Team:Toulouse/Result/parts

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     <div class="banniere-content">
     <div class="banniere-content">
       <h2>Parts</h2>
       <h2>Parts</h2>
-
       <p>SubtiTree, a bacterium to save our trees</p>
+
       <p>What did we send to the Registry?</p>
     </div>
     </div>
   </div>
   </div>
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   <div class="fils-ariane" style="width:100%; height:60px; background:#ededed;">
   <div class="fils-ariane" style="width:100%; height:60px; background:#ededed;">
-
   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Results&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;parts</p>  
+
   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Results&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Parts</p>  
   </div>
   </div>
    
    
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   <div id="innercontenthome">
   <div id="innercontenthome">
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
-
 
-
 
        
        
-
<p class="title1">Parts</p>
+
<p class="texte">As the chassis <i>Bacillus subtilis</i> is not as used as <i>Escherichia coli</i>,  
-
 
+
it was sometimes hard for us to get or find desired parts.<br>
-
<p class="texte">As the chassis <i>B. subtilis</i> is not as used as <i>E. coli</i>,  
+
However, in collaboration with other iGEM teams and with a synthesis company, we managed to obtain or make the following parts:<br>
-
it was sometimes hard for us the get or find desired parts.<br>
+
-
However, with the collaboration of other iGEM and a synthesis compagny, we managed to obtain or make the following parts:<br>
+
-
</p>
+
-
 
+
-
<p class="title2">1. Submitted parts</p>
+
-
 
+
-
<p class="texte">
+
-
We have deposed 16 new BioBrick parts to the Registry.
+
-
All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.<br>
+
-
<!--à vérifier et confirmer-->
+
-
</p>
+
-
 
+
-
 
+
-
<p class="title3">Chemotaxis</p>
+
-
 
+
-
<!--K1364000 : RBS + Chimio + terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364000:</b> Strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K780001">K780001</a>)
+
-
+ Chemotaxis code
+
-
+ Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)<br><br>
+
-
<b>N-acetylglucosamine based chemotaxis:</b><br>
+
-
This part is designed to enable a N-acetylglucosamine
+
-
based chemotaxis in <i>Bacillus subtilis</i>.<br><br>
+
-
<b>Design:</b><br>
+
-
The encoded protein is a chimera of two proteins:<br>
+
-
- the methyl accepting Chemotaxis protein (McpA from <i>Bacillus subtilis</i> which is required for taxis towards glucose.<br>
+
-
- the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from <i>Vibrio cholerae</i> (VCD).<br>
+
-
<br><br>
+
-
The chimeric protein contains the intracellular domains of McpA from <i>Bacillus subtilis</i> to enable the transduction of the signal:
+
-
these domains correspond to the amino acids 1-37 and 282-610.
+
-
The extracellular domain of VCD is inserted between the intracellular regions of McpA
+
-
to sense N-acetylglucosamine and corresponds to the amino acids 31-310.<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Translation unit<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
This part was tested with the protocol Plug and pond.
+
-
Link ?<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
Uniprot:<br>
+
-
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
+
-
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364004 : Promotor Pveg + Chimio-->
+
-
<p class="texte">
+
-
<b>BBa_K1364004:</b> Strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>) + RBS, Chemotaxis code and Double terminator (K1364000)<br>
+
-
<br>
+
-
<b>N-acetylglucosamine based chemotaxis:</b><br>
+
-
This part is designed to enable a N-acetylglucosamine
+
-
based chemotaxis in <i>Bacillus subtilis</i>.<br><br>
+
-
<b>Design:</b><br>
+
-
This expression cassette is designed for the expression of an antifungal peptide,
+
-
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
+
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
-
strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of D4E1
+
-
and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>) <br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Expression cassette<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
This part was tested with the protocol Plug and pond.
+
-
Link ?<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
Uniprot:<br>
+
-
- <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
+
-
- <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364006 : Pveg, RBS, Chimio, Terminator, Pveg, RBS, Binding and Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364006:</b>
+
-
P<sub>veg</sub>, RBS, Chemotaxis code, Double terminator(BBa_K1364004)
+
-
+ P<sub>veg</sub>, RBS, Binding, Double terminator(BBa_K1364005).<br>
+
-
<b>Type</b><br>
+
-
Expression cassette<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
This part was not tested.
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
See BBa_K1364004 and BBa_1364005.
+
-
<!--à compléter + lien-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title3">Binding</p>
+
-
 
