
-
<td>We order this gene from a synthesis company (Eurofins)</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We ordered this gene from a company (Eurofins)</p></td>
-
<td>This part produces a fungicide. By this way, the bacteria is able to kill fungus.</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--6eme ligne-->
<!--6eme ligne-->
-
<tr>
+
<tr style=" background-color:#f8f8f8">
-
<td>Promoter(K823003) + (K1364000: RBS (K090505) +  Chimiotaxis + Terminator(B0015))</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</p></td>
-
<td>Promoter Pveg, and Chimiotaxis part</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - Strong RBS - N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i> - Double terminator</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part allows the bacterium to reach the fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part allows the bacterium to reach the fungus</p></td>
</tr>
</tr>
<!--7eme ligne-->
<!--7eme ligne-->
<tr>
<tr>
-
<td>Promoter(K823003) + (K1364001: RBS (K090505) +  Binding + Terminator(B0015))</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</p></td>
-
<td>Promoter Pveg, and binding part</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - RBS consensus - Chitin Binding protein - Double terminator</i></p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part allow the bacterium to fix the fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part allow the bacterium to bind to the fungus</p></td>
</tr>
</tr>
<!--8eme ligne-->
<!--8eme ligne-->
-
<tr>
+
<tr style=" background-color:#f8f8f8">
-
<td>(K1364004: Promoter(K823003) + RBS (K090505) +  Chimiotaxis + Terminator(B0015))+(K1364005: Promoter(K823003) + RBS (K090505) +  Binding + Terminator(B0015))</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364006">BBa_K1364006</p></td>
-
<td>Double expression cassette</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">Double expression cassette</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part allows the bacterium to reach and bind the fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part allows the bacterium to reach and bind to the fungus</p></td>
</tr>
</tr>
<!--9eme ligne-->
<!--9eme ligne-->
<tr>
<tr>
-
<td>Promoteur(K823003) + (K1364002 : RBS (K090505) + GAFP1)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</p></td>
-
<td>Promoter Pveg, and GAFP1 part</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">Strong RBS - Antifungal GAFP-1 - Double terminator</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--10eme ligne-->
<!--10eme ligne-->
-
<tr>
+
<tr style=" background-color:#f8f8f8">
-
<td>(K1364002 : Promoteur(K823003) + RBS (K090505) + GAFP1) + Terminator (B0015)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</p></td>
-
<td>Promoter Pveg, and GAFP1 part with a double terminator</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - Strong RBS - Antifungal GAFP-1 - Double terminator</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--11eme ligne-->
<!--11eme ligne-->
<tr>
<tr>
-
<td>Promoter(K823003) + (K1364003 : RBS (K090505) + D4E1  + Terminator(B0015))</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</p></td>
-
<td>Promoter(K823003) + (K1364003 : RBS (K090505) + D4E1 + Terminator(B0015))</td>
+
<td><p class ="texte" style="text-align:center;">P<sub>veg</sub> - Strong RBS - Antifungal D4E1 - Double Terminator</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--12eme ligne-->
<!--12eme ligne-->
-
<tr>
+
<tr style=" background-color:#f8f8f8">
-
<td>K71364010: Promoter + RBS + EcAMP) + terminator(B0015)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</p></td>
-
<td>Promoter Pveg, RBS SpoVG, Antifungal EcAMP, double terminator</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - RBS SpoVG - Antifungal EcAMP-1</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--13eme ligne-->
<!--13eme ligne-->
<tr>
<tr>
-
<td>K1364002 : RBS  + GAFP1  ) + (K1364003: RBS + D4E1 + terminator)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</p></td>
-
<td>RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - RBS SpoVG - Antifungal EcAMP-1 - Double terminator</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--14eme ligne-->
<!--14eme ligne-->
-
<tr>
+
<tr style=" background-color:#f8f8f8">
-
<td>Promoter(K823003)+(K1364012 : RBS  + GAFP1  +  RBS + D4E1 + terminator)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</p></td>
-
<td>Promoter Pveg, RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - Strong RBS - Antifungal GAFP-1 - Strong RBS - Antifungal D4E1 - Double terminator</p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus.</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
</tr>
</tr>
<!--15eme ligne-->
<!--15eme ligne-->
<tr>
<tr>
-
<td>(K71364010: Promoter + RBS + EcAMP) + (K1364012 : RBS  + GAFP1  +  RBS + D4E1 + terminator)</td>
+
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</p></td>
-
<td>Promoter Pveg,  RBS SpoVG, Antifungal EcAMP, RBS consensus, Antifungal GAFP1, RBS consensus, Antifungal D4E1, double terminator</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - SpoVG RBS -  RFP - Double terminator </p></td>
-
<td>We made it</td>
+
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
-
<td>This part produces a fungicide. By this way, the bacterium is able to kill fungus.</td>
+
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fluorescent protein. Designed to evaluate promotor P<sub>veg</sub> strength.</p></td>
</tr>
</tr>
 +
<!--16eme ligne-->
 +
