Team:Toulouse/Result/parts

From 2014.igem.org

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<!--Table-->
<!--Table-->
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<p class="texte">
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<p class="texte"><center>
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<table border="1" width="100%" height="20%" id="Chassis">
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<table border="1" width="100%" height="20%">
<!--1ere ligne-->
<!--1ere ligne-->
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<tr><p class="texte">
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<tr><td><p class="texte"><b>BioBrick</b></p></td>
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<td><b>BioBrick</b></td>
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<td><p class="texte"><b>Description</b></p></td>
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<td><b>Description</b></td>
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<td><p class="texte"><b>Origine</b></p></td>
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<td><b>Origine</b></td>
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<td><p class="texte"><b>Module(s)</b></p></td></tr>
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<td><b>Module(s)</b></td>
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</p></tr>
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<!--2eme ligne-->
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<tr><p class="texte">
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<tr><td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K733013">K733013</a></p></td>
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<td><a href="http://parts.igem.org/Part:BBa_K733013">K733013</a></td>
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<td><p class="texte">P<sub>veg</sub> (<a href="http://parts.igem.org/Part:BBa_K316001">K316001</a>) + RBS-SpoVG (<a href="http://parts.igem.org/Part:BBa_K143021">K143021</a>) : Constitutive promotor P<sub>veg</sub> and strong RBS for <i>B. subtilis</i></p></td>
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<td>P<sub>veg</sub> (<a href="http://parts.igem.org/Part:BBa_K316001">K316001</a>) + RBS-SpoVG (<a href="http://parts.igem.org/Part:BBa_K143021">K143021</a>) : Constitutive promotor P<sub>veg</sub> and strong RBS for <i>B. subtilis</i></td>
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<td><p class="texte">2014 Kit plate 1, 7H</p></td>
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<td>2014 Kit plate 1, 7H</td>
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<td><p class="texte">Fungicides (EcAMP)</p></td>
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<td>Fungicides (EcAMP)</td></p>
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</tr>
</tr>
<!--3eme ligne-->
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<tr>
<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K823003">K823003</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823003">K823003</a></p></td>
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<td>P<sub>veg</sub> : strong, constitutive promotor of <i>B. subtilis</i></td>
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<td><p class="texte">P<sub>veg</sub> : strong, constitutive promotor of <i>B. subtilis</i></p></td>
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<td>2014 Kit plate 1, 20G</td>
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<td><p class="texte">2014 Kit plate 1, 20G</p></td>
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<td>Fungicides (D4E1 GAFP-1)<br>Chemotaxis<br>Binding</td>
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<td><p class="texte">Fungicides (D4E1 GAFP-1)<br>Chemotaxis<br>Binding</p></td>
</tr>
</tr>
<!--4eme ligne-->
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K823002">K823002</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823002">K823002</a></p></td>
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<td>P<sub>lepA</sub> : constitutive promotor of <i>B. subtilis</i>.
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<td><p class="texte">P<sub>lepA</sub> : constitutive promotor of <i>B. subtilis</i>.
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<br>It is the promotor of the lepA gene of <i>B. subtilis</i></td>
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<br>It is the promotor of the lepA gene of <i>B. subtilis</i></p></td>
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<td>2014 Kit plate 1, 20E</td>
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<td><p class="texte">2014 Kit plate 1, 20E</p></td>
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<td>Fungicides (D4E1)<br>Chemotaxis<br>Binding</td>
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<td><p class="texte">Fungicides (D4E1)<br>Chemotaxis<br>Binding</p></td>
</tr>
</tr>
<!--5eme ligne-->
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K606061">K606061</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K606061">K606061</a></p></td>
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<td>SpoVG RBS for <i>B. subtilis</i>
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<td><p class="texte">SpoVG RBS for <i>B. subtilis</i>
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<br>Strong ribosome binding site from subtilis. Works fine in <i>E. coli</i></td>
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<br>Strong ribosome binding site from subtilis. Works fine in <i>E. coli</i></p></td>
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<td>2014 Kit plate 1, 15A</td>
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<td><p class="texte">2014 Kit plate 1, 15A</p></td>
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<td>Optional</td>
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<td><p class="texte">Optional</p></td>
</tr>
</tr>
<!--6eme ligne-->
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<tr>
<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K316027">K316027</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K316027">K316027</a></p></td>
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<td><i>B. subtilis</i> transformation vector with LacI, targets amyE locus.</td>
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<td><p class="texte"><i>B. subtilis</i> transformation vector with LacI, targets amyE locus.</p></td>
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<td>2014 Kit plate 1, 2N</td>
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<td><p class="texte">2014 Kit plate 1, 2N</p></td>
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<td>Fungicides<br>Chemotaxis<br>Binding</td>
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<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
</tr>
</tr>
<!--7eme ligne-->
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<td><a href="http://parts.igem.org/Part:BBa_B0015">B0015</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_B0015">B0015</a></p></td>
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<td>Double terminator (<a href="http://parts.igem.org/Part:BBa_B0010">BBa_B0010</a> + <a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a>)</td>
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<td><p class="texte">Double terminator (<a href="http://parts.igem.org/Part:BBa_B0010">BBa_B0010</a> + <a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a>)</p></td>
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<td>2014 Kit plate 3, 3F</td>