+
-
<!--K1364001 : RBS + Binding + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364001:</b> Consensus RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K090505">K090505</a>) + Binding code + Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)<br><br>
+
-
<b>Binding module:</b><br>
+
-
This part is designed to enable the binding of <i>Bacillus subtilis</i>
+
-
to the fungi wall made of chitin.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of a Cell Wall Binding (CWB) sequence
+
-
and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.<br>
+
-
<br>
+
-
The CWB domain has been extracted from LytC (<a href="http://parts.igem.org/Part:BBa_K316030">BBa_K316030</a>) of the iGEM 2010 Imperial College team.<br>
+
-
The CBD is composed of the Domain 4 of the GbpA protein of <i>Vibrio cholerae</i>,
+
-
a protein reported to mediate bacterial attachment to chitin.
+
-
It has been shown that the Domain 4 was essential to bind chitin.<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Translation unit<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
This part was tested with chitin beeds.
+
-
Link ?
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
 
+
-
<p class="title3">Fungicides</p>
+
-
<p class="title4">GAFP-1</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364002 : RBS + GAFP-1-->
+
-
<p class="texte">
+
-
<b>BBa_K1364002:</b> Strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) + GAFP-1<br>
+
-
<br>
+
-
<b>GAFP-1: Gastrodia anti-fungal protein</b><br>
+
-
The <i>Gastrodia</i> anti-fungal protein (GAFP-1) is a mannose-binding lectin
+
-
which is able to inhibit the growth of multiples species of plant pathogenic fungi.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a> and
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Composite part<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
Link ?<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
</p>
+
-
 
+
-
<!--K1364007 : RBS + GAFP-1 + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364007:</b> Strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) + GAFP-1 + Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a><br>
+
-
<br>
+
-
<b>GAFP-1: Gastrodia anti-fungal protein</b><br>
+
-
The <i>Gastrodia</i> anti-fungal protein (GAFP-1) is a mannose-binding lectin
+
-
which is able to inhibit the growth of multiples species of plant pathogenic fungi.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Composite part<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
Link ?<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K13640008 : Pveg + RBS + GAFP-1 + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364008:</b>
+
-
Strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a> +
+
-
Strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) +
+
-
GAFP-1 +
+
-
Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a><br>
+
-
<br>
+
-
<b>GAFP-1: Gastrodia anti-fungal protein</b><br>
+
-
The <i>Gastrodia</i> anti-fungal protein (GAFP-1) is a mannose-binding lectin
+
-
which is able to inhibit the growth of multiples species of plant pathogenic fungi.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>,
+
-
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
+
-
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
+
-
and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
The codon optimization was made thanks to the DNA 2.0 software program.<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Expression cassette<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
Link ?<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
+
-
- The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title4">D4E1</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364003 : RBS + D4E1 + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364003:</b> Strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) + D4E1
+
-
+ Double terminator (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0015">B0015</a><br>
+
-
<br>
+
-
<b>D4E1</b><br>
+
-
D4E1 is a linear synthetic peptide of 17 amino acids
+
-
which has shown to have antifungal activities
+
-
by complexing with a sterol present in conodial wall of a varety of fungi.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
+
-
the open reading frame of D4E1 and
+
-
a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)
+
-
optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
+
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
-
thanks to the DNA 2.0 software program.<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Translation unit<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
Link ?<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
+
-
- Can J Microbiol. 1998 Jun;44(6):514-20.<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364009 : Pveg + RBS + D4E1 + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364009:</b> Strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)
+
-
+ RBS, D4E1 code and Double terminator (K1364003)<br>
+
-
<b>D4E1</b><br>
+
-
D4E1 is a linear synthetic peptide of 17 amino acids
+
-
which has shown to have antifungal activities
+
-
by complexing with a sterol present in conodial wall of a varety of fungi.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
+
-
a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
+
-
the open reading frame of D4E1 and
+
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
+
-
This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
+
-
thanks to the DNA 2.0 software program.<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Expression cassette<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
Link ?<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
+
-
- Can J Microbiol. 1998 Jun;44(6):514-20.<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<p class="title4">EcAMP-1</p>
+
-
 