<tr style=" background-color:#f8f8f8">
 +
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>lepA</sub> - SpoVG RBS - RFP - Double terminator</p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
 +
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fluorescent protein. Designed to evaluate promotor P<sub>lepA</sub> strength.</p></td>
 +
</tr>
 +
<!--17eme ligne-->
 +
<tr>
 +
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>lepA</sub> - SpoVG RBS</p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
 +
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">High efficiency promoter and RBS for translation.</p></td>
 +
</tr>
 +
<!--18eme ligne-->
 +
<tr style=" background-color:#f8f8f8">
 +
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">P<sub>veg</sub> - RBS - Antifungal EcAMP-1 (revised with a stop codon) - Double terminator</p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
 +
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part produces a fungicide (antifungal peptide).</p></td>
 +
</tr>
 +
<!--19eme ligne-->
 +
<tr>
 +
<td><p class ="texte" style="text-align:center;"><a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">Integrative plasmid for <i>Bacillus subtilis</i> (pSB<sub>bs</sub>4E)</i></p></td>
 +
<td><p class ="texte" style="padding-left: 10px; padding-top: 10px; padding-right: 10px; text-align:center;">We made it</p></td>
 +
<td><p class ="texte" style="padding-top: 10px; padding-left: 10px; padding-right: 10px;">This part is an integrative vector for <i>Bacillus subtilis</i>.</p></td>
 +
</tr>
 +
 +
</tbody>
</table>
</table>
<br>
<br>
 +
<p class="title1" id="select4">Team safety</p>
 +
<p class="title2">Safety in INSA Toulouse</p>
 +
<p class="texte">INSA Toulouse is a public school for engineers. The biosafety guidelines are not specific to our institution; the French National regulations for working conditions and for manipulating genetically modified organisms are applied.<br>
 +
The regulation about the workers' prevention against risks resulting from their exposure to pathogenic biological agents (Decree No. 94-352 of 4 May 1994) includes microorganisms, cell cultures and human endoparasites which may cause infections, allergies or toxicity. <br>
 +
This Decree is the French transposition of the Directive 90/679 / EEC and is also transcribed in the Labour Code (Articles L4421-1 R4421-1 to R4427-5.)<br>
 +
The <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000000465273&dateTexte=&categorieLien=id">Decree of the 16th July 2007</a> describes the technical preventive measuresto set up in research laboratories (including containment), education, analysis, anatomy and surgical pathology, autopsy rooms, and industrial and agricultural facilities where workers are likely to be exposed to biological pathogens.<br>
 +
The rules of health, safety, and preventive medicine applied in public services in France (and thus in all public facilities working in scientific and technological domains) are set out in the <a href="http://www.legifrance.gouv.fr/affichTexte.do?cidTexte=LEGITEXT000006063791">Decree No. 82-453</a>. This decree refers to the Labour Code, Public Health Code and Environmental Code.<br>
 +
The <a href="http://legifrance.gouv.fr/affichTexte.do?cidTexte=JORFTEXT000024584737&categorieLien=id">Decree No. 2011-1177</a> is related to the use of genetically modified organisms.<br>
 +
<br>
 +
There is no one in charge of the biological safety at the INSA Toulouse. However there is an organization which includes one prevention advisor and several prevention assistants in every Laboratory. As far as the LISBP (our structure) is concerned, Nathalie Doubrovine is the prevention assistant.<br>
 +
We have already discussed our project with her. She has given us the safety training and advices about how to respond to an imminent danger.<br>
 +
She raised the problem of GMO and the related policy. She advised us of the special procedure for the destruction of GMOs: we cannot wash this kind of organism down the drain.<br>
 +
We were also informed about the potential chemical and microbiological risks.<br>
 +
<br>
 +
The laboratory safety training requirements of the LISBP are detailed into the Rules of Procedures of the LISBP.<br>
 +
The legislation requires employers to inform "all new employees" of the risks that they may encounter as well as ways to protect themselves from those risks. Therefore any newcomer must follow an appropriate training and pass a practical test about safety to ensure its own protection as well as protecting his/her colleagues.<br>
 +
Every person that enters in the LISBP has to make this training whatever its status (researcher, PhD student, trainee, etc.).<br>
 +
The training is divided into two parts. The first one is a training concerning general risk prevention in research laboratories. This one is made individually with the NEO software and with the explanations of the prevention assistant. The second one is a training about the techniques used during the occupation.<br>
 +
<br>
-
<!--ETHIDIUM BROMIDE-->
+
We also had a training to learn how to sort the different sorts of biological waste and how they are destroyed. We also learned that we cannot use autoclave on our own because a specific training is needed. We did not do this specific training and hence did not touch the autoclave. Only our supervisors could start these autoclaves...<br>
-
<p><b>Ethidium bromide</b><br>
+
-
All along our project, we use non-pathogenic strains and non-toxic product, except the EtBr.
