Team:Toulouse/Result/parts/Submitted parts

From 2014.igem.org

(Difference between revisions)
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<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,  
<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,  
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
+
It is composed of the strong, constitutive promoter of <i>B. subtilis</i>  
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
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by complexing with a sterol present in conodial wall of a varety of fungi.</p>
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
+
<p class="texte">This part is composed of the strong, constitutive promoter of <i>B. subtilis</i>  
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
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<p class="texte">This part is designed for the co-expression of two different peptides  
<p class="texte">This part is designed for the co-expression of two different peptides  
with anti-fungal activities : D4E1 and GAFP-1.  
with anti-fungal activities : D4E1 and GAFP-1.  
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
+
It is composed of the strong, constitutive promoter of <i>B. subtilis</i>  
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)  
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)  
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).</p>
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
-
This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promotor Pveg. This construction has been checked by sequencing and has shown to work also in E. coli
+
This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promoter Pveg. This construction has been checked by sequencing and has shown to work also in E. coli
</p>
</p>
<img src="http://parts.igem.org/wiki/images/6/69/Pveg%2BRFP.jpg">
<img src="http://parts.igem.org/wiki/images/6/69/Pveg%2BRFP.jpg">
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a constitutive promotor Pveg (K823003), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
+
<p class="texte">This part is composed of a constitutive promoter Pveg (K823003), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
-
This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promotor PlepA. This construction has been checked by sequencing and has shown to work also in E. coli
+
This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promoter PlepA. This construction has been checked by sequencing and has shown to work also in E. coli
</p>
</p>
 +
<img src="http://parts.igem.org/wiki/images/1/12/PlepA%2BRFP.jpg">
 +
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a constitutive promotor PlepA (K823002), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
+
<p class="texte">This part is composed of a constitutive promoter PlepA (K823002), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
-
PlepA is a constitutive promotor in Bacillus subtilis (BBa_K823002) coupled with a RBS spoVG (BBa_K143021). To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.  
+
PlepA is a constitutive promoter in Bacillus subtilis (BBa_K823002) coupled with a RBS spoVG (BBa_K143021). To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.  
This construction is also working with E. coli and has been verified by sequencing.
This construction is also working with E. coli and has been verified by sequencing.
</p>
</p>
-
 
+
<img src="http://parts.igem.org/wiki/images/d/da/PlepA%2BSpoVG.jpg">
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Composite</p>
<p class="texte">Composite</p>

Revision as of 09:48, 17 October 2014