Team:Toulouse/Result/parts

From 2014.igem.org

(Difference between revisions)
 
(25 intermediate revisions not shown)
Line 8: Line 8:
   <link href='http://fonts.googleapis.com/css?family=Open+Sans:800' rel='stylesheet' type='text/css'>
   <link href='http://fonts.googleapis.com/css?family=Open+Sans:800' rel='stylesheet' type='text/css'>
 +
 +
<script type='text/javascript' src='http://ajax.googleapis.com/ajax/libs/jquery/1.9.0/jquery.min.js'></script>
 +
 +
<script type='text/javascript'> $(function(){
 +
  $(window).scroll(function () {
 +
    if ($(this).scrollTop() > 565) {
 +
        $('#column-left').addClass("fixNavigation");
 +
    } else {
 +
        $('#column-left').removeClass("fixNavigation");
 +
        }
 +
    });
 +
  });
 +
</script>
  <!--/* CSS contenu*/-->
  <!--/* CSS contenu*/-->
Line 41: Line 54:
.banniere-content{
.banniere-content{
-
  background-color: rgba(46,204,113, 0.6);
+
  background-color: rgba(130,196,108, 0.7);
padding:28px 28px 0;
padding:28px 28px 0;
position:absolute;
position:absolute;
Line 60: Line 73:
font-size:16px
font-size:16px
}
}
 +
 +
table {
 +
border-width:1px;
 +
border-style:solid;
 +
border-color:black;
 +
width:100%;
 +
}
 +
 +
td {
 +
text-align:center;
 +
vertical-align:middle;
 +
border-width:1px;
 +
border-style:solid;
 +
border-color:black;
 +
}
 +
 +
#column-left{
 +
float:left;
 +
width: 190px;
 +
float: left;
 +
padding: 15px 10px 15px 15px;
 +
border: 1px solid #ccc;
 +
border-radius: 5px;
 +
background-color: #eee;
 +
}
 +
 +
.fixNavigation{
 +
z-index: 9999;
 +
position: fixed;
 +
top:15px;
 +
width:240px;
 +
}
 +
 +
ul.menuleft {
 +
padding-left:10px;
 +
}
 +
 +
 +
ul.menuleft li {
 +
padding-bottom: 10px;
 +
}
 +
 +
ul.menuleft li a{
 +
color:#5a6060;
 +
font-family:'Open Sans';
 +
font-size:14px;
 +
margin:0 0 0 0;
 +
line-height:24px;
 +
}
 +
 +
li.tree {
 +
  display : list-item;
 +
list-style-image: url(http://2014.igem.org/wiki/images/a/a7/388438arbrefleurs.png);
 +
}
Line 83: Line 150:
   <div id="innercontenthome">
   <div id="innercontenthome">
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
-
 
-
 
        
        
-
 
+
<p class="texte">As the chassis <i>Bacillus subtilis</i> is not as used as <i>Escherichia coli</i>,  
-
<p class="texte">As the chassis <i>B. subtilis</i> is not as used as <i>E. coli</i>,  
+
it was sometimes hard for us to get or find desired parts.<br>
-
it was sometimes hard for us the get or find desired parts.<br>
+
However, in collaboration with other iGEM teams and with a synthesis company, we managed to obtain or make the following parts:<br>
-
However, with the collaboration of other iGEM and a synthesis compagny, we managed to obtain or make the following parts:<br>
+
-
</p>
+
-
 
+
-
<p class="title1">Submitted parts</p>
+
-
 
+
-
<p class="texte">
+
-
We have deposed 16 new BioBrick parts to the Registry.
+
-
All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.<br>
+
-
<!--à vérifier et confirmer-->
+
-
</p>
+
-
 
+
-
 
+
-
<p class="title2">Chemotaxis</p>
+
-
 
+
-
<!--K1364000 : RBS + Chimio + terminator-->
+
-
 
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</a>:N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i><br></p>
+
-
<p class="texte">This part is designed to enable a N-acetylglucosamine
+
-
based chemotaxis in <i>Bacillus subtilis</i>.
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">The encoded protein is a chimera of two proteins:<br>
+
-
- the methyl accepting Chemotaxis protein (McpA from <i>Bacillus subtilis</i> which is required for taxis towards glucose.<br>
+
-
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from <i>Vibrio cholerae</i> (VCD).<br>
+
-
<br>
+
-
The chimeric protein contains the intracellular domains of McpA from <i>Bacillus subtilis</i> to enable the transduction of the signal:
+
-
these domains correspond to the amino acids 1-37 and 282-610.
+
-
The extracellular domain of VCD is inserted between the intracellular regions of McpA
+
-
to sense N-acetylglucosamine and corresponds to the amino acids 31-310.<br></p>
+
-
<img src="http://2014.igem.org/wiki/images/a/a2/BBa_K1364000.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translation unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part is not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Uniprot:<br>
+
-
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
+
-
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br></p>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364004 : Promotor Pveg + Chimio-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a>: P<sub>veg</sub> + N-acetylatedglucosamine based chemotaxis for <i>B. subtilis</i>
+
-
<br>
+
-
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,
+
-
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
+
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
-
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of D4E1
+
-
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)</p>
+
-
<img src="http://2014.igem.org/wiki/images/3/35/BBa_K1364004.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Uniprot:<br>
+
-
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
+
-
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br>
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title2">Binding</p>
+
-
 
+
-
<!--K1364001 : RBS + Binding + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>:  P<sub>veg</sub> + Chitin Binding Cell Wall protein</p>
+
-
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of a Cell Wall Binding (CWB) sequence
+
-
and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.<br>
+
-
<br>
+
-
The CWB domain has been extracted from LytC (<a href="http://parts.igem.org/Part:BBa_K316030">BBa_K316030</a>) of the iGEM 2010 Imperial College team.<br>
+
-
The CBD is composed of the Domain 4 of the GbpA protein of <i>Vibrio cholerae</i>,
+
-
a protein reported to mediate bacterial attachment to chitin.
+
-
It has been shown that the Domain 4 was essential to bind chitin.</p>
+
-
<img src="http://2014.igem.org/wiki/images/f/f5/BBa_K1364005.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested with chitin beeds. (See <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results">Binding module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
 
+
-
<p class="title2">Fungicides</p>
+
-
<p class="title3">GAFP-1</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364002 : RBS + GAFP-1-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</p>
+
-
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
+
-
originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
-
GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) and
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.</p>
+
-
<img src="http://2014.igem.org/wiki/images/5/5f/BBa_K1364002.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translational unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
+
-
<br>
+
-
 
+
-
<!--K1364007 : RBS + GAFP-1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</p>
+
-
<p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin,  is a mannose and chitin binding lectin
+
-
originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
-
GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.</p>
+
-
<img src="http://2014.igem.org/wiki/images/4/42/BBa_K1364007.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite part</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
+
-
<br>
+
-
 
+
-
<!--K13640008 : Pveg + RBS + GAFP-1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p>
+
-
<p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin
+
-
originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
+
-
GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>,
+
-
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.</p>
+
-
<img src="http://2014.igem.org/wiki/images/6/67/BBa_K1364008.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title3">D4E1</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364003 : RBS + D4E1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</p>
+
-
<p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids
+
-
which has shown to have antifungal activities
+
-
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
+
-
the open reading frame of D4E1 and
+
-
a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
-
thanks to the DNA 2.0 software program.</p>
+
-
<img src="http://2014.igem.org/wiki/images/b/bb/BBa_K1364003.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translational unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
+
-
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
+
-
 
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364009 : Pveg + RBS + D4E1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p>
+
-
<p class="texte">
+
-
D4E1 is a linear synthetic peptide of 17 amino acids
+
-
which has shown to have antifungal activities
+
-
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
-
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of D4E1 and
+
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
+
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
-
thanks to the DNA 2.0 software program.</p>
+
-
<img src="http://2014.igem.org/wiki/images/4/47/BBa_K1364009.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Translational unit</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
+
-
- Can J Microbiol. 1998 Jun;44(6):514-20.</p>
+
-
<br>
+
-
 