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<td><p class="texte">2014 Kit plate 3, 3F</p></td>
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<td>Fungicides (GAFP-1, EcAMP-1)</td>
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<td><p class="texte">Fungicides (GAFP-1, EcAMP-1)</p></td>
</tr>
</tr>
<!--8eme ligne-->
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K780000">K780000</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780000">K780000</a></p></td>
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<td>Terminator for <i>B. subtilis</i></td>
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<td><p class="texte">Terminator for <i>B. subtilis</i></p></td>
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<td>Sent by the Warsaw iGEM team</td>
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<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
<td></td>
<td></td>
</tr>
</tr>
<!--9eme ligne-->
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<td><a href="http://parts.igem.org/Part:BBa_K780001">K780001</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780001">K780001</a></p></td>
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<td>Strong RBS for <i>B. subtilis</i></td>
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<td><p class="texte">Strong RBS for <i>B. subtilis</i></p></td>
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<td>Sent by the Warsaw iGEM team</td>
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<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
<td></td>
<td></td>
</tr>
</tr>
<!--10eme ligne-->
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<td><a href="http://parts.igem.org/Part:BBa_K780002">K780002</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780002">K780002</a></p></td>
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<td>Strong RBS for <i>B. subtilis</i></td>
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<td><p class="texte">Strong RBS for <i>B. subtilis</i></p></td>
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<td>Sent by the Warsaw iGEM team</td>
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<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
<td></td>
<td></td>
</tr>
</tr>
<!--11eme ligne-->
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<tr>
<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K780003">K780003</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780003">K780003</a></p></td>
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<td>Strong constitutive promotor for <i>B. subtilis</i></td>
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<td><p class="texte">Strong constitutive promotor for <i>B. subtilis</i></p></td>
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<td>Sent by the Warsaw iGEM team</td>
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<td><p class="texte">Sent by the Warsaw iGEM team</p></td>
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<td>Fungicides<br>Chemotaxis<br>Binding</td>
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<td>Fungicides<br>Chemotaxis<br>Binding</p></td>
</tr>
</tr>
<!--12eme ligne-->
<!--12eme ligne-->
<tr>
<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a></p></td>
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<td>EcAMP-1</td>
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<td><p class="texte">EcAMP-1</p></td>
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<td>Sent by the Utah State iGEM team</td>
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<td><p class="texte">Sent by the Utah State iGEM team</p></td>
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<td>Fungicides (EcAMP-1)</td>
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<td><p class="texte">Fungicides (EcAMP-1)</p></td>
</tr>
</tr>
<!--13eme ligne-->
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<tr>
<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K823023">K823023</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823023">K823023</a></p></td>
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<td>Empty backbone vector pSB<sub>BS</sub>1C for integration into <i>B. subtilis</i>
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<td><p class="texte">Empty backbone vector pSB<sub>BS</sub>1C for integration into <i>B. subtilis</i>
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<br>It integrates in the amyE locus</td>
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<br>It integrates in the amyE locus</p></td>
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<td>Sent by the Munich iGEM team</td>
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<td><p class="texte">Sent by the Munich iGEM team</p></td>
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<td>Fungicides<br>Chemotaxis<br>Binding</td>
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<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td></tr>
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</tr>
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<!--14eme ligne-->
<!--14eme ligne-->
<tr>
<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K823022">K823022</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823022">K823022</a></p></td>
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<td>Empty backbone vector pSB<sub>BS</sub>4S for integration into<i>B. subtilis</i>
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<td><p class="texte">Empty backbone vector pSB<sub>BS</sub>4S for integration into<i>B. subtilis</i>
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<br>It integrates in the thrC locus</td>
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<br>It integrates in the thrC locus</p></td>
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<td>Sent by the Munich iGEM team</td>
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<td><p class="texte">Sent by the Munich iGEM team</p></td>
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<td>Fungicides<br>Chemotaxis<br>Binding</td>
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<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
</tr>
</tr>
<!--15eme ligne-->
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K823021">K823021</a></td>
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<td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823021">K823021</a></p></td>
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<td>Reporter vector pSB<sub>BS</sub>1C-lacZ for <i>B. subtilis</i></td>
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<td><p class="texte">Reporter vector pSB<sub>BS</sub>1C-lacZ for <i>B. subtilis</i></p></td>
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<td>Sent by the Munich iGEM team</td>
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<td><p class="texte">Sent by the Munich iGEM team</p></td>
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<td>Fungicides<br>Chemotaxis<br>Binding</td>
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<td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td></tr>
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</tr>
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</table></center>
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</table>
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</p>
</p>

Revision as of 08:33, 12 October 2014

           
        logo-igem            

Parts

What did we send to the Registry?

Results   >   Parts

As the chassis B. subtilis is not as used as E. coli, it was sometimes hard for us the get or find desired parts.
However, with the collaboration of other iGEM and a synthesis compagny, we managed to obtain or make the following parts:

Submitted parts

We have deposed 16 new BioBrick parts to the Registry. All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.

Chemotaxis

- BBa_K1364000:N-acetylglucosamine based chemotaxis for Bacillus subtilis

This part is designed to enable a N-acetylglucosamine based chemotaxis in Bacillus subtilis.

Design:

The encoded protein is a chimera of two proteins:
- the methyl accepting Chemotaxis protein (McpA from Bacillus subtilis which is required for taxis towards glucose.
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from Vibrio cholerae (VCD).

The chimeric protein contains the intracellular domains of McpA from Bacillus subtilis to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids 31-310.

Type

Translation unit

Tests

This part is not tested yet.

References

Uniprot:
- McpA
- N-acetylglucosamine regulated methyl-accepting chemotaxis protein


- BBa_K1364004: Pveg + N-acetylatedglucosamine based chemotaxis for B. subtilis

This part is designed to enable a N-acetylglucosamine based chemotaxis in Bacillus subtilis.

Design:

This expression cassette is designed for the expression of an antifungal peptide, D4E1 and for its secretion in Bacillus subtilis. It is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003), strong RBS for B. subtilis (K780002), the open reading frame of D4E1 and a double terminator (B0015)

Type

Generator

Tests

This part was not tested yet.

References

Uniprot:
- McpA
- N-acetylglucosamine regulated methyl-accepting chemotaxis protein


Binding

- BBa_K1364005: Pveg + Chitin Binding Cell Wall protein

This part is designed to enable the binding of Bacillus subtilis to the fungi wall made of chitin.

Design:

This part is composed of a Cell Wall Binding (CWB) sequence and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.

The CWB domain has been extracted from LytC (BBa_K316030) of the iGEM 2010 Imperial College team.
The CBD is composed of the Domain 4 of the GbpA protein of Vibrio cholerae, a protein reported to mediate bacterial attachment to chitin. It has been shown that the Domain 4 was essential to bind chitin.

Type

Generator

Tests

This part was tested with chitin beeds. (See Binding module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373


Fungicides

GAFP-1


- BBa_K1364002: RBS - Antifungal GAFP-1

The Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.

Design:

This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.

Type

Translational unit

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373


- BBa_K1364007: RBS + Antifungal GAFP-1 + Double terminator

The Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.

Design:

This part is composed of a Strong RBS (K780002), the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) and a double terminator B0015. optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.

Type

Composite part

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373


- BBa_K1364008: Pveg - strong RBS - Antifungal GAFP-1 - Double terminator

This expression cassette is designed for the expression and secretion of the Gastrodia anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.

Design:

This part is composed of the strong, constitutive promoter of Bacillus subtilis Pveg (K823003, strong RBS (K780002, the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) and a double terminator B0015. optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.

Type

Generator

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373


D4E1


- BBa_K1364003:RBS - Antifungal D4E1 - Double terminator

D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conodial wall of a varety of fungi.

Design:

This part is composed of a Strong RBS (K780002, the open reading frame of D4E1 and a Double terminator (B0015) optimized for its expression and its secretion in Bacillus subtilis. This part was optimized for the expression and its secretion in Bacillus subtilis thanks to the DNA 2.0 software program.

Type

Translational unit

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.
- Can J Microbiol. 1998 Jun;44(6):514-20.


- BBa_K1364009:Pveg - RBS - Antifungal D4E1 - Double Terminator

D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conodial wall of a varety of fungi.

Design:

This part is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003), a strong RBS (K780002), the open reading frame of D4E1 and a double terminator (B0015). This part was optimized for the expression and its secretion in Bacillus subtilis thanks to the DNA 2.0 software program.

Type

Translational unit

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.
- Can J Microbiol. 1998 Jun;44(6):514-20.


EcAMP-1

- BBa_K1364010: Pveg - SpoVG - Antifungal EcAMP-1

EcAMP-1 is an antimicrobial peptide of 37 amino acids originated from the specie Echinochloa crus-galli, a type of wild grass. This peptide has a particular structure : it is helical because of two disulfide bonds.
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.
This part was added to the Registry by the iGEM Utah State team in 2013.

