Team:Toulouse/Result/parts/Submitted parts

From 2014.igem.org

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<p class="texte">This part is an empty backbone vector for the usage in <i>Bacillus subtilis</i>. It integrates in the thrC locus and can be selected with Erythromycin . It has an Ampicillin resistance for cloning in E.coli. The backbones contains an RFP in the BioBrick site (J04450) to facilitate the cloning in coli.  
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<p class="texte">This part is an empty backbone vector for the usage in <i>Bacillus subtilis</i>. It integrates in the thrC locus and can be selected with Erythromycin . It has an Ampicillin resistance for cloning in E.coli. The backbones contains an RFP in the BioBrick site (J04450) to facilitate the cloning in coli. <br>
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The handling of this type of vector is described <a href="http://2014.igem.org/Team:Toulouse/Notebook/Protocols#select6">here</a>.
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<p class="title4">Design</p>
<p class="title4">Design</p>
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<p class="texte">Backbone</p>
<p class="texte">Backbone</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
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<p class="texte">This part was tested by transforming <i>B. subtilis</i>. Clones obtained were Auxotrophic of Threonine and resistant to Erythromycin.</p>
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<p class="texte">This part was tested by transforming <i>B. subtilis</i>. Clones obtained were Threonine-dependant and resistant to Erythromycin (10µg/ml).</p>
<p class="title4">References</p>
<p class="title4">References</p>
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Revision as of 09:56, 17 October 2014