Team:Toulouse/Result/experimental-results

From 2014.igem.org

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We have started new tries using different protocols</p>
We have started new tries using different protocols</p>
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<p class="title2">1. Plug in Pond system
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<p class="title2">2. Plug in Pond system
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Results are not as clear as the first time, but we observe halo around the well with glucose at 250mM with and without tetracycline. We have made tries with N-acetyl-glucosamine and we did not see halo in the tested conditions, thus supposing that our strain <i>B. subtilis</i> 168 is not attracted by N-acetyl-glucosamine.</br>
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Results are not as clear as the first time, but we observe halo around the well with glucose at 250mM with and without tetracycline. We have made tries with N-acetyl-glucosamine and we did not see halo in the tested conditions, thus supposing that our strain <i>B. subtilis</i> 168 is not attracted by N-acetyl-glucosamine. MOI JE VOIS DES HALOS ??? </br>  
However, the results are not enough clear, reliable and reproducible with the plug-in-pond protocol. Another testing protocol was then adopted.  
However, the results are not enough clear, reliable and reproducible with the plug-in-pond protocol. Another testing protocol was then adopted.  
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<p class="title2">2. Capillary test between two tubes also called the tubes test</p>
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<p class="title2">4. Capillary test between two tubes also called the tubes test</p>
<p class="texte">After the experiment of the plug in pond, we decided to construct a system by welding two Eppendorf tubes with a capillary thanks to an electric burner.</p>
<p class="texte">After the experiment of the plug in pond, we decided to construct a system by welding two Eppendorf tubes with a capillary thanks to an electric burner.</p>
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<p class="title2"> 3. Tips capillary system</p>
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<p class="title2"> 5. Tips capillary system</p>
<p class="title3">First tips capillary system</p>
<p class="title3">First tips capillary system</p>
<p class="texte">This protocol comes from Imperial College iGEM team 2011 and was adapted by our team in several steps (See <a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols#select8">chemotaxis protocol</a>).<br>
<p class="texte">This protocol comes from Imperial College iGEM team 2011 and was adapted by our team in several steps (See <a href="https://2014.igem.org/Team:Toulouse/Notebook/Protocols#select8">chemotaxis protocol</a>).<br>

Revision as of 14:24, 16 October 2014