Team:Toulouse/Result/parts/Submitted parts

From 2014.igem.org

(Difference between revisions)
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<p class="title2">II. Binding</p>
<p class="title2">II. Binding</p>
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<!--K1364001 : RBS + Binding + Terminator-->
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>:  P<sub>veg</sub> + Chitin Binding Cell Wall protein</div>
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<p class="title3" id="select3">
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<div class="thelanguage">
-
- <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>:  P<sub>veg</sub> + Chitin Binding Cell Wall protein</p>
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<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
<p class="title4">Design</p>
<p class="title4">Design</p>
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<br>
<br>
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<br></br>
<p class="title2"> III. Fungicides</p>
<p class="title2"> III. Fungicides</p>
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<br>
<br>
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<!--K1364002 : RBS + GAFP-1-->
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</div>
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<p class="title3" id="select4">
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<div class="thelanguage">
-
- <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</p>
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<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
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<br>
<br>
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<!--K1364007 : RBS + GAFP-1 + Terminator-->
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<p class="title3" id="select5">
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</div>
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- <a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</p>
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 +
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</div>
 +
<div class="thelanguage">
 +
 
 +
 
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
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<br>
<br>
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<!--K13640008 : Pveg + RBS + GAFP-1 + Terminator-->
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<p class="title3" id="select6">
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</div>
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- <a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p>
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 +
<div class="technology">a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</div>
 +
<div class="thelanguage">
 +
 
<p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin
<p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
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</p>
</p>
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<br></br>
<p class="title3">D4E1</p>
<p class="title3">D4E1</p>
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<br>
<br>
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<!--K1364003 : RBS + D4E1 + Terminator-->
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</div>
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<p class="title3" id="select7">
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<div class="thelanguage">
-
- <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</p>
+
 
 +
 
<p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids  
<p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids  
which has shown to have antifungal activities  
which has shown to have antifungal activities  
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- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
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</div>
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<br>
 
-
<!--K1364009 : Pveg + RBS + D4E1 + Terminator-->
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</div>
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<p class="title3" id="select8">
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<div class="thelanguage">
-
- <a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p>
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<p class="texte">
<p class="texte">
D4E1 is a linear synthetic peptide of 17 amino acids  
D4E1 is a linear synthetic peptide of 17 amino acids  
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<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
-
<br>
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<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">Collapse</a></p>
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<br></br>
<p class="title3">EcAMP-1</p>
<p class="title3">EcAMP-1</p>
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<!--K1364010 : Pveg RBS + EcAMP -->
+
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</div>
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<p class="title3" id="select9">
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<div class="thelanguage">
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- <a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</p>
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<p class="texte">
<p class="texte">
EcAMP-1 is an antimicrobial peptide of 37 amino acids  
EcAMP-1 is an antimicrobial peptide of 37 amino acids  
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<p class="texte"><!--à compléter : demander à emeline--></p>
<p class="texte"><!--à compléter : demander à emeline--></p>
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</div>
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</div>
 +
<div class="thelanguage">
-
<!--K1364011 : Pveg RBS + EcAMP + Double term-->
 
-
<p class="title3">
 
-
- <a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
 
<p class="texte" id="select10">
<p class="texte" id="select10">
EcAMP-1 is an antimicrobial peptide of 37 amino acids  
EcAMP-1 is an antimicrobial peptide of 37 amino acids  
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<p class="texte"><!--à compléter : demander à emeline--></p>
<p class="texte"><!--à compléter : demander à emeline--></p>
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</div>
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<br></br>
<p class="title3">Fungicide operons</p>
<p class="title3">Fungicide operons</p>
   
