Team:Toulouse/Result/parts/Submitted parts

From 2014.igem.org

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.banniere-content{
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position:absolute;
position:absolute;
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   <div class="fils-ariane" style="width:100%; height:60px; background:#ededed;">
   <div class="fils-ariane" style="width:100%; height:60px; background:#ededed;">
-
   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Results&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Parts</p>  
+
   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Results</a>&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;<a href="https://2014.igem.org/Team:Toulouse/Result/parts">Parts</a>&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Submitted parts</p>  
   </div>
   </div>
Line 148: Line 148:
      
      
-
<p class="title1">Submitted parts</p>
 
-
 
<p class="texte">
<p class="texte">
-
We have deposed 16 new BioBrick parts to the Registry.  
+
We deposited 16 new BioBrick parts in the Registry.  
-
All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.<br>
+
Most of them were cloned into the standard plasmid pSB1C3, tested and sequenced.<br>
 +
All our Biobrick are resumed in <a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Toulouse&Done=1">Parts Sandbox</a>.
<!--à vérifier et confirmer-->
<!--à vérifier et confirmer-->
</p>
</p>
-
<p class="title2">I. Chemotaxis</p>
+
<p class="title1">Chemotaxis</p>
-
 
+
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</a>: N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i></div>
+
-
<div class="thelanguage">
+
 +
<table>
 +
<td valign="top"; align="center";>
 +
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">Strong RBS - N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i> - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte">This part is designed to enable a N-acetylglucosamine  
<p class="texte">This part is designed to enable a N-acetylglucosamine  
-
based chemotaxis in <i>Bacillus subtilis</i>.
+
based chemotaxis in <i>B. subtilis</i>.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
<p class="texte">The encoded protein is a chimera of two proteins:<br>
<p class="texte">The encoded protein is a chimera of two proteins:<br>
-
- the methyl accepting Chemotaxis protein (McpA from <i>Bacillus subtilis</i> which is required for taxis towards glucose.<br>
+
- the methyl accepting Chemotaxis protein (McpA from <i>B. subtilis</i> which is required for taxis towards glucose.<br>
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from <i>Vibrio cholerae</i> (VCD).<br>
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from <i>Vibrio cholerae</i> (VCD).<br>
<br>
<br>
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these domains correspond to the amino acids 1-37 and 282-610.  
these domains correspond to the amino acids 1-37 and 282-610.  
The extracellular domain of VCD is inserted between the intracellular regions of McpA  
The extracellular domain of VCD is inserted between the intracellular regions of McpA  
-
to sense N-acetylglucosamine and corresponds to the amino acids 31-310.<br></p>
+
to sense N-acetylglucosamine and corresponds to the amino acids 31-310.</p>
<img src="https://static.igem.org/mediawiki/2014/a/a2/BBa_K1364000.png">
<img src="https://static.igem.org/mediawiki/2014/a/a2/BBa_K1364000.png">
<p class="title4">Type</p>
<p class="title4">Type</p>
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<p class="texte">Uniprot:<br>
<p class="texte">Uniprot:<br>
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
-
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br></p>
+
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a></p>
<!--à compléter-->
<!--à compléter-->
-
</p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 0); return false">Collapse</a></p>
-
<br>
+
</div></td></table>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">Collapse</a></p>
+
<table>
-
</div>
+
<td valign="top"; align="center";>
-
 
+
<p class="title2";>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a>: P<sub>veg</sub> + N-acetylatedglucosamine based chemotaxis for <i>B. subtilis</i></div>
+
<a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a></p></td>
-
<div class="thelanguage">
+
<td valign="top"; align="center";>
-
+
<div class="technology2">P<sub>veg</sub> - Strong RBS - N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i> - Double terminator</div>
-
<br>
+
<div class="thelanguage2">
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,  
<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,  
-
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
+
D4E1 and for its secretion in <i>B. subtilis</i>.
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
+
It is composed of the strong, constitutive promoter of <i>B. subtilis</i>  
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">BBa_K823003</a>),
-
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of D4E1
the open reading frame of D4E1
-
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)</p>
+
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>)</p>
<img src="https://static.igem.org/mediawiki/2014/3/35/BBa_K1364004.png">
<img src="https://static.igem.org/mediawiki/2014/3/35/BBa_K1364004.png">
<p class="title4">Type</p>
<p class="title4">Type</p>
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- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a></p>
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a></p>
-
 
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 1); return false">Collapse</a></p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">Collapse</a></p>
+
</div></td></table>
-
</div>
+
<br></br>
<br></br>
-
<p class="title2">II. Binding</p>
+
<p class="title1">Binding</p>
-
 