+
-
<p class="title3">EcAMP-1</p>
+
-
 
+
-
<!--K1364010 : Pveg RBS + EcAMP -->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</p>
+
-
<p class="texte">
+
-
EcAMP-1 is an antimicrobial peptide of 37 amino acids
+
-
originated from the specie <i>Echinochloa crus-galli</i>,
+
-
a type of wild grass.
+
-
This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
+
-
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
+
-
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>) and
+
-
the open reading frame of EcAMP-1.<br>
+
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
-
and thanks to the Life Technologies GeneArt software program. </p>
+
-
<img src="http://2014.igem.org/wiki/images/5/5f/BBa_K1364010.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte"> Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part is not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte"><!--à compléter : demander à emeline--></p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364011 : Pveg RBS + EcAMP + Double term-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
+
-
<p class="texte">
+
-
EcAMP-1 is an antimicrobial peptide of 37 amino acids
+
-
originated from the specie <i>Echinochloa crus-galli</i>,
+
-
a type of wild grass.
+
-
This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
+
-
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
+
-
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
+
-
the open reading frame of EcAMP-1 and
+
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
+
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
-
and thanks to the Life Technologies GeneArt software program. </p>
+
-
<img src="http://2014.igem.org/wiki/images/7/74/BBa_K1364011.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part is not tested yet.</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte"><!--à compléter : demander à emeline--></p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title3">Fungicide operons</p>
+
-
+
-
<!--K1364013 : Pveg + RBS + GAFP + RBS + D4E1 + Terminator-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
+
-
<p class="texte">
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">This part is designed for the co-expression of two different peptides
+
-
with anti-fungal activities : D4E1 and GAFP-1.
+
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)
+
-
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).</p>
+
-
<img src="http://2014.igem.org/wiki/images/4/40/BBa_K1364013.png">
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Generator</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
+
-
<p class="title4">References</p>
+
-
See <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a> and <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>.</p>
+
-
<br>
+
-
<p class="title2">Basic tools</p>
+
-
 
