Team:Toulouse/Result/parts/Submitted parts

From 2014.igem.org

(Difference between revisions)
Line 193: Line 193:
<a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a></p></td>
<a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a></p></td>
<td valign="top"; align="center";>
<td valign="top"; align="center";>
-
<div class="technology2">P<sub>veg</sub> + N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i></div>
+
<div class="technology2">P<sub>veg</sub> - N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i></div>
<div class="thelanguage2">
<div class="thelanguage2">
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
Line 200: Line 200:
D4E1 and for its secretion in <i>B. subtilis</i>.
D4E1 and for its secretion in <i>B. subtilis</i>.
It is composed of the strong, constitutive promoter of <i>B. subtilis</i>  
It is composed of the strong, constitutive promoter of <i>B. subtilis</i>  
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">BBa_K823003</a>),
-
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of D4E1
the open reading frame of D4E1
-
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)</p>
+
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>)</p>
<img src="https://static.igem.org/mediawiki/2014/3/35/BBa_K1364004.png">
<img src="https://static.igem.org/mediawiki/2014/3/35/BBa_K1364004.png">
<p class="title4">Type</p>
<p class="title4">Type</p>
Line 225: Line 225:
<a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a></p></td>
<a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a></p></td>
<td valign="top"; align="center";>
<td valign="top"; align="center";>
-
<div class="technology2"></a>P<sub>veg</sub> + Chitin Binding Cell Wall protein</div>
+
<div class="technology2"></a>P<sub>veg</sub> - Chitin Binding Cell Wall protein</div>
<div class="thelanguage2">
<div class="thelanguage2">
Line 244: Line 244:
<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
</p>
</p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 2); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 2); return false">Collapse</a></p>
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  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) and
+
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>) and
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
Line 278: Line 278:
<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373</p>
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373</p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 3); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 3); return false">Collapse</a></p>
</div></td></table>
</div></td></table>
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<a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a></p></td>
<a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a></p></td>
<td valign="top"; align="center";>
<td valign="top"; align="center";>
-
<div class="technology2">RBS + Antifungal GAFP-1 + Double terminator</div>
+
<div class="technology2">RBS - Antifungal GAFP-1 + Double terminator</div>
<div class="thelanguage2">
<div class="thelanguage2">
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
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  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
The codon optimization was made thanks to the DNA 2.0 software program.</p>
The codon optimization was made thanks to the DNA 2.0 software program.</p>
Line 305: Line 305:
<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373</p>
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373</p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 4); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 4); return false">Collapse</a></p>
</div></td></table>
</div></td></table>
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  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">BBa_K823003</a>),
-
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
Line 333: Line 333:
<p class="title4">References</p>
<p class="title4">References</p>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
+
- The <i>Vibrio cholerae</i> Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
</p>
</p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 5); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 5); return false">Collapse</a></p>
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by complexing with a sterol present in conodial wall of a varety of fungi.</p>
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
<p class="title4">Design</p>
<p class="title4">Design</p>
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of D4E1 and  
the open reading frame of D4E1 and  
-
a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)
+
a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>)
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
This part was optimized for the expression and its secretion in <i>B. subtilis </i>
This part was optimized for the expression and its secretion in <i>B. subtilis </i>
Line 384: Line 384:
<p class="title4">Design</p>
<p class="title4">Design</p>
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i>  
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i>  
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">BBa_K823003</a>),
-
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">BBa_K780002</a>),
the open reading frame of D4E1 and  
the open reading frame of D4E1 and  
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
+
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>).
This part was optimized for the expression and its secretion in <i>B.subtilis </i>
This part was optimized for the expression and its secretion in <i>B.subtilis </i>
thanks to the DNA 2.0 software program.</p>
thanks to the DNA 2.0 software program.</p>
Line 423: Line 423:
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
strong RBS for <i>Bacillus subtilis</i>
strong RBS for <i>Bacillus subtilis</i>
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>) and
+
(<a href="http://parts.igem.org/Part:BBa_K733013">BBa_K733013</a>) and
the open reading frame of EcAMP-1.<br>
the open reading frame of EcAMP-1.<br>
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
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<p class="texte">This part is not tested yet.</p>
<p class="texte">This part is not tested yet.</p>
<p class="title4">References</p>
<p class="title4">References</p>
-
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