+
-
<!--K1364010 : Pveg RBS + EcAMP -->
+
-
<p class="texte">
+
-
<b>BBa_K1364010:</b> Constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>)
+
-
+ EcAMP-1 (<a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a>)
+
-
<br>
+
-
<b>EcAMP-1</b><br>
+
-
EcAMP-1 is an antimicrobial peptide of 37 amino acids
+
-
originated from the specie <i>Echinochloa crus-galli</i>,
+
-
a type of wild grass.
+
-
This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
+
-
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
+
-
This part was added to the Registry by the iGEM Utah State team in 2013.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of the constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>) and
+
-
the open reading frame of EcAMP-1.<br>
+
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
-
and thanks to the Life Technologies GeneArt software program.
+
-
<br>
+
-
<b>Type</b><br>
+
-
Composite part<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
The electrophoresis and PCR tests were OK.<br>
+
-
<!--à compléter avec les tests--><br>
+
-
<b>References</b><br>
+
-
<br>
+
-
<!--à compléter : demander à emeline-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364011 : Pveg RBS + EcAMP + Double term-->
+
-
<p class="texte">
+
-
<b>BBa_K1364011:</b> Constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>)
+
-
+ EcAMP-1 (<a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a>)
+
-
+ Double terminator (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0015">B0015</a>)
+
-
<br>
+
-
<b>EcAMP-1</b><br>
+
-
EcAMP-1 is an antimicrobial peptide of 37 amino acids
+
-
originated from the specie <i>Echinochloa crus-galli</i>,
+
-
a type of wild grass.
+
-
This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
+
-
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
+
-
This part was added to the Registry by the iGEM Utah State team in 2013.<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is composed of the constitutive promoter P<sub>veg</sub> and
+
-
strong RBS for <i>B. subtilis</i>
+
-
(<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
+
-
the open reading frame of EcAMP-1 and
+
-
a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
+
-
The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
+
-
and thanks to the Life Technologies GeneArt software program.
+
-
<br>
+
-
<b>Type</b><br>
+
-
Expression cassette<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
The test does not work yet.<br>
+
-
<!--à compléter avec les tests-->
+
-
<br>
+
-
<b>References</b><br>
+
-
<br>
+
-
<!--à compléter : demander à emeline-->
+
-
</p>
+
-
 
+
-
 
+
-
<p class="title4">Fungicide operons</p>
+
-
 
+
-
<!--K1364012 : RBS + GAFP + RBS + D4E1 + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364012:</b>
+
-
RBS and GAFP-1 (K1364002) + RBS, D4E1 and double terminator (K1364003)<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is designed for the co-expression of two different peptides
+
-
with anti-fungal activities : D4E1 and GAFP-1.
+
-
It is composed of the translation unit with D4E1 (BBa_K1364002)
+
-
and the composite part with GAFP-1 (BBa_K1364003).<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Translation unit<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
<br>
+
-
<!--à compléter avec les tests-->
+
-
<br>
+
-
<b>References</b><br>
+
-
See BBa_K1364002 and BBa_K1364003.<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
+
-
<!--K1364013 : Pveg + RBS + GAFP + RBS + D4E1 + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364013:</b>
+
-
Strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)
+
-
+ RBS, GAFP-1, RBS, D4E1 and double terminator (K1364012).<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part is designed for the co-expression of two different peptides
+
-
with anti-fungal activities : D4E1 and GAFP-1.
+
-
It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
+
-
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)
+
-
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
Expression cassette<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
<br>
+
-
<!--à compléter avec les tests-->
+
-
<br>
+
-
<b>References</b><br>
+
-
See BBa_K1364002 and BBa_K1364003.<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--K1364014 : Pveg RBS spoVG + EcAMP + RBS +  GAFP + RBS + D4E1 + Terminator-->
+
-
<p class="texte">
+
-
<b>BBa_K1364014:</b>
+
-
Promotor P<sub>veg</sub> RBS spoVG, EcAMP-1 (BBa_K1364010)
+
-
+ RBS, GAFP-1, RBS, D4E1 and double terminator (BBa_K1364012)<br>
+
-
<br>
+
-
<b>Design:</b><br>
+
-
This part contains the three fungicides on the same operon.
+
-
It is composed of the composite part with EcAMP--1 (BBa_K1364010)
+
-
and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).<br>
+
-
<br>
+
-
<b>Type</b><br>
+
-
??<br>
+
-
<br>
+
-
<b>Tests</b><br>
+
-
<br>
+
-
<!--à compléter avec les tests-->
+
-
<br>
+
-
<b>References</b><br>
+
-
See BBa_K1364010, BBa_K1364002 and BBa_K1364003.<br>
+
-
<!--à compléter-->
+
-
</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--SOUS-TITRE-->
+
-
<p class="title2">2. Used parts</p>
+
-
 