Design:

This part is composed of the constitutive promoter Pveg and strong RBS for B. subtilis (K733013) and the open reading frame of EcAMP-1.
The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team and thanks to the Life Technologies GeneArt software program.

Type

Generator

Tests

This part is not tested yet.

References


- BBa_K1364011: Pveg - SpoVG - Antifungal EcAMP-1 - Double terminator

EcAMP-1 is an antimicrobial peptide of 37 amino acids originated from the specie Echinochloa crus-galli, a type of wild grass. This peptide has a particular structure : it is helical because of two disulfide bonds.
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.
This part was added to the Registry by the iGEM Utah State team in 2013.

Design:

This part is composed of the constitutive promoter Pveg and strong RBS for B. subtilis (K733013), the open reading frame of EcAMP-1 and a double terminator (B0015).
The EcAMP-1 part was codon optimized for E. coli by the iGEM Utah State team and thanks to the Life Technologies GeneArt software program.

Type

Generator

Tests

This part is not tested yet.

References


Fungicide operons

- BBa_K1364013: Pveg - SpoVG - Antifungal EcAMP-1 - Double terminator

Design:

This part is designed for the co-expression of two different peptides with anti-fungal activities : D4E1 and GAFP-1. It is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003) and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).

Type

Generator

Tests

This part was tested on the fungi Trichoderma reesei (See Fungicides module)

References

See BBa_K1364002 and BBa_K1364003.


Basic tools

- BBa_K1364015: Pveg + RFP

Design:

Coming soon!

Type

Reporter

Tests

This part was not tested yet

References

Coming soon!


- BBa_K1364016: PlepA + RFP

Design:

Coming soon!

Type

Reporter

Tests

This part was not tested yet

References

Coming soon!


- BBa_K1364017: PlepA + RBS SpoVG

Design:

Coming soon!

Type

Composite

Tests

This part was not tested yet

References

Coming soon!


- BBa_K1364019: Pveg + RBS + Antifungal EcAMP + Double terminator

Design:

Coming soon!

Type

Composite

Tests

This part was not tested yet

References

Coming soon!


- BBa_K1364021: Integrative plasmid for Bacillus subtilis

Design:

Coming soon!

Type

Composite

Tests

This part was not tested yet

References

Coming soon!


Used parts


BioBrick

Description

Origine

Module(s)

K733013

Pveg (K316001) + RBS-SpoVG (K143021) : Constitutive promotor Pveg and strong RBS for B. subtilis

2014 Kit plate 1, 7H

Fungicides (EcAMP)

K823003

Pveg : strong, constitutive promotor of B. subtilis

2014 Kit plate 1, 20G

Fungicides (D4E1 GAFP-1)
Chemotaxis
Binding

K823002

PlepA : constitutive promotor of B. subtilis.
It is the promotor of the lepA gene of B. subtilis

2014 Kit plate 1, 20E

Fungicides (D4E1)
Chemotaxis
Binding

K606061

SpoVG RBS for B. subtilis
Strong ribosome binding site from subtilis. Works fine in E. coli

2014 Kit plate 1, 15A

Optional

K316027

B. subtilis transformation vector with LacI, targets amyE locus.

2014 Kit plate 1, 2N

Fungicides
Chemotaxis
Binding

B0015

Double terminator (BBa_B0010 + BBa_B0012)

2014 Kit plate 3, 3F

Fungicides (GAFP-1, EcAMP-1)

K780000

Terminator for B. subtilis

Sent by the Warsaw iGEM team

K780001

Strong RBS for B. subtilis

Sent by the Warsaw iGEM team

K780002

Strong RBS for B. subtilis

Sent by the Warsaw iGEM team

K780003

Strong constitutive promotor for B. subtilis

Sent by the Warsaw iGEM team

 Fungicides
Chemotaxis
Binding

K1162001

EcAMP-1

Sent by the Utah State iGEM team

Fungicides (EcAMP-1)

K823023

Empty backbone vector pSBBS1C for integration into B. subtilis
It integrates in the amyE locus

Sent by the Munich iGEM team

Fungicides
Chemotaxis
Binding

K823022

Empty backbone vector pSBBS4S for integration intoB. subtilis
It integrates in the thrC locus

Sent by the Munich iGEM team

Fungicides
Chemotaxis
Binding

K823021

Reporter vector pSBBS1C-lacZ for B. subtilis

Sent by the Munich iGEM team

Fungicides
Chemotaxis
Binding


Experimental results
logo
Achievement

iGEM Toulouse 2014

Retrieved from "http://2014.igem.org/Team:Toulouse/Result/parts"