Team:Toulouse/Result/parts

From 2014.igem.org

(Difference between revisions)
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<p class="texte">This part is designed to enable a N-acetylglucosamine  
<p class="texte">This part is designed to enable a N-acetylglucosamine  
based chemotaxis in <i>Bacillus subtilis</i>.
based chemotaxis in <i>Bacillus subtilis</i>.
-
<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">The encoded protein is a chimera of two proteins:<br>
<p class="texte">The encoded protein is a chimera of two proteins:<br>
- the methyl accepting Chemotaxis protein (McpA from <i>Bacillus subtilis</i> which is required for taxis towards glucose.<br>
- the methyl accepting Chemotaxis protein (McpA from <i>Bacillus subtilis</i> which is required for taxis towards glucose.<br>
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<br>
<br>
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
<p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
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<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,  
<p class="texte">This expression cassette is designed for the expression of an antifungal peptide,  
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
D4E1 and for its secretion in <i>Bacillus subtilis</i>.
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- <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>:  P<sub>veg</sub> + Chitin Binding Cell Wall protein</p>
- <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>:  P<sub>veg</sub> + Chitin Binding Cell Wall protein</p>
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
<p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
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<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of a Cell Wall Binding (CWB) sequence  
<p class="texte">This part is composed of a Cell Wall Binding (CWB) sequence  
and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.<br>
and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.<br>
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  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
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<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) and
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) and
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
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  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
-
<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)  
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  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
  GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
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<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>,
<p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>,
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
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which has shown to have antifungal activities  
which has shown to have antifungal activities  
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
-
<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
<p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>,
the open reading frame of D4E1 and  
the open reading frame of D4E1 and  
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which has shown to have antifungal activities  
which has shown to have antifungal activities  
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
by complexing with a sterol present in conodial wall of a varety of fungi.</p>
-
<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
<p class="texte">This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>  
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
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EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
-
<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
strong RBS for <i>B. subtilis</i>
strong RBS for <i>B. subtilis</i>
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EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
This part was added to the Registry by the iGEM Utah State team in 2013.</p>
-
<p class="title4">Design:</p>
+
<p class="title4">Design</p>
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
<p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
strong RBS for <i>B. subtilis</i>
strong RBS for <i>B. subtilis</i>
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- <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
- <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
<p class="texte">
<p class="texte">
-
<p class="title4">Design:</p>
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<p class="title4">Design</p>
<p class="texte">This part is designed for the co-expression of two different peptides  
<p class="texte">This part is designed for the co-expression of two different peptides  
with anti-fungal activities : D4E1 and GAFP-1.  
with anti-fungal activities : D4E1 and GAFP-1.  
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="title4">Design:</p>
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<p class="title4">Design</p>
<p class="texte">Coming soon! </p>
<p class="texte">Coming soon! </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="title4">Design:</p>
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<p class="title4">Design</p>
<p class="texte">Coming soon! </p>
<p class="texte">Coming soon! </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="title4">Design:</p>
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<p class="title4">Design</p>
<p class="texte">Coming soon! </p>
<p class="texte">Coming soon! </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="title4">Design:</p>
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<p class="title4">Design</p>
<p class="texte">Coming soon! </p>
<p class="texte">Coming soon! </p>
<p class="title4">Type</p>
<p class="title4">Type</p>
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<p class="texte"><!--Compléter-->
<p class="texte"><!--Compléter-->
</p>
</p>
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<p class="title4">Design:</p>
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<p class="title4">Design</p>
<p class="texte">Coming soon! </p>
<p class="texte">Coming soon! </p>
<p class="title4">Type</p>
<p class="title4">Type</p>

Revision as of 22:03, 13 October 2014