+
-
We have a dark room dedicated to the use of EtBr and UV. This room is key-closed and everyone entering into the room must wear gloves, glasses and lab coat. Everything which is in contact directly with something in this room has to stay here.
+
-
Two  specific garbages are dedicated to the gloves and the agarose gels contaminated
+
</p>
</p>
-
<br>
+
<p class="title2">Safety in the lab</p>
 +
<!--CITATION-->
 +
<p class="citation">
 +
"If you don't think it's safe, it probably isn't"</p>
 +
<p class="texte">We have organized our workspace. We have a relaxing room where no biological material should enter. In this room, we can eat and drink (a fridge, a kettle and a coffeemaker are available) but it is also the room where we have our meetings and where we can work on our computers. On the contrary, in the lab, we have to wear protective equipment and respect basic rules.</p>
-
<!--SAFETY PRINCIPLES-->
+
<p class="title3">Personal protective equipment</p>
-
<p><b>Basic safety principles</b><br>
+
<p class="texte">As soon as we manipulate in the lab, we have to wear the following personal protective equipment:</p>
-
All along our project, we use non-pathogenic strains and non-toxic product, except the EtBr.
+
<table>
-
However, we have to apply the basic safety principles into a laboratory room:
+
<tr><td><p class="texte">- A conventional lab coat, closed with long sleeves</p></td>
-
- It is forbidden to smoke in all the INSA's rooms.
+
<td><img width="80px" src="http://2014.igem.org/wiki/images/9/9c/Lab_coat.png"></td></tr>
-
- It is forbidden to drink and eat in the laboratory rooms.
+
<tr><td><p class="texte">- Closed shoes</p></td>
-
- It is compulsory to wear a closed lab coat in cotton.
+
<td><img width="80px" src="http://2014.igem.org/wiki/images/8/82/Shoes.png"></td></tr>
-
- It is compulsory to wear closed shoes.
+
<tr><td><p class="texte">- Gloves</p></td>
-
- Long hair must be tied back
+
<td><img width="80px" src="http://2014.igem.org/wiki/images/3/37/Gloves.png"></td></tr>
-
- Oral pipetting of any substance is prohibited in any laboratory
+
<tr><td><p class="texte">- Glasses if needed (UV exposure, hot water or chemicals manipulation.) </p></td>
-
</p>
+
<td><img width="80px" src="http://2014.igem.org/wiki/images/b/b4/Glasses.png"></td></tr>
 +
</table>
 +
<p class="title3">Basic rules in a lab</p>
 +
<p class="texte">We have to apply the basic safety principles into a laboratory room:<br>
 +
- It is forbidden to smoke in all rooms.<br>
 +
- It is forbidden to drink and eat in the laboratory rooms.<br>
 +
- It is compulsory to wear a closed lab coat in cotton.<br>
 +
- It is compulsory to wear closed shoes.<br>
 +
- Long hair must be tied back.<br>
 +
- Oral pipetting of any substance is prohibited in any laboratory.<br>
<br>
<br>
 +
There are also others precautions when working with biological organisms:<br>
 +
- We need to wash our hands regularly.<br>
 +
- It is compulsory to wear gloves except with the use of an electric burner.<br>
 +
- In some cases (UV light, projection risk), it is compulsory to wear protection glasses.<br>
 +
<br>
 +
Different apparatus are used to work a sterile area.</p>
 +
 +
<p class="title3">Waste</p>
 +
<p class="texte">Different trash containers are available in the lab:<br>
 +
- One for biological waste (yellow). This waste will be autoclaved before being thrown out.<br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/f/f7/Yellow_trash.JPG"><br><br>
 +
- One for common waste (green or orange).<br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/c/c7/Black_garbage.png"><br><br>
 +
- Special waste for chemicals.
