"
Team:Toulouse/Result/parts
From 2014.igem.org
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<h3 class="title2" style="margin-top:10px; color:#333;">Summary :</h3> | <h3 class="title2" style="margin-top:10px; color:#333;">Summary :</h3> | ||
<ul class="menuleft"> | <ul class="menuleft"> | ||
| - | <li | + | <li><a href="#select1">BBa_K1364000</a></li> |
| - | <li><a href="#select2"> | + | <li><a href="#select2">BBa_K1364004</a></li> |
| - | <li><a href="#select3"> | + | <li><a href="#select3">BBa_K1364005</a></li> |
| + | <li><a href="#select4">BBa_K1364002</a></li> | ||
| + | <li><a href="#select5">BBa_K1364007</a></li> | ||
| + | <li><a href="#select6">BBa_K1364008</a></li> | ||
| + | <li><a href="#select7">BBa_K1364003</a></li> | ||
| + | <li><a href="#select8">BBa_K1364009</a></li> | ||
| + | <li><a href="#select9">BBa_K1364010</a></li> | ||
| + | <li><a href="#select10">BBa_K1364011</a></li> | ||
| + | <li><a href="#select11">BBa_K1364013</a></li> | ||
| + | <li><a href="#select12">BBa_K1364015</a></li> | ||
| + | <li><a href="#select13">BBa_K1364016</a></li> | ||
| + | <li><a href="#select14">BBa_K1364017</a></li> | ||
| + | <li><a href="#select15">BBa_K1364019</a></li> | ||
| + | <li><a href="#select16">BBa_K1364021</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 171: | Line 184: | ||
| - | <p class="title2 | + | <p class="title2">I. Chemotaxis</p> |
<!--K1364000 : RBS + Chimio + terminator--> | <!--K1364000 : RBS + Chimio + terminator--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select1"> |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</a>:N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i><br></p> | + | - <a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</a>: N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i><br></p> |
<p class="texte">This part is designed to enable a N-acetylglucosamine | <p class="texte">This part is designed to enable a N-acetylglucosamine | ||
based chemotaxis in <i>Bacillus subtilis</i>. | based chemotaxis in <i>Bacillus subtilis</i>. | ||
| Line 203: | Line 216: | ||
<!--K1364004 : Promotor Pveg + Chimio--> | <!--K1364004 : Promotor Pveg + Chimio--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select2"> |
- <a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a>: P<sub>veg</sub> + N-acetylatedglucosamine based chemotaxis for <i>B. subtilis</i> | - <a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a>: P<sub>veg</sub> + N-acetylatedglucosamine based chemotaxis for <i>B. subtilis</i> | ||
<br> | <br> | ||
| Line 228: | Line 241: | ||
<br> | <br> | ||
| - | <p class="title2 | + | <p class="title2">II. Binding</p> |
<!--K1364001 : RBS + Binding + Terminator--> | <!--K1364001 : RBS + Binding + Terminator--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select3"> |
- <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>: P<sub>veg</sub> + Chitin Binding Cell Wall protein</p> | - <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>: P<sub>veg</sub> + Chitin Binding Cell Wall protein</p> | ||
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/> | <p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/> | ||
| Line 255: | Line 268: | ||
| - | <p class="title2 | + | <p class="title2"> III. Fungicides</p> |
<p class="title3">GAFP-1</p> | <p class="title3">GAFP-1</p> | ||
| Line 261: | Line 274: | ||
<!--K1364002 : RBS + GAFP-1--> | <!--K1364002 : RBS + GAFP-1--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select4"> |
- <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</p> | - <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</p> | ||
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin | <p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin | ||
| Line 282: | Line 295: | ||
<!--K1364007 : RBS + GAFP-1 + Terminator--> | <!--K1364007 : RBS + GAFP-1 + Terminator--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select5"> |
- <a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</p> | - <a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</p> | ||
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin | <p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin | ||
| Line 304: | Line 317: | ||
<!--K13640008 : Pveg + RBS + GAFP-1 + Terminator--> | <!--K13640008 : Pveg + RBS + GAFP-1 + Terminator--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select6"> |
- <a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p> | - <a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p> | ||
<p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin | <p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin | ||
| Line 333: | Line 346: | ||