   
-
<!--K1364013 : Pveg + RBS + GAFP + RBS + D4E1 + Terminator-->
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</div>
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<p class="title3" id="select11">
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<div class="thelanguage">
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- <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
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<p class="texte">
<p class="texte">
<p class="title4">Design</p>
<p class="title4">Design</p>
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<p class="title4">References</p>
<p class="title4">References</p>
See <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a> and <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>.</p>
See <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a> and <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>.</p>
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<br>
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<br></br>
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<p class="title2">Basic tools</p>
<p class="title2">Basic tools</p>
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<!--K1364015-->
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</div>
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<p class="title3" id="select12">
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<div class="thelanguage">
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- <a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="texte">Coming soon!</p>
<p class="texte">Coming soon!</p>
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</div>
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 +
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</div>
 +
<div class="thelanguage">
-
<!--K1364016-->
 
-
<p class="title3" id="select13">
 
-
- <a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</p>
 
<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="texte">Coming soon!</p>
<p class="texte">Coming soon!</p>
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</div>
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<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</div>
 +
<div class="thelanguage">
-
<!--K1364017-->
 
-
<p class="title3" id="select14">
 
-
- <a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</p>
 
<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="texte">Coming soon!</p>
<p class="texte">Coming soon!</p>
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</div>
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<div class="technology">***</div>
 +
<div class="thelanguage">
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<!--K1364019-->
 
<p class="title3" id="select15">
<p class="title3" id="select15">
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- <a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>: P<sub>veg</sub> + RBS + Antifungal EcAMP + Double terminator</p>
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- </p>
<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="texte">Coming soon!</p>
<p class="texte">Coming soon!</p>
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</div>
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 +
<div class="technology"><a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></div>
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<div class="thelanguage">
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<!--K1364021-->
 
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<p class="title3" id="select16">
 
-
- <a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></p>
 
<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="texte">Coming soon!</p>
<p class="texte">Coming soon!</p>
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Revision as of 20:35, 15 October 2014

           
        logo-igem            

Parts

What did we send to the Registry?

Results   >   Parts

Submitted parts

We have deposed 16 new BioBrick parts to the Registry. All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.

Collapse all | Expand all

I. Chemotaxis

BBa_K1364000: N-acetylglucosamine based chemotaxis for Bacillus subtilis

This part is designed to enable a N-acetylglucosamine based chemotaxis in Bacillus subtilis.

Design

The encoded protein is a chimera of two proteins:
- the methyl accepting Chemotaxis protein (McpA from Bacillus subtilis which is required for taxis towards glucose.
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from Vibrio cholerae (VCD).

The chimeric protein contains the intracellular domains of McpA from Bacillus subtilis to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids 31-310.

Type

Translation unit

Tests

This part is not tested yet.

References

Uniprot:
- McpA
- N-acetylglucosamine regulated methyl-accepting chemotaxis protein


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BBa_K1364004: Pveg + N-acetylatedglucosamine based chemotaxis for B. subtilis

This part is designed to enable a N-acetylglucosamine based chemotaxis in Bacillus subtilis.

Design

This expression cassette is designed for the expression of an antifungal peptide, D4E1 and for its secretion in Bacillus subtilis. It is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003), strong RBS for B. subtilis (K780002), the open reading frame of D4E1 and a double terminator (B0015)

Type

Generator

Tests

This part was not tested yet.

References

Uniprot:
- McpA
- N-acetylglucosamine regulated methyl-accepting chemotaxis protein

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II. Binding

BBa_K1364005: Pveg + Chitin Binding Cell Wall protein

This part is designed to enable the binding of Bacillus subtilis to the fungi wall made of chitin.

Design

This part is composed of a Cell Wall Binding (CWB) sequence and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.

The CWB domain has been extracted from LytC (BBa_K316030) of the iGEM 2010 Imperial College team.
The CBD is composed of the Domain 4 of the GbpA protein of Vibrio cholerae, a protein reported to mediate bacterial attachment to chitin. It has been shown that the Domain 4 was essential to bind chitin.

Type

Generator

Tests

This part was tested with chitin beads. (See Binding module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373


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III. Fungicides

GAFP-1


BBa_K1364002: RBS - Antifungal GAFP-1

The Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.

Design

This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.

Type

Translational unit

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373


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BBa_K1364007: RBS + Antifungal GAFP-1 + Double terminator

The Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.

