Team:Toulouse/Result/experimental-results

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<p class="title2"> 5. Tips capillary system</p>
<p class="title2"> 5. Tips capillary system</p>
<p class="title3">First tips capillary system</p>
<p class="title3">First tips capillary system</p>
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<p class="texte">This protocol from Imperial College 2011 iGEM team was adapted by our team in several steps (See <a href="http://2014.igem.org/Team:Toulouse/Notebook/Protocols#select8">chemotaxis protocol</a>).
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<p class="texte">This protocol was adapted from The Imperial College 2011 iGEM team (See <a href="http://2014.igem.org/Team:Toulouse/Notebook/Protocols#select8">chemotaxis protocol</a>).
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In the first tips capillary system, we used parafilm to avoid any kind of air disturbance in the tips. The different steps are described below:<br>
In the first tips capillary system, we used parafilm to avoid any kind of air disturbance in the tips. The different steps are described below:<br>
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<p class="texte">For the first experiment we wanted to check if the buffer of the binding assay was compatible with <i>B. subtilis</i> survival. To do that, we tested four bacterial concentrations (from OD 0.1 to OD 0.01). These <i>B. subtilis</i> suspensions were incubated 1 hour at 4°C with 500µL of either Chitin Binding Buffer (CBB) or water. 100 µL cell suspension were plated on LB medium in order to count surviving cells.</br>
<p class="texte">For the first experiment we wanted to check if the buffer of the binding assay was compatible with <i>B. subtilis</i> survival. To do that, we tested four bacterial concentrations (from OD 0.1 to OD 0.01). These <i>B. subtilis</i> suspensions were incubated 1 hour at 4°C with 500µL of either Chitin Binding Buffer (CBB) or water. 100 µL cell suspension were plated on LB medium in order to count surviving cells.</br>
Cells do not seem affected by the presence of CBB or water with estimated ODs of 0.05 or 0.025.
Cells do not seem affected by the presence of CBB or water with estimated ODs of 0.05 or 0.025.
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</br>We observed similar survival rates between cells treated with CBB or water (data not shown).
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</br>We observed similar survival rates between cells treated with CBB or water (these data are not shown on figure 16).
Thus, the experimental conditions of the chitin binding assay are compatible with the bacterial life.</p>
Thus, the experimental conditions of the chitin binding assay are compatible with the bacterial life.</p>
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<center><img src="http://2014.igem.org/wiki/images/e/ea/Graphe_binding_2.png" width="60%"></center>
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<center><img src="http://parts.igem.org/wiki/images/e/e0/Data_not.jpg" width="40%"></center>
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<p class="legend">Figure 16</p>
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<p class="legend">Figure 16: We don't want to bother you with useless data</p>
<p class="title2">2. Binding test using engineered <i>B. subtilis</i></p>
<p class="title2">2. Binding test using engineered <i>B. subtilis</i></p>
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Revision as of 00:29, 18 October 2014