 +
 +
<p class="title3">Devices and Material</p>
 +
<p class="texte">We use a lot of different devices, and each one involves a particular risk. Here we described how we use this material safely to reduce risks:</p>
 +
<p class="title4">Chemical storage</p>
 +
<p class="texte">We have three cupboards dedicated to the different kind of chemical products we use:<br>
 +
- Flammable<br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/3/35/Flammable.png"><br><br>
 +
- Acids<br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/3/36/Acids.png"><br><br>
 +
- Bases<br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/7/74/Bases.png"><br><br>
 +
Those cupboards are key-closed.</p>
 +
 +
<p class="title4">Ethidium Bromide</p>
 +
<p class="texte">We have a dark room dedicated to the use of EtBr and UV. This room is key-closed and everyone entering into the room must wear gloves, glasses and lab coat. Everything which is in direct contact with something in this room has to stay here.<br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/7/75/EtBr_room.JPG"><br><br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/4/45/EtBr_door.JPG"><br><br>
 +
Two specific trash cans are dedicated to the gloves or paper and the agarose gels contaminated.<br>
 +
<img width="150px" src="http://2014.igem.org/wiki/images/f/fe/EtBr_garbage.JPG"><br><br></p>
 +
 +
<p class="title4">Biological safety cabinet</p>
 +
<p class="texte">We use a Biological safety cabinet (FASTER – Ultrasafe) to manipulate into a sterile area and thus avoid external contamination by unwanted microorganisms. We clean the bench of the BSC with ethanol before and after each manipulation. We also clean the BSC completely every two weeks. Because we work with fungi, we have to be very careful with the cleanliness of the BSC.</p>
 +
 +
<p class="title4">Electric burner</p>
 +
<p class="texte">We also use electric burners to manipulate into a sterile area. The burning and fire risks are high but weaker than with a Bunsen Burner. We are very cautious when we use electric burners, and we use in preference BSC if it is possible. We do not have to wear gloves and we have to check that the electric burner is turned off after the manipulation.</p>
 +
 +
<p class="title4">Chemical hood</p>
 +
<p class="texte">We use chemical hood in case we have to manipulate dangerous volatile chemical compound.</p>
 +
 +
<p class="title4">Water-bathes</p>
 +
<p class="texte">The water-bathes are used extensively (transformation, digestion, etc.). However, they can be dangerous because of the exposition of boiling or hot water. We use special gloves for protecting us from heat, steam and projections. We verify that we have turned off the water-bathes at the end of the day.</p>
 +
 +
 +
<p class="title2">Safety of our project</p>
<!--CITATION-->
<!--CITATION-->
-
<p align="center"><i>
+
<p class="citation">
-
"Precaution is better than cure"
+
"Precaution is better than cure"</p>
-
</i></p>
+
<p class="texte">Several risks exist when working with microorganisms and manipulating genes. We can identify different classes of risk of our project now: <br>
 +
- risks to the safety and health of team members, or other people working in the lab,<br>
 +
- risks to the safety and health of the general public,<br>
 +
- risks to the environment,<br>
 +
- risks to security through malicious use by individuals, groups, or countries.<br>
 +
<br>
 +
There are also risks in the Future, linked to our project’s growth (new knowledge and methods development).<br>
 +
<p class="title3">Risks to the safety and health of team members, or other people working in the lab</p>
 +
<p class="texte">We work with the <I>B. subtilis </I> and <i>E. coli</i> chassis. These organisms are non-pathogenic. Moreover the used parts are also non-pathogenic (the bio-fungicides produced are harmless for humans).<br>
 +
On one hand, there is a major biological risk because of the manipulation of DNA and RNA of bacterial organisms.<br>
 +
On the other hand the main risk for humans is chemical: we use Ethidium Bromide, hydrochloric acid, sodium hydroxide, solutions from a Miniprep kit, ethanol and bleach.<br>
<br>
<br>
 +
However, we have a black room dedicated to the manipulation of Ethidium Bromide. Everything that touches something in this room cannot get out of the room. The waste has a special treatment.<br>
 +
For the other chemical solutions, we always use gloves and glasses, and if necessary a hood.<br>
 +
Furthermore, there are other risks such as UVs when we analyze an agarose gel but also water-bath and electrical/fire risks.<br>
 +
For protecting us from the UVs, we use adapted glasses.<br>
 +
At the end of every day, the last persons to leave check that everything is ok in the lab (no water-bath stayed on, everything disconnected).</p>
-
<!--SAFETY PRINCIPLES-->
+
<p class="title3">Risks to the safety and health of the general public</p>
-
<p><b>Basic safety principles</b><br>
+
<p class="texte">If biological materials escape from our lab, the risks regarding safety and health of the general public is low because the manipulated parts are harmless. The only risk is the contact with DNA or RNA from a bacterial organism.<br>
-
There is also others precaution when working with biological organisms.
