Team:Toulouse/Result/experimental-results

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<img style="width:450px; " src="http://parts.igem.org/wiki/images/a/a8/Prelim_tests_fung.jpg">
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<p class="legend"> Figure 21: Results of the preliminary tests</p>
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<img style="width:400px; " src="http://parts.igem.org/wiki/images/c/c2/Resultfong.jpg"> <p class="legend">Figure 21</p>
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<img style="width:400px; " src="http://parts.igem.org/wiki/images/c/c2/Resultfong.jpg">
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<img style="width:400px; " src="http://parts.igem.org/wiki/images/9/92/Results_fong_2.jpg"> <p class="legend">Figure 22</p>
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<img style="width:400px; " src="http://parts.igem.org/wiki/images/9/92/Results_fong_2.jpg"> <p class="legend">Figure 22: Results with transformed bacteria.</p>
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<p class="title2">3. <i> In planta </i> tests with SubtiTree<p/>
<p class="title2">3. <i> In planta </i> tests with SubtiTree<p/>
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<img style="width:215px;" img src="http://parts.igem.org/wiki/images/a/af/In_planta.jpg" style="margin-top:5px" />
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<p class="legend"> Figure 23: Injection of SubtiTree in a model plant </p>
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The goal of the project is to introduce the trasnformed bacteria in a diseased tree. So it is necessary to perform <i> in planta </i> tests to judge the fungus-killing abilities of the two strains selected after the previous set of experiments. </br>
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The goal of the project is to introduce the trasnformed bacteria in a diseased tree. So it is necessary to perform <i> in planta </i> tests to judge the fungus-killing abilities of the two strains selected after the previous set of experiments. </br>
SubtiTree is first inoculated in two model plants (<i>Arabidopsis thaliana</i> and <i>Nicotiana benthamiana</i>). After this step, a phytopathogenic fungus (<i>Sclerotinia sclerotiorum</i>) is placed on the leaves.  </br>
SubtiTree is first inoculated in two model plants (<i>Arabidopsis thaliana</i> and <i>Nicotiana benthamiana</i>). After this step, a phytopathogenic fungus (<i>Sclerotinia sclerotiorum</i>) is placed on the leaves.  </br>
These tests were made in association with Sylvain Raffaële and Marielle Barascud of the National Institute for the Agronomic Research laboratory. </br>
These tests were made in association with Sylvain Raffaële and Marielle Barascud of the National Institute for the Agronomic Research laboratory. </br>
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<p class="texte">Twenty-four hours after SubtiTree inoculation, no phenotypic modification of the leaves can be detected. We can conclude that our bacterium, its introduction and the fungicides production in plants don't have deleterious effects.</br>
<p class="texte">Twenty-four hours after SubtiTree inoculation, no phenotypic modification of the leaves can be detected. We can conclude that our bacterium, its introduction and the fungicides production in plants don't have deleterious effects.</br>
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Without proper treatment, the drop of the pyhtopathogenic fungus on <i>Nicotiana benthamiana</i>'s leaves causes a necrosis halo which can be measured after 40h. The lesion size and the number of inoculated sites seem reduced by <i>B. subtilis</i>  expressing DE41 or GAFP1-D4E1, unlike with the WT bacterium. A second set of experiments is expected to be more statistically precise.</br>
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Without proper treatment, the drop of the pyhtopathogenic fungus on <i>Nicotiana benthamiana</i>'s leaves causes a necrosis halo which can be measured after 40h. The lesion size and the number of inoculated sites seem reduced by <i>B. subtilis</i>  expressing DE41 or GAFP1-D4E1, unlike with the WT bacterium. A second set of experiments is expected to be more statistically precise.</br><br></br>
We did not observe any significant results for <i>Arabidopsis thaliana</i> because of the use of two plants batches with different ages.</br>
We did not observe any significant results for <i>Arabidopsis thaliana</i> because of the use of two plants batches with different ages.</br>
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<img style="width:860px;" img src="http://parts.igem.org/wiki/images/f/f1/Results_d4%2B_gafp1.jpg" <p class="legend">Figure 24: Results of <i>in planta</i> test</p>
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<p class="texte">Thanks to the diversity of anti-fungal peptides, this strategy can be adapted to different types of diseases, with different degree of specifity, etc.</p>
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Thanks to the diversity of anti-fungal peptides, this strategy can be adapted to different types of diseases, with different degree of specifity, etc.
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Revision as of 16:45, 15 October 2014