Team:Toulouse/Result/parts/Submitted parts

From 2014.igem.org

(Difference between revisions)
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
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This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promotor Pveg. This construction has been checked by sequencing and has shown to work also in E. coli
</p>
</p>
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<img src="http://parts.igem.org/wiki/images/6/69/Pveg%2BRFP.jpg">
<p class="title4">Design</p>
<p class="title4">Design</p>
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<p class="texte">Coming soon! </p>
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<p class="texte">This part is composed of a constitutive promotor Pveg (K823003), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
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<p class="texte">This part was not tested yet</p>
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<p class="texte">This part was tested in both E.coli and B. subtilis. The sequences was verified by sequencing. </p>
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<p class="title4">References</p>
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<p class="texte">Coming soon!</p>
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">Collapse</a></p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
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This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promotor PlepA. This construction has been checked by sequencing and has shown to work also in E. coli
</p>
</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">Coming soon! </p>
+
<p class="texte">This part is composed of a constitutive promotor PlepA (K823002), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
-
<p class="texte">This part was not tested yet</p>
+
<p class="texte">This part was tested in both E.coli and B. subtilis. The sequences was verified by sequencing.</p>
-
<p class="title4">References</p>
+
 
-
<p class="texte">Coming soon!</p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">Collapse</a></p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
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PlepA is a constitutive promotor in Bacillus subtilis (BBa_K823002) coupled with a RBS spoVG (BBa_K143021). To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
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This construction is also working with E. coli and has been verified by sequencing.
</p>
</p>
-
<p class="title4">Design</p>
+
 
-
<p class="texte">Coming soon! </p>
+
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Composite</p>
<p class="texte">Composite</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
-
<p class="texte">This part was not tested yet</p>
+
<p class="texte">This part was checked by sequencing</p>
-
<p class="title4">References</p>
+
 
-
<p class="texte">Coming soon!</p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">Collapse</a></p>

Revision as of 20:48, 16 October 2014