+
-
<!--K1364015-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Reporter</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364016-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Reporter</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364017-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364019-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>: P<sub>veg</sub> + RBS + Antifungal EcAMP + Double terminator</p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364021-->
+
-
<p class="title3">
+
-
- <a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></p>
+
-
<p class="texte"><!--Compléter-->
+
-
</p>
+
-
<p class="title4">Design:</p>
+
-
<p class="texte">Coming soon! </p>
+
-
<p class="title4">Type</p>
+
-
<p class="texte">Composite</p>
+
-
<p class="title4">Tests</p>
+
-
<p class="texte">This part was not tested yet</p>
+
-
<p class="title4">References</p>
+
-
<p class="texte">Coming soon!</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--SOUS-TITRE-->
+
-
<p class="title1">Used parts</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--Table-->
+
-
<p class="texte">
+
-
<table border="1" width="100%" height="20%" id="Chassis">
+
-
<!--1ere ligne-->
+
-
<tr><p class="texte">
+
-
<td><b>BioBrick</b></td>
+
-
<td><b>Description</b></td>
+
-
<td><b>Origine</b></td>
+
-
<td><b>Module(s)</b></td>
+
-
</p></tr>
+
-
<!--2eme ligne-->
+
-
<tr><p class="texte">
+
-
<td><a href="http://parts.igem.org/Part:BBa_K733013">K733013</a></td>
+
-
<td>P<sub>veg</sub> (<a href="http://parts.igem.org/Part:BBa_K316001">K316001</a>) + RBS-SpoVG (<a href="http://parts.igem.org/Part:BBa_K143021">K143021</a>) : Constitutive promotor P<sub>veg</sub> and strong RBS for <i>B. subtilis</i></td>
+
-
<td>2014 Kit plate 1, 7H</td>
+
-
<td>Fungicides (EcAMP)</td></p>
+
-
</tr>
+
-
<!--3eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823003">K823003</a></td>
+
-
<td>P<sub>veg</sub> : strong, constitutive promotor of <i>B. subtilis</i></td>
+
-
<td>2014 Kit plate 1, 20G</td>
+
-
<td>Fungicides (D4E1 GAFP-1)<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--4eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823002">K823002</a></td>
+
-
<td>P<sub>lepA</sub> : constitutive promotor of <i>B. subtilis</i>.
+
-
<br>It is the promotor of the lepA gene of <i>B. subtilis</i></td>
+
-
<td>2014 Kit plate 1, 20E</td>
+
-
<td>Fungicides (D4E1)<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--5eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K606061">K606061</a></td>
+
-
<td>SpoVG RBS for <i>B. subtilis</i>
+
-
<br>Strong ribosome binding site from subtilis. Works fine in <i>E. coli</i></td>
+
-
<td>2014 Kit plate 1, 15A</td>
+
-
<td>Optional</td>
+
-
</tr>
+
-
<!--6eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K316027">K316027</a></td>
+
-
<td><i>B. subtilis</i> transformation vector with LacI, targets amyE locus.</td>
+
-
<td>2014 Kit plate 1, 2N</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--7eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_B0015">B0015</a></td>
+
-
<td>Double terminator (<a href="http://parts.igem.org/Part:BBa_B0010">BBa_B0010</a> + <a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a>)</td>
+
-
<td>2014 Kit plate 3, 3F</td>
+
-
<td>Fungicides (GAFP-1, EcAMP-1)</td>
+
-
</tr>
+
-
<!--8eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780000">K780000</a></td>
+
-
<td>Terminator for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td></td>
+
-
</tr>
+
-
<!--9eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780001">K780001</a></td>
+
-
<td>Strong RBS for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td></td>
+
-
</tr>
+
-
<!--10eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780002">K780002</a></td>
+
-
<td>Strong RBS for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td></td>
+
-
</tr>
+
-
<!--11eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780003">K780003</a></td>
+
-
<td>Strong constitutive promotor for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--12eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a></td>
+
-
<td>EcAMP-1</td>
+
-
<td>Sent by the Utah State iGEM team</td>
+
-
<td>Fungicides (EcAMP-1)</td>
+
-
</tr>
+
-
<!--13eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823023">K823023</a></td>
+
-
<td>Empty backbone vector pSB<sub>BS</sub>1C for integration into <i>B. subtilis</i>
+
-
<br>It integrates in the amyE locus</td>
+
-
<td>Sent by the Munich iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--14eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823022">K823022</a></td>
+
-
<td>Empty backbone vector pSB<sub>BS</sub>4S for integration into<i>B. subtilis</i>
+
-
<br>It integrates in the thrC locus</td>
+
-
<td>Sent by the Munich iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--15eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823021">K823021</a></td>
+
-
<td>Reporter vector pSB<sub>BS</sub>1C-lacZ for <i>B. subtilis</i></td>
+
-
<td>Sent by the Munich iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
 
+
-
</table>
+
</p>
</p>
 +
<ul>
 +
<li class="tree"><p class="title1"><a href="http://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts"><font color="green">Submitted parts</p></a></li>
 +
<li class="tree"><p class="title1"><a href="http://2014.igem.org/Team:Toulouse/Result/parts/Used_parts"><font color="green">Used parts</p></a></li>
 +
</ul>
-
<br>
+
</div>
 +
<div class="clear"></div>
      
      
   <!-- Navigation section -->   
   <!-- Navigation section -->   
Line 599: Line 168:
   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
-
     <a href="http://2014.igem.org/Team:Toulouse/Result/experimental-results" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Experimental results
+
     <a href="http://2014.igem.org/Team:Toulouse/Modelling" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Modeling
       <img src="http://2014.igem.org/wiki/images/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
       <img src="http://2014.igem.org/wiki/images/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
     </a>  
     </a>  
Line 608: Line 177:
     <a href="http://2014.igem.org/Team:Toulouse/Result/achievement" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
     <a href="http://2014.igem.org/Team:Toulouse/Result/achievement" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
-
color:#666; font-size:18px;">Achievement</br>
+
color:#666; font-size:18px;">Achievements</br>
       <img src="http://2014.igem.org/wiki/images/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
       <img src="http://2014.igem.org/wiki/images/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
     </a>
     </a>
Line 617: Line 186:
     </div>
     </div>
-
  </div>
 
</body>
</body>
</html>
</html>

Latest revision as of 03:55, 18 October 2014