<!--K1364003 : RBS + D4E1 + Terminator--> | <!--K1364003 : RBS + D4E1 + Terminator--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select7"> |
- <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</p> | - <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</p> | ||
<p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids | <p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids | ||
| Line 358: | Line 371: | ||
<!--K1364009 : Pveg + RBS + D4E1 + Terminator--> | <!--K1364009 : Pveg + RBS + D4E1 + Terminator--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select8"> |
- <a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p> | - <a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p> | ||
<p class="texte"> | <p class="texte"> | ||
| Line 385: | Line 398: | ||
<!--K1364010 : Pveg RBS + EcAMP --> | <!--K1364010 : Pveg RBS + EcAMP --> | ||
| - | <p class="title3"> | + | <p class="title3" id="select9"> |
- <a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</p> | - <a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</p> | ||
<p class="texte"> | <p class="texte"> | ||
| Line 414: | Line 427: | ||
<p class="title3"> | <p class="title3"> | ||
- <a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p> | - <a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p> | ||
| - | <p class="texte"> | + | <p class="texte" id="select10"> |
EcAMP-1 is an antimicrobial peptide of 37 amino acids | EcAMP-1 is an antimicrobial peptide of 37 amino acids | ||
originated from the specie <i>Echinochloa crus-galli</i>, | originated from the specie <i>Echinochloa crus-galli</i>, | ||
| Line 442: | Line 455: | ||
<!--K1364013 : Pveg + RBS + GAFP + RBS + D4E1 + Terminator--> | <!--K1364013 : Pveg + RBS + GAFP + RBS + D4E1 + Terminator--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select11"> |
- <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p> | - <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p> | ||
<p class="texte"> | <p class="texte"> | ||
| Line 462: | Line 475: | ||
<!--K1364015--> | <!--K1364015--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select12"> |
- <a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</p> | - <a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</p> | ||
<p class="texte"><!--Compléter--> | <p class="texte"><!--Compléter--> | ||
| Line 478: | Line 491: | ||
<!--K1364016--> | <!--K1364016--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select13"> |
- <a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</p> | - <a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</p> | ||
<p class="texte"><!--Compléter--> | <p class="texte"><!--Compléter--> | ||
| Line 494: | Line 507: | ||
<!--K1364017--> | <!--K1364017--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select14"> |
- <a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</p> | - <a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</p> | ||
<p class="texte"><!--Compléter--> | <p class="texte"><!--Compléter--> | ||
| Line 510: | Line 523: | ||
<!--K1364019--> | <!--K1364019--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select15"> |
- <a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>: P<sub>veg</sub> + RBS + Antifungal EcAMP + Double terminator</p> | - <a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>: P<sub>veg</sub> + RBS + Antifungal EcAMP + Double terminator</p> | ||
<p class="texte"><!--Compléter--> | <p class="texte"><!--Compléter--> | ||
| Line 526: | Line 539: | ||
<!--K1364021--> | <!--K1364021--> | ||
| - | <p class="title3"> | + | <p class="title3" id="select16"> |
- <a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></p> | - <a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></p> | ||
<p class="texte"><!--Compléter--> | <p class="texte"><!--Compléter--> | ||
Revision as of 16:38, 15 October 2014
Parts
What did we send to the Registry?
Results > Parts
Summary :
As the chassis B. subtilis is not as used as E. coli,
it was sometimes hard for us to get or find desired parts.
However, in collaboration with other iGEM teams and with a synthesis compagny, we managed to obtain or make the following parts:
Submitted parts
We have deposed 16 new BioBrick parts to the Registry.
All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.
I. Chemotaxis
- BBa_K1364000: N-acetylglucosamine based chemotaxis for Bacillus subtilis
This part is designed to enable a N-acetylglucosamine based chemotaxis in Bacillus subtilis.
Design
The encoded protein is a chimera of two proteins:
- the methyl accepting Chemotaxis protein (McpA from Bacillus subtilis which is required for taxis towards glucose.
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from Vibrio cholerae (VCD).