+
<table>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>P<sub>veg</sub> + Chitin Binding Cell Wall protein</div>
+
<td valign="top"; align="center";>
-
<div class="thelanguage">
+
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2"></a>P<sub>veg</sub> - RBS consensus - Chitin Binding protein - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
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<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
</p>
</p>
-
 
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 2); return false">Collapse</a></p>
-
<br>
+
</div></td></table>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">Collapse</a></p>
+
-
</div>
+
<br></br>
<br></br>
-
<p class="title2"> III. Fungicides</p>
+
<p class="title1">Fungicides</p>
<p class="title3">GAFP-1</p>
<p class="title3">GAFP-1</p>
<br>
<br>
-
 
+
<table>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</div>
+
<td valign="top"; align="center";>
-
<div class="thelanguage">
+
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2"> Strong RBS - Antifungal GAFP-1</div>
 +
<div class="thelanguage2">
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
-
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
  originating from the Asiatic orchid <i>Gastrodia elata</i>, a traditional Chinese medicinal herb cultured for thousands of years.
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) and
+
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>) and
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
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<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373</p>
-
<br>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 3); return false">Collapse</a></p>
-
 
+
</div></td></table>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">Collapse</a></p>
+
-
</div>
+
-
 
+
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</div>
+
-
<div class="thelanguage">
+
-
 
+
 +
<table>
 +
<td valign="top"; align="center";>
 +
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">Strong RBS - Antifungal GAFP-1 - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
-
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
  originating from the Asiatic orchid <i>Gastrodia elata</i>, a traditional Chinese medicinal herb cultured for thousands of years.
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
The codon optimization was made thanks to the DNA 2.0 software program.</p>
The codon optimization was made thanks to the DNA 2.0 software program.</p>
Line 296: Line 305:
<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373</p>
-
<br>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 4); return false">Collapse</a></p>
-
 
+
</div></td></table>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">Collapse</a></p>
+
-
</div>
+
-
 
+
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</div>
+
-
<div class="thelanguage">
+
 +
<table>
 +
<td valign="top";align="center";>
 +
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2"> P<sub>veg</sub> - Strong RBS - Antifungal GAFP-1 - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin
<p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin
-
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
  originating from the Asiatic orchid <i>Gastrodia elata</i>, a traditional Chinese medicinal herb cultured for thousands of years.
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">BBa_K823003</a>),
-
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
optimized for its expression and its secretion in <i>B. subtilis</i>.
The codon optimization was made thanks to the DNA 2.0 software program.</p>
The codon optimization was made thanks to the DNA 2.0 software program.</p>
-
<img src="https://static.igem.org/mediawiki/2014/6/67/BBa_K1364008.png">
+
<img src="https://static.igem.org/mediawiki/parts/4/47/1364008a.jpg">
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Generator</p>
<p class="texte">Generator</p>
Line 322: Line 333:
<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
</p>
</p>
-
 
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 5); return false">Collapse</a></p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">Collapse</a></p>
+
</div></td></table>
-
</div>
+
<br></br>
<br></br>
Line 333: Line 343:
<br>
<br>
-
 
+
<table>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</div>
+
<td valign="top"; align="center";>
-
<div class="thelanguage">
+
<p class="title2";>
-
 
+
<a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a></p></td>
-
 
+
<td valign="top"; align="center";>
 +
<div class="technology2">Strong RBS - Antifungal D4E1 - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids  
<p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids  
which has shown to have antifungal activities  
which has shown to have antifungal activities  
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of D4E1 and  
the open reading frame of D4E1 and  
-
a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)
+
a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>)
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
This part was optimized for the expression and its secretion in <i>B. subtilis </i>
thanks to the DNA 2.0 software program.</p>
thanks to the DNA 2.0 software program.</p>
<img src="https://static.igem.org/mediawiki/2014/b/bb/BBa_K1364003.png">
<img src="https://static.igem.org/mediawiki/2014/b/bb/BBa_K1364003.png">
Line 356: Line 368:
<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 6); return false">Collapse</a></p>
 +
</div></td></table>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">Collapse</a></p>
+
<table>
-
</div>
+
<td valign="top"; align="center";>
-
 
+
<p class="title2";>
-
 
+
<a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a></p></td>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</div>
+
<td valign="top"; align="center";>
-
<div class="thelanguage">
+
<div class="technology2">P<sub>veg</sub> - Strong RBS - Antifungal D4E1 - Double Terminator</div>
-
 
+
<div class="thelanguage2">
<p class="texte">
<p class="texte">
D4E1 is a linear synthetic peptide of 17 amino acids  
D4E1 is a linear synthetic peptide of 17 amino acids  
Line 369: Line 383:
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
+
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i>  
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">BBa_K823003</a>),
-
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of D4E1 and  
the open reading frame of D4E1 and  
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
+
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>).
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
This part was optimized for the expression and its secretion in <i>B.subtilis </i>
thanks to the DNA 2.0 software program.</p>
thanks to the DNA 2.0 software program.</p>
<img src="https://static.igem.org/mediawiki/2014/4/47/BBa_K1364009.png">
<img src="https://static.igem.org/mediawiki/2014/4/47/BBa_K1364009.png">
Line 385: Line 399:
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">Collapse</a></p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 7); return false">Collapse</a></p>
-
</div>
+
</div></td></table>
<br></br>
<br></br>
Line 392: Line 406:
<p class="title3">EcAMP-1</p>
<p class="title3">EcAMP-1</p>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</div>
+
<table>
-
<div class="thelanguage">
+
<td valign="top"; align="center";>
-
 
+
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">P<sub>veg</sub> - RBS SpoVG - Antifungal EcAMP-1</div>
 +
<div class="thelanguage2">
<p class="texte">
<p class="texte">
EcAMP-1 is an antimicrobial peptide of 37 amino acids  
EcAMP-1 is an antimicrobial peptide of 37 amino acids  
Line 404: Line 422:
<p class="title4">Design</p>
<p class="title4">Design</p>
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
-
strong RBS for <i>B. subtilis</i>
+
strong RBS for <i>Bacillus subtilis</i>
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>) and
+
(<a href="http://parts.igem.org/Part:BBa_K733013">BBa_K733013</a>) and
the open reading frame of EcAMP-1.<br>
the open reading frame of EcAMP-1.<br>
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
Line 415: Line 433:
<p class="texte">This part is not tested yet.</p>
<p class="texte">This part is not tested yet.</p>
<p class="title4">References</p>
<p class="title4">References</p>
-
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
+
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (<i>Echinochloa crus-galli</i>)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">Collapse</a></p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 8); return false">Collapse</a></p>
-
</div>
+
</div></td></table>
-
 
+
<table>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</div>
+
<td valign="top"; align="center";>
-
<div class="thelanguage">
+
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2"> P<sub>veg</sub> - RBS SpoVG - Antifungal EcAMP-1 - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte" id="select10">
<p class="texte" id="select10">
Line 433: Line 455:
<p class="title4">Design</p>
<p class="title4">Design</p>
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
-
strong RBS for <i>B. subtilis</i>
+
strong RBS for <i>Bacillus subtilis</i>
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
+
(<a href="http://parts.igem.org/Part:BBa_K733013">BBa_K733013</a>),
the open reading frame of EcAMP-1 and  
the open reading frame of EcAMP-1 and  
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
+
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>).<br>
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
The EcAMP-1 part was codon optimized for <i>Escherichia coli</i> by the iGEM Utah State team
and thanks to the Life Technologies GeneArt software program. </p>
and thanks to the Life Technologies GeneArt software program. </p>
<img src="https://static.igem.org/mediawiki/2014/7/74/BBa_K1364011.png">
<img src="https://static.igem.org/mediawiki/2014/7/74/BBa_K1364011.png">
Line 445: Line 467:
<p class="texte">This part is not tested yet.</p>
<p class="texte">This part is not tested yet.</p>
<p class="title4">References</p>
<p class="title4">References</p>
-
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
+
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (<i>Echinochloa crus-galli</i>)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">Collapse</a></p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 9); return false">Collapse</a></p>
-
</div>
+
</div></td></table>
-
 
+
<table>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>: P<sub>veg</sub> - RBS - Antifungal EcAMP (revised with a stop codon) - Double terminator</div>
+
<td valign="top"; align="center";>
-
<div class="thelanguage">
+
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">P<sub>veg</sub> - RBS - Antifungal EcAMP-1 (revised with a stop codon) - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte">EcAMP-1 is an antimicrobial peptide of 37 amino acids  
<p class="texte">EcAMP-1 is an antimicrobial peptide of 37 amino acids  
Line 464: Line 490:
<p class="title4">Design</p>
<p class="title4">Design</p>
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
-
strong RBS for <i>B. subtilis</i>
+
strong RBS for <i>Bacillus subtilis</i>
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
+
(<a href="http://parts.igem.org/Part:BBa_K733013">BBa_K733013</a>),
<b>the revised open reading frame of EcAMP-1 (with a stop codon) </b> and  
<b>the revised open reading frame of EcAMP-1 (with a stop codon) </b> and  
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
+
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>).<br>
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
The EcAMP-1 part was codon optimized for <i>Escherichia coli</i> by the iGEM Utah State team
and thanks to the Life Technologies GeneArt software program. </p>
and thanks to the Life Technologies GeneArt software program. </p>
<img src="https://static.igem.org/mediawiki/2014/7/74/BBa_K1364011.png">
<img src="https://static.igem.org/mediawiki/2014/7/74/BBa_K1364011.png">
Line 476: Line 502:
<p class="texte">This part was not tested yet</p>
<p class="texte">This part was not tested yet</p>
<p class="title4">References</p>
<p class="title4">References</p>
-
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
+
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (<i>Echinochloa crus-galli</i>)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">Collapse</a></p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 13); return false">Collapse</a></p>
-
</div>
+
</div></td></table>
<br></br>
<br></br>
Line 485: Line 511:
<p class="title3">Fungicide operons</p>
<p class="title3">Fungicide operons</p>
   
   
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungals GAFP-1 and D4E1 - Double terminator</div>
+
<table>
-
<div class="thelanguage">
+
<td valign="top"; align="center";>
-
 
+
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">P<sub>veg</sub> - Strong RBS - Antifungal GAFP-1 - Strong RBS - Antifungal  D4E1 - Double terminator</div>
 +
<div class="thelanguage2">
<p class="texte">
<p class="texte">
<p class="title4">Design</p>
<p class="title4">Design</p>
<p class="texte">This part is designed for the co-expression of two different peptides  
<p class="texte">This part is designed for the co-expression of two different peptides  
with anti-fungal activities : D4E1 and GAFP-1.  
with anti-fungal activities : D4E1 and GAFP-1.  
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
+
It is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i>  
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)  
+
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">BBa_K823003</a>)  
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).</p>
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).</p>
<img src="https://static.igem.org/mediawiki/2014/4/40/BBa_K1364013.png">
<img src="https://static.igem.org/mediawiki/2014/4/40/BBa_K1364013.png">
Line 503: Line 533:
See <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a> and <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>.</p>
See <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a> and <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>.</p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">Collapse</a></p>
+
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 10); return false">Collapse</a></p>
-
</div>
+
</div></td></table>
<br></br>
<br></br>
-
<p class="title2">Basic tools</p>
+
<p class="title1">Basic tools</p>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</div>
+
<table>
-
<div class="thelanguage">
+
<td valign="top"; align="center";>
 +
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">P<sub>veg</sub> - SpoVG RBS -  RFP - Double terminator </div>
 +
<div class="thelanguage2">
<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
-
This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promotor Pveg. This construction has been checked by sequencing and has shown to work also in E. coli
+
This part is designed to enable the expression of a Red Fluorescent Protein in <i>Bacillus subtilis</i> under the control of a constitutive promoter P<sub>veg</sub>. This construction has been checked by sequencing and has shown to work also in <i>Escherichia coli</i>
</p>
</p>
<img src="https://static.igem.org/mediawiki/parts/6/69/Pveg%2BRFP.jpg">
<img src="https://static.igem.org/mediawiki/parts/6/69/Pveg%2BRFP.jpg">
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a constitutive promotor Pveg (K823003), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
+
<p class="texte">This part is composed of a constitutive promoter P<sub>veg</sub> (BBa_K823003), spoVG RBS (BBa_KK143021), the coding sequence of the RFP (E1010 from BBa_K606013) and a double terminator (BBa_KB0015) </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
-
<p class="texte">This part was tested in both E.coli and B. subtilis. The sequences was verified by sequencing. </p>
+
<p class="texte">This part was tested in both <i>E. coli</i> and <i>B. subtilis</i>. The sequences was verified by sequencing. </p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">Collapse</a></p>
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 10); return false">Collapse</a></p>
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</div>
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</div></td></table>
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</div>
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<table>
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<div class="thelanguage">
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<td valign="top"; align="center";>
 +
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2"> P<sub>lepA</sub> - SpoVG RBS - RFP - Double terminator </div>
 +
<div class="thelanguage2">
<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
-
This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promotor PlepA. This construction has been checked by sequencing and has shown to work also in E. coli
+
This part is designed to enable the expression of a Red Fluorescent Protein in <i>Bacillus subtilis</i> under the control of a constitutive promoter P<sub>lepA</sub>. This construction has been checked by sequencing and has shown to work also in <i>Escherichia coli</i>
</p>
</p>
 +
<img src="https://static.igem.org/mediawiki/parts/1/12/PlepA%2BRFP.jpg">
 +
<p class="title4">Design</p>
<p class="title4">Design</p>
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<p class="texte">This part is composed of a constitutive promotor PlepA (K823002), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015) </p>
+
<p class="texte">This part is composed of a constitutive promoter P<sub>lepA</sub> (BBa_K823002), spoVG RBS (BBa_K143021), the coding sequence of the RFP (E1010 from BBa_K606013) and a double terminator (BBa_B0015) </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
-
<p class="texte">This part was tested in both E.coli and B. subtilis. The sequences was verified by sequencing.</p>
+
<p class="texte">This part was tested in both <i>E. coli</i> and <i>B. subtilis</i>. The sequences was verified by sequencing.</p>
-
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">Collapse</a></p>
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 11); return false">Collapse</a></p>
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</div>
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</div></td></table>
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</div>
+
<table>
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<div class="thelanguage">
+
<td valign="top"; align="center";>
 +
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">P<sub>lepA</sub> - SpoVG RBS</div>
 +
<div class="thelanguage2">
<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
-
PlepA is a constitutive promotor in Bacillus subtilis (BBa_K823002) coupled with a RBS spoVG (BBa_K143021). To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.  
+
P<sub>lepA</sub> is a constitutive promoter in <i>Bacillus subtilis</i> (BBa_K823002) coupled with a RBS spoVG (BBa_K143021). To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.  
-
This construction is also working with E. coli and has been verified by sequencing.
+
This construction is also working with <i>Escherichia coli</i> and has been verified by sequencing.
</p>
</p>
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<img src="https://static.igem.org/mediawiki/parts/d/da/PlepA%2BSpoVG.jpg">
<p class="title4">Type</p>
<p class="title4">Type</p>
<p class="texte">Composite</p>
<p class="texte">Composite</p>
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<p class="texte">This part was checked by sequencing</p>
<p class="texte">This part was checked by sequencing</p>
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 12); return false">Collapse</a></p>
 +
</div></td></table>
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">Collapse</a></p>
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<table>
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</div>
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<td valign="top"; align="center";>
 +
<p class="title2";>
 +
<a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a></p></td>
 +
<td valign="top"; align="center";>
 +
<div class="technology2">Integrative plasmid for <i>Bacillus subtilis</i> (pSB<sub>bs</sub>4E)</div>
 +
<div class="thelanguage2">
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<p class="texte">This part is an empty backbone vector for the usage in <i>Bacillus subtilis</i>. It is on biobrick version of pKL190 which integrates in the thrC locus and can be selected with Erythromycin . It has an Ampicillin resistance for cloning in <i>Escherichia coli</i>. The backbone contains an RFP in the BioBrick site (BBa_J04450) to facilitate the cloning in <i>E.coli</i>. <br>
-
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></div>
+
The handling of this type of vector is described <a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols#select6">here</a>.
-
<div class="thelanguage">
+
-
 
+
-
<p class="texte"><!--Compléter-->
+
</p>
</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">Coming soon! </p>
+
<p class="texte">We digested pKL190 plasmid and PCR products of BBa_J04450 with BamHI and EcoRI. We proceded to ligation.</p>
 +
<img src="https://static.igem.org/mediawiki/2014/archive/6/6d/20141017182537!PKL190_map.png">
<p class="title4">Type</p>
<p class="title4">Type</p>
-
<p class="texte">Composite</p>
+
<p class="texte">Backbone</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
-
<p class="texte">This part was not tested yet</p>
+
<p class="texte">This part was tested by transforming <i>B. subtilis</i>. Clones obtained were Threonine-dependant and resistant to Erythromycin (10µg/ml).</p>
<p class="title4">References</p>
<p class="title4">References</p>
-
<p class="texte">Coming soon!</p>
+
<p class="texte">
-
 
+
R. Bernard, K.A. Marquis, and D.Z. Rudner. <b>Nucleoid occlusion prevents cell division during replication fork arrest in <i>Bacillus subtilis</i></b>Mol Microbiol. Nov 2010; 78(4): 866–882.</p>
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">Collapse</a></p>
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 13); return false">Collapse</a></p>
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</div>
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</div></td></table>
<br></br>
<br></br>
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   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
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     <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Experimental results
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     <a href="https://2014.igem.org/Team:Toulouse/Modelling" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Modeling
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
     </a>  
     </a>  
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     <a href="https://2014.igem.org/Team:Toulouse/Result/achievement" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
     <a href="https://2014.igem.org/Team:Toulouse/Result/achievement" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
-
color:#666; font-size:18px;">Achievement</br>
+
color:#666; font-size:18px;">Achievements</br>
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
     </a>
     </a>

Latest revision as of 03:56, 18 October 2014