+
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (<i>Echinochloa crus-galli</i>)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 8); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 8); return false">Collapse</a></p>
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<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
strong RBS for <i>Bacillus subtilis</i>
strong RBS for <i>Bacillus subtilis</i>
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
+
(<a href="http://parts.igem.org/Part:BBa_K733013">BBa_K733013</a>),
the open reading frame of EcAMP-1 and  
the open reading frame of EcAMP-1 and  
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
+
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>).<br>
The EcAMP-1 part was codon optimized for <i>Escherichia coli</i> by the iGEM Utah State team
The EcAMP-1 part was codon optimized for <i>Escherichia coli</i> by the iGEM Utah State team
and thanks to the Life Technologies GeneArt software program. </p>
and thanks to the Life Technologies GeneArt software program. </p>
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<p class="texte">This part is not tested yet.</p>
<p class="texte">This part is not tested yet.</p>
<p class="title4">References</p>
<p class="title4">References</p>
-
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
+
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (<i>Echinochloa crus-galli</i>)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 9); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 9); return false">Collapse</a></p>
Line 491: Line 491:
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
strong RBS for <i>Bacillus subtilis</i>
strong RBS for <i>Bacillus subtilis</i>
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
+
(<a href="http://parts.igem.org/Part:BBa_K733013">BBa_K733013</a>),
<b>the revised open reading frame of EcAMP-1 (with a stop codon) </b> and  
<b>the revised open reading frame of EcAMP-1 (with a stop codon) </b> and  
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
+
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>).<br>
The EcAMP-1 part was codon optimized for <i>Escherichia coli</i> by the iGEM Utah State team
The EcAMP-1 part was codon optimized for <i>Escherichia coli</i> by the iGEM Utah State team
and thanks to the Life Technologies GeneArt software program. </p>
and thanks to the Life Technologies GeneArt software program. </p>
Line 502: Line 502:
<p class="texte">This part was not tested yet</p>
<p class="texte">This part was not tested yet</p>
<p class="title4">References</p>
<p class="title4">References</p>
-
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
+
<p class="texte">Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (<i>Echinochloa crus-galli</i>)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153</p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 13); return false">Collapse</a></p>
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology2', 13); return false">Collapse</a></p>
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<a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a></p></td>
<a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a></p></td>
<td valign="top"; align="center";>
<td valign="top"; align="center";>
-
<div class="technology2">P<sub>veg</sub> + RFP</div>
+
<div class="technology2">P<sub>veg</sub> - RFP</div>
<div class="thelanguage2">
<div class="thelanguage2">
Line 557: Line 557:
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
-
<p class="texte">This part was tested in both <i>E.coli</i> and <i>B. subtilis</i>. The sequences was verified by sequencing. </p>
+
<p class="texte">This part was tested in both <i>E. coli</i> and <i>B. subtilis</i>. The sequences was verified by sequencing. </p>
Line 568: Line 568:
<a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a></p></td>
<a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a></p></td>
<td valign="top"; align="center";>
<td valign="top"; align="center";>
-
<div class="technology2"> P<sub>lepA</sub> + RFP</div>
+
<div class="technology2"> P<sub>lepA</sub> - RFP</div>
<div class="thelanguage2">
<div class="thelanguage2">
Line 581: Line 581:
<p class="texte">Reporter</p>
<p class="texte">Reporter</p>
<p class="title4">Tests</p>
<p class="title4">Tests</p>
-
<p class="texte">This part was tested in both <i>E.coli</i> and <i>B. subtilis</i>. The sequences was verified by sequencing.</p>
+
<p class="texte">This part was tested in both <i>E. coli</i> and <i>B. subtilis</i>. The sequences was verified by sequencing.</p>
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<a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a></p></td>
<a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a></p></td>
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<td valign="top"; align="center";>
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<div class="technology2">P<sub>lepA</sub> + RBS SpoVG</div>
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<div class="technology2">P<sub>lepA</sub> - RBS SpoVG</div>
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<div class="thelanguage2">
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<div class="thelanguage2">
<div class="thelanguage2">
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<p class="texte">This part is an empty backbone vector for the usage in <i>Bacillus subtilis</i>. It integrates in the thrC locus and can be selected with Erythromycin . It has an Ampicillin resistance for cloning in <i>Escherichia coli</i>. The backbones contains an RFP in the BioBrick site (BBa_KJ04450) to facilitate the cloning in <i>E.coli</i>. <br>
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<p class="texte">This part is an empty backbone vector for the usage in <i>Bacillus subtilis</i>. It is on biobrick version of pKL190 which integrates in the thrC locus and can be selected with Erythromycin . It has an Ampicillin resistance for cloning in <i>Escherichia coli</i>. The backbone contains an RFP in the BioBrick site (BBa_J04450) to facilitate the cloning in <i>E.coli</i>. <br>
The handling of this type of vector is described <a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols#select6">here</a>.
The handling of this type of vector is described <a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols#select6">here</a>.
   
   

Revision as of 18:21, 17 October 2014