The chimeric protein contains the intracellular domains of McpA from Bacillus subtilis to enable the transduction of the signal:
these domains correspond to the amino acids 1-37 and 282-610.
The extracellular domain of VCD is inserted between the intracellular regions of McpA
to sense N-acetylglucosamine and corresponds to the amino acids 31-310.
Type
Translation unit
Tests
This part is not tested yet.
References
Uniprot:
- McpA
- N-acetylglucosamine regulated methyl-accepting chemotaxis protein
- BBa_K1364004: Pveg + N-acetylatedglucosamine based chemotaxis for B. subtilis
This part is designed to enable a N-acetylglucosamine based chemotaxis in Bacillus subtilis.
Design
This expression cassette is designed for the expression of an antifungal peptide, D4E1 and for its secretion in Bacillus subtilis. It is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003), strong RBS for B. subtilis (K780002), the open reading frame of D4E1 and a double terminator (B0015)
Type
Generator
Tests
This part was not tested yet.
References
Uniprot:
- McpA
- N-acetylglucosamine regulated methyl-accepting chemotaxis protein
II. Binding
- BBa_K1364005: Pveg + Chitin Binding Cell Wall protein
This part is designed to enable the binding of Bacillus subtilis to the fungi wall made of chitin.
Design
This part is composed of a Cell Wall Binding (CWB) sequence
and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.
The CWB domain has been extracted from LytC (BBa_K316030) of the iGEM 2010 Imperial College team.
The CBD is composed of the Domain 4 of the GbpA protein of Vibrio cholerae,
a protein reported to mediate bacterial attachment to chitin.
It has been shown that the Domain 4 was essential to bind chitin.
Type
Generator
Tests
This part was tested with chitin beads. (See Binding module)
References
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
III. Fungicides
GAFP-1
- BBa_K1364002: RBS - Antifungal GAFP-1
The Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.
Design
This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.
Type
Translational unit
Tests
This part was tested on the fungi Trichoderma reesei (See Fungicides module)
References
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
- BBa_K1364007: RBS + Antifungal GAFP-1 + Double terminator
The Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.
Design
This part is composed of a Strong RBS (K780002), the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) and a double terminator B0015. optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.
Type
Composite part
Tests
This part was tested on the fungi Trichoderma reesei (See Fungicides module)
References
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
- BBa_K1364008: Pveg - strong RBS - Antifungal GAFP-1 - Double terminator
This expression cassette is designed for the expression and secretion of the Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.
Design
This part is composed of the strong, constitutive promoter of Bacillus subtilis Pveg (K823003), strong RBS (K780002), the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) and a double terminator (B0015). optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.
Type
Generator
Tests
This part was tested on the fungi Trichoderma reesei (See Fungicides module)
References
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373
D4E1
- BBa_K1364003:RBS - Antifungal D4E1 - Double terminator
D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conodial wall of a varety of fungi.
Design
This part is composed of a Strong RBS (K780002), the open reading frame of D4E1 and a Double terminator (B0015) optimized for its expression and its secretion in Bacillus subtilis. This part was optimized for the expression and its secretion in Bacillus subtilis thanks to the DNA 2.0 software program.
Type
Translational unit
Tests
This part was tested on the fungi Trichoderma reesei (See Fungicides module)
References
- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.
- Can J Microbiol. 1998 Jun;44(6):514-20.
- BBa_K1364009:Pveg - RBS - Antifungal D4E1 - Double Terminator
D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conodial wall of a varety of fungi.
Design
This part is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003), a strong RBS (K780002), the open reading frame of D4E1 and a double terminator (B0015). This part was optimized for the expression and its secretion in Bacillus subtilis thanks to the DNA 2.0 software program.
Type
Translational unit
Tests
This part was tested on the fungi Trichoderma reesei (See Fungicides module)
References
- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.
- Can J Microbiol. 1998 Jun;44(6):514-20.
EcAMP-1
- BBa_K1364010: Pveg - SpoVG - Antifungal EcAMP-1
EcAMP-1 is an antimicrobial peptide of 37 amino acids
originated from the specie Echinochloa crus-galli,
a type of wild grass.
This peptide has a particular structure : it is helical because of two disulfide bonds.
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.
This part was added to the Registry by the iGEM Utah State team in 2013.
Design
This part is composed of the constitutive promoter Pveg and
strong RBS for B. subtilis
(K733013) and
the open reading frame of EcAMP-1.
The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team
and thanks to the Life Technologies GeneArt software program.
Type
Generator
Tests
This part is not tested yet.
References
- BBa_K1364011: Pveg - SpoVG - Antifungal EcAMP-1 - Double terminator
EcAMP-1 is an antimicrobial peptide of 37 amino acids
originated from the specie Echinochloa crus-galli,
a type of wild grass.
This peptide has a particular structure : it is helical because of two disulfide bonds.
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.
This part was added to the Registry by the iGEM Utah State team in 2013.
Design
This part is composed of the constitutive promoter Pveg and
strong RBS for B. subtilis
(K733013),
the open reading frame of EcAMP-1 and
a double terminator (B0015).
The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team
and thanks to the Life Technologies GeneArt software program.
Type
Generator
Tests
This part is not tested yet.
References
Fungicide operons
- BBa_K1364013: Pveg - SpoVG - Antifungal EcAMP-1 - Double terminator
Design
This part is designed for the co-expression of two different peptides with anti-fungal activities : D4E1 and GAFP-1. It is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003) and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).
Type
Generator
Tests
This part was tested on the fungi Trichoderma reesei (See Fungicides module)
References
See BBa_K1364002 and BBa_K1364003.Basic tools
- BBa_K1364015: Pveg + RFP
Design
Coming soon!
Type
Reporter
Tests
This part was not tested yet
References
Coming soon!
- BBa_K1364016: PlepA + RFP
Design
Coming soon!
Type
Reporter
Tests
This part was not tested yet
References
Coming soon!
- BBa_K1364017: PlepA + RBS SpoVG
Design
Coming soon!
Type
Composite
Tests
This part was not tested yet
References
Coming soon!
- BBa_K1364019: Pveg + RBS + Antifungal EcAMP + Double terminator
Design
Coming soon!
Type
Composite
Tests
This part was not tested yet
References
Coming soon!
- BBa_K1364021: Integrative plasmid for Bacillus subtilis
Design
Coming soon!
Type
Composite
Tests
This part was not tested yet
References
Coming soon!
Used parts
BioBrick |
Description |
Origine |
Module(s) |
Pveg (K316001) + RBS-SpoVG (K143021) : Constitutive promotor Pveg and strong RBS for B. subtilis |
2014 Kit plate 1, 7H |
Fungicides (EcAMP) |
|
Pveg : strong, constitutive promotor of B. subtilis |
2014 Kit plate 1, 20G |
Fungicides (D4E1 GAFP-1) |
|
PlepA : constitutive promotor of B. subtilis.
|
2014 Kit plate 1, 20E |
Fungicides (D4E1) |
|
SpoVG RBS for B. subtilis
|
2014 Kit plate 1, 15A |
Optional |
|
B. subtilis transformation vector with LacI, targets amyE locus. |
2014 Kit plate 1, 2N |
Fungicides |
|
2014 Kit plate 3, 3F |
Fungicides (GAFP-1, EcAMP-1) |
||
Terminator for B. subtilis |
Sent by the Warsaw iGEM team |
||
Strong RBS for B. subtilis |
Sent by the Warsaw iGEM team |
||
Strong RBS for B. subtilis |
Sent by the Warsaw iGEM team |
||
Strong constitutive promotor for B. subtilis |
Sent by the Warsaw iGEM team |
Fungicides |
|
EcAMP-1 |
Sent by the Utah State iGEM team |
Fungicides (EcAMP-1) |
|
Empty backbone vector pSBBS1C for integration into B. subtilis
|
Sent by the Munich iGEM team |
Fungicides | |
Empty backbone vector pSBBS4S for integration intoB. subtilis
|
Sent by the Munich iGEM team |
Fungicides |
|
Reporter vector pSBBS1C-lacZ for B. subtilis |
Sent by the Munich iGEM team |
Fungicides |
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