Design

This part is composed of a Strong RBS (K780002), the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) and a double terminator B0015. optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.

Type

Composite part

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373


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a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008: Pveg - strong RBS - Antifungal GAFP-1 - Double terminator

This expression cassette is designed for the expression and secretion of the Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.

Design

This part is composed of the strong, constitutive promoter of Bacillus subtilis Pveg (K823003), strong RBS (K780002), the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) and a double terminator (B0015). optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.

Type

Generator

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373

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D4E1


BBa_K1364003:RBS - Antifungal D4E1 - Double terminator

D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conodial wall of a varety of fungi.

Design

This part is composed of a Strong RBS (K780002), the open reading frame of D4E1 and a Double terminator (B0015) optimized for its expression and its secretion in Bacillus subtilis. This part was optimized for the expression and its secretion in Bacillus subtilis thanks to the DNA 2.0 software program.

Type

Translational unit

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.
- Can J Microbiol. 1998 Jun;44(6):514-20.

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BBa_K1364009:Pveg - RBS - Antifungal D4E1 - Double Terminator

D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conodial wall of a varety of fungi.

Design

This part is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003), a strong RBS (K780002), the open reading frame of D4E1 and a double terminator (B0015). This part was optimized for the expression and its secretion in Bacillus subtilis thanks to the DNA 2.0 software program.

Type

Translational unit

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.
- Can J Microbiol. 1998 Jun;44(6):514-20.

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EcAMP-1

BBa_K1364010: Pveg - SpoVG - Antifungal EcAMP-1

EcAMP-1 is an antimicrobial peptide of 37 amino acids originated from the specie Echinochloa crus-galli, a type of wild grass. This peptide has a particular structure : it is helical because of two disulfide bonds.
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.
This part was added to the Registry by the iGEM Utah State team in 2013.

Design

This part is composed of the constitutive promoter Pveg and strong RBS for B. subtilis (K733013) and the open reading frame of EcAMP-1.
The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team and thanks to the Life Technologies GeneArt software program.

Type

Generator

Tests

This part is not tested yet.

References

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BBa_K1364011: Pveg - SpoVG - Antifungal EcAMP-1 - Double terminator

EcAMP-1 is an antimicrobial peptide of 37 amino acids originated from the specie Echinochloa crus-galli, a type of wild grass. This peptide has a particular structure : it is helical because of two disulfide bonds.
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.
This part was added to the Registry by the iGEM Utah State team in 2013.

Design

This part is composed of the constitutive promoter Pveg and strong RBS for B. subtilis (K733013), the open reading frame of EcAMP-1 and a double terminator (B0015).
The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team and thanks to the Life Technologies GeneArt software program.

Type

Generator

Tests

This part is not tested yet.

References

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Fungicide operons

BBa_K1364013: Pveg - SpoVG - Antifungal EcAMP-1 - Double terminator

Design

This part is designed for the co-expression of two different peptides with anti-fungal activities : D4E1 and GAFP-1. It is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003) and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).

Type

Generator

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

See BBa_K1364002 and BBa_K1364003.

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Basic tools

BBa_K1364015: Pveg + RFP

Design

Coming soon!

Type

Reporter

Tests

This part was not tested yet

References

Coming soon!

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BBa_K1364016: PlepA + RFP

Design

Coming soon!

Type

Reporter

Tests

This part was not tested yet

References

Coming soon!

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BBa_K1364017: PlepA + RBS SpoVG

Design

Coming soon!

Type

Composite

Tests

This part was not tested yet

References

Coming soon!

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***

-

Design

Coming soon!

Type

Composite

Tests

This part was not tested yet

References

Coming soon!

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BBa_K1364021: Integrative plasmid for Bacillus subtilis

Design

Coming soon!

Type

Composite

Tests

This part was not tested yet

References

Coming soon!

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Experimental results
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Achievement

iGEM Toulouse 2014

Retrieved from "http://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts"