Team:Toulouse/Result/experimental-results

From 2014.igem.org

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<div class="Sub_title"> 1. Preliminary experiments </div>
<div class="Sub_title"> 1. Preliminary experiments </div>
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<B>Purpose</B>
<B>Purpose</B>
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<br/>The first experiment deals with the culture conditions to see if Bacillus subtilis can resist to a low temperature and with the CBB buffer. To do that, several bacterial concentrations have been tested starting with an OD of 0.1 and diluting this solution to get estimated ODs of 0.05, 0.025, 0.01. These different Bacillus subtilis solutions were incubated 1 hour at 4°C with 500µL of CBB or water. Finally a cell count on Thoma cell was performed.
<br/>The first experiment deals with the culture conditions to see if Bacillus subtilis can resist to a low temperature and with the CBB buffer. To do that, several bacterial concentrations have been tested starting with an OD of 0.1 and diluting this solution to get estimated ODs of 0.05, 0.025, 0.01. These different Bacillus subtilis solutions were incubated 1 hour at 4°C with 500µL of CBB or water. Finally a cell count on Thoma cell was performed.
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<B>Results</B>
<B>Results</B>
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<br/>Thus, the experimental conditions regarding the presence of CBB and the incubation temperature at 4°C do not harm the cell surviving.
<br/>Thus, the experimental conditions regarding the presence of CBB and the incubation temperature at 4°C do not harm the cell surviving.
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FIGURE A METTRE
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<br/>FIGURE A METTRE
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<div class="Sub_title"> 2. Binding test </div>
<div class="Sub_title"> 2. Binding test </div>
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<B>Purpose</B>
<B>Purpose</B>
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<br/>Transformed Bacillus subtilis with the binding module is able to produce a protein composed of the bacterial peptidoglycan bonding of LycT and the GbpA 4th domain of Vibrio cholerae allowing the chitin bonding. The synthetic bacterium is put with special beads composed of the polymer miming the fungal pathogen wall. After several washes, bacteria specificaly attached to the chitin are put on plates and counted.
<br/>Transformed Bacillus subtilis with the binding module is able to produce a protein composed of the bacterial peptidoglycan bonding of LycT and the GbpA 4th domain of Vibrio cholerae allowing the chitin bonding. The synthetic bacterium is put with special beads composed of the polymer miming the fungal pathogen wall. After several washes, bacteria specificaly attached to the chitin are put on plates and counted.
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<B>Results</B>
<B>Results</B>
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<br/>Thus, the binding system seems to function correctly and leads to the bacterial attachment on the chitin.  
<br/>Thus, the binding system seems to function correctly and leads to the bacterial attachment on the chitin.  
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FIGURE A METTRE !
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<br/>FIGURE A METTRE !
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Revision as of 12:22, 2 October 2014