+
-
<br>
+
-
 
+
-
<!--Table-->
+
-
<p class="texte">
+
-
<table border="1" width="100%" height="20%" id="Chassis">
+
-
<!--1ere ligne-->
+
-
<tr>
+
-
<td><b>BioBrick</b></td>
+
-
<td><b>Description</b></td>
+
-
<td><b>Origine</b></td>
+
-
<td><b>Module(s)</b></td>
+
-
</tr>
+
-
<!--2eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K733013">K733013</a></td>
+
-
<td>P<sub>veg</sub> (<a href="http://parts.igem.org/Part:BBa_K316001">K316001</a>) + RBS-SpoVG (<a href="http://parts.igem.org/Part:BBa_K143021">K143021</a>) : Constitutive promotor P<sub>veg</sub> and strong RBS for <i>B. subtilis</i></td>
+
-
<td>2014 Kit plate 1, 7H</td>
+
-
<td>Fungicides (EcAMP)</td>
+
-
</tr>
+
-
<!--3eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823003">K823003</a></td>
+
-
<td>P<sub>veg</sub> : strong, constitutive promotor of <i>B. subtilis</i></td>
+
-
<td>2014 Kit plate 1, 20G</td>
+
-
<td>Fungicides (D4E1 GAFP-1)<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--4eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823002">K823002</a></td>
+
-
<td>P<sub>lepA</sub> : constitutive promotor of <i>B. subtilis</i>.
+
-
<br>It is the promotor of the lepA gene of <i>B. subtilis</i></td>
+
-
<td>2014 Kit plate 1, 20E</td>
+
-
<td>Fungicides (D4E1)<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--5eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K606061">K606061</a></td>
+
-
<td>SpoVG RBS for <i>B. subtilis</i>
+
-
<br>Strong ribosome binding site from subtilis. Works fine in <i>E. coli</i></td>
+
-
<td>2014 Kit plate 1, 15A</td>
+
-
<td>Optional</td>
+
-
</tr>
+
-
<!--6eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K316027">K316027</a></td>
+
-
<td><i>B. subtilis</i> transformation vector with LacI, targets amyE locus.</td>
+
-
<td>2014 Kit plate 1, 2N</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--7eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_B0015">B0015</a></td>
+
-
<td>Double terminator (<a href="http://parts.igem.org/Part:BBa_B0010">BBa_B0010</a> + <a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a>)</td>
+
-
<td>2014 Kit plate 3, 3F</td>
+
-
<td>Fungicides (GAFP-1, EcAMP-1)</td>
+
-
</tr>
+
-
<!--8eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780000">K780000</a></td>
+
-
<td>Terminator for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td></td>
+
-
</tr>
+
-
<!--9eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780001">K780001</a></td>
+
-
<td>Strong RBS for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td></td>
+
-
</tr>
+
-
<!--10eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780002">K780002</a></td>
+
-
<td>Strong RBS for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td></td>
+
-
</tr>
+
-
<!--11eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K780003">K780003</a></td>
+
-
<td>Strong constitutive promotor for <i>B. subtilis</i></td>
+
-
<td>Sent by the Warsaw iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--12eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a></td>
+
-
<td>EcAMP-1</td>
+
-
<td>Sent by the Utah State iGEM team</td>
+
-
<td>Fungicides (EcAMP-1)</td>
+
-
</tr>
+
-
<!--13eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823023">K823023</a></td>
+
-
<td>Empty backbone vector pSB<sub>BS</sub>1C for integration into <i>B. subtilis</i>
+
-
<br>It integrates in the amyE locus</td>
+
-
<td>Sent by the Munich iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--14eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823022">K823022</a></td>
+
-
<td>Empty backbone vector pSB<sub>BS</sub>4S for integration into<i>B. subtilis</i>
+
-
<br>It integrates in the thrC locus</td>
+
-
<td>Sent by the Munich iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
<!--15eme ligne-->
+
-
<tr>
+
-
<td><a href="http://parts.igem.org/Part:BBa_K823021">K823021</a></td>
+
-
<td>Reporter vector pSB<sub>BS</sub>1C-lacZ for <i>B. subtilis</i></td>
+
-
<td>Sent by the Munich iGEM team</td>
+
-
<td>Fungicides<br>Chemotaxis<br>Binding</td>
+
-
</tr>
+
-
 
+
-
</table>
+
</p>
</p>
 +
<ul>
 +
<li class="tree"><p class="title1"><a href="https://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts"><font color="green">Submitted parts</p></a></li>
 +
<li class="tree"><p class="title1"><a href="https://2014.igem.org/Team:Toulouse/Result/parts/Used_parts"><font color="green">Used parts</p></a></li>
 +
</ul>
-
<br>
+
</div>
 +
<div class="clear"></div>
      
      
   <!-- Navigation section -->   
   <!-- Navigation section -->   
Line 676: Line 168:
   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
-
     <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Experimental results
+
     <a href="https://2014.igem.org/Team:Toulouse/Modelling" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Modeling
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
     </a>  
     </a>  
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     <a href="https://2014.igem.org/Team:Toulouse/Result/achievement" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
     <a href="https://2014.igem.org/Team:Toulouse/Result/achievement" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
-
color:#666; font-size:18px;">Achievement</br>
+
color:#666; font-size:18px;">Achievements</br>
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
     </a>
     </a>
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     </div>
-
  </div>
 
</body>
</body>
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Latest revision as of 03:55, 18 October 2014

           
        logo-igem            

Parts

What did we send to the Registry?

Results   >   Parts

As the chassis Bacillus subtilis is not as used as Escherichia coli, it was sometimes hard for us to get or find desired parts.
However, in collaboration with other iGEM teams and with a synthesis company, we managed to obtain or make the following parts:

Modeling
logo
Achievements

iGEM Toulouse 2014

Retrieved from "http://2014.igem.org/Team:Toulouse/Result/parts"