Team:Toulouse/Result/experimental-results

From 2014.igem.org

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<B>Results</B>
<B>Results</B>
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     The first tests were performed with commercial GAFP-1 and D4E1 with different concentrations (12,5µM 25µM 100µM). These tests were performed on different fungal strains sharing the same phylum with Ceratocystis Platani. As Ceratocystis Platani is pathogenic, we couldn't perform tests directly with this fungus. <br>
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After several days at 30°C, the PDA (Potato Dextrose Agar) plates covered with fungus and commercial peptides were analyzed.
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An inhibiton halo was noticeable with commercial D4E1 at 100µM on aspergillus brasiliensis. Less bright halos were also present with lower concentrations. Concerning commercial GAFP-1, no effect was noticeable in the tested conditions. As positive control, a well-known chemical fungicide was used : copper sulfate. The inhibition of the fungal growth was complete at 20mg/ml, and at 10mg/ml a darker halo appeared around the pad filled with copper sulfate.  This corresponds to a sporing halo in response to the stress generated by the fungicide.
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Au vu de ces premiers résultats, nous avons remarqué que de fortes concentrations en antifongiques sont nécessaires pour inhiber la croissance du champignon. Suite à ces tests, de nouvelles conditions ont été adoptées afin de ne pas trop favoriser la croissance du champignon au détriment de la croissance bactérienne. Le milieu de croissance élaboré imite la sève et les incubations ont lieu à température ambiante afin d’être plus proche des conditions trouvées dans l’arbre.
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Revision as of 12:57, 5 October 2014