+
Moreover, we have to be cautious with the use of antibiotics and we must not wash them down the drain.</p>
-
- We need to wash our hands regularly.
+
-
- It is compulsory to wear gloves except when working with an electric burner.
+
-
- In some case (UV light, projection risk), it is compulsory to wear protection glasses.
+
-
Indeed, different machine are used to keep a sterile area.
+
-
</p>
+
-
<br>
+
<p class="title3">Risks to the environment</p>
 +
<p class="texte">If biological materials escape from our lab, GMOs can spread in the environment and gene transfer can occur. Moreover, the aim of our bacterial system (called SubtiTree) is to kill a fungus (Ceratocyctis platani). The risk to the environment is high because our system could perturb ecological niche and unbalance the environment of some plants. All our waste is autoclaved to minimize the risk for the environment.<br>
 +
We use three fungicides.<br>
 +
The first one, D4E1, is a synthetic peptide.<br>
 +
The second is EcAMP-1 (BBa_K1162001), an AntiMicrobial Peptide from banyard grass added to the Registry last year by the team Utah State.<br>
 +
The last one is GAFP-1, the Gastrodia AntiFungal Protein.<br>
 +
These fungicides are not authorized yet by the European Union because they are not commercialized.</p>
-
<!--CITATION-->
+
<p class="title3">Risks to security through malicious mis-use by individuals, groups, or countries</p>
-
<p align="center"><i>
+
<p class="texte">If someone steals the biological material it could be dangerous for the environment because our bacterium will produce three bio-fungicides. This could lead to the disturbing of some ecosystems. <br>
-
"If you don't think it's safe, it's probably isn't"
+
However, the fungicides are natural and one of them is used in agriculture. With these considerations, we can assume that the risk is not very high for the chosen parts.</p>
-
</i></p>
+
 
 +
 
 +
<p class="title3">How to reduce these risks?</p>
 +
<p class="texte">We take care of our waste and our biological material.<br>
 +
We have chosen only non-pathogenic species to work on: <i>E. coli</i> and <i>B. subtilis</i>.<br>
 +
We use non-pathogenic fungus to test our SubtiTree system: <i>Aspergillus brasiliensis</i>, <i>Chaetomium globosum</i>, <i>Trichoderma reesei</i> and <i>Aspergillus nidulans</i>.<br>
 +
Indeed the targeted fungus, Ceratocystis platani, is pathogenic. Therefore we avoid working with it.</p>
 +
 
 +
<p class="title3">Risks in the Future</p>
 +
<p class="texte">The system designed could be applied to many fungi. The fungicides used in our project are biological and specifically kill the fungi, and no other eukaryotic cells. <br>
 +
However, a malicious person could replace these fungicides by others which are more dangerous. Indeed our problem is that there is no regulation of the production of fungicides.</p>
 +
<p class="title3">How to reduce these Future risks?</p>
 +
<p class="texte">We have thought about several ways to minimize risks: <br>
 +
- we can build an auxotrophic strain of <I>B. subtilis</I>. The auxotrophy will be for proline, which is readily present in the plant's sap.<br>
 +
- we can build a non-sporulating strain of<I>B. subtilis</I>. Sporulation is a bacterial mechanism used to survive through winter. Thus, our bacterial system sould not stay in the tree after the first year cycle.<br>
 +
- we can build a toxin/antitoxin system to avoid gene transfer between our optimized bacterium and any other wild-type bacteria.</p>
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</div>
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Latest revision as of 03:49, 18 October 2014