Team:Toulouse/Result/experimental-results

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<p class="texte">What were the results of our experiments ? Click on these next titles to see SubtiTree abilities.</p>
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<p class="texte">How did we validate the three modules and improve our new protocols? Click below to find out…</p>
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<p class="texte"> We performed several tests to demonstrate the chemotaxis ability of the transformed <i>Bacillus subtilis</i> strain towards NAG and we used the wild type bacteria and glucose as a positive glucose.  
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<p class="texte"> We used and developed several protocols to demonstrate the existence of chemotaxis in <i>B. subtilis</i> wild type (WT) strain and SubtiTree bacterium towards N-Acetylglucosamine.  
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<p class="title2">1. Petri Dishes Test </p>
<p class="title2">1. Petri Dishes Test </p>
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<p class="texte"> We first tried to test chemotaxis onto Petri Dishes filled with a 0.3% agar medium. This semi-solid medium allows the bacterial motility. A paper disk containing an attractive compound is placed in the middle of the dish and cells are then loaded in the medium (see Figure 1). This protocol was taken from  the <a href="http://2011.igem.org/Team:Imperial_College_London/Protocols_Chemotaxis">the Imperial College 2011 iGEM team</a>.</p>
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<p class="texte">We first wanted to visualize chemotaxis on Petri dishes. We hoped to obtain pictures with bacteria halos directed or around attractive components and thus tried different protocols. The first protocol was adapted from the one published by <a href="http://2011.igem.org/Team:Imperial_College_London/Protocols_Chemotaxis">the Imperial College 2011 iGEM team</a>. They put the attractive compound on a paper disk in the middle of a Petri dish containing a medium with 0.3% agar. Cells are loaded in this medium (Figure 1).
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<b>We first tried to test chemotaxis onto Petri Dishes filled with a 0.3% agar medium. This semi-solid medium allows the bacterial motility. A paper disk containing an attractive compound is placed in the middle of the dish and cells are then loaded in the medium (see Figure 1). This protocol was taken from  the <a href="http://2011.igem.org/Team:Imperial_College_London/Protocols_Chemotaxis">the Imperial College 2011 iGEM team</a></b>.</p>
<center><img SRC="http://2014.igem.org/wiki/images/0/05/Schema_1.png" alt="schema Figure 1" style="width:500px"></center>
<center><img SRC="http://2014.igem.org/wiki/images/0/05/Schema_1.png" alt="schema Figure 1" style="width:500px"></center>
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<p class="legend">Figure 1: Schema showing how cells are filled in the medium. (A) Pipettes are used to put cells in the medium. (B) Bacteria should move to the attractive compound which diffuses.</p>
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<p class="legend">Figure 1: Scheme showing how cells are filled into the medium. (A) A pipet tip is used to deposit cells in the gelose. (B) Bacteria should move toward the attractive compound which diffuses.</p>
<p class="texte">We did not have any result with WT <i>Bacillus subtilis</i> and glucose as attractive compound (Figure 2-A). <i>B. subtilis</i> is attracted by many other glucides and amino-acids, so we also tried to test diluted glucose in LB medium attractant (Figure 2-B).</p>
<p class="texte">We did not have any result with WT <i>Bacillus subtilis</i> and glucose as attractive compound (Figure 2-A). <i>B. subtilis</i> is attracted by many other glucides and amino-acids, so we also tried to test diluted glucose in LB medium attractant (Figure 2-B).</p>

Revision as of 20:03, 16 October 2014