Team:Toulouse/Result/experimental-results

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   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Results&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Experimental results</p>  
   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Results&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Experimental results</p>  
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<p class="texte">Short abstract</p>
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<p style="font-size:1.3em;"><a href="#" onClick="ddaccordion.collapseall('technology'); return false">Collapse all</a>  | <a href="#" onClick="ddaccordion.expandall('technology'); return false">Expand all</a></p>
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<div class="technology"><p class="title1" id="select1">Chemotaxis</p></div>
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<h3 class="title2" style="margin-top:10px; color:#333;">Summary :</h3>
 
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  <li style="margin-top:25px;"><a href="#select1">Chemotaxis</a></li>
 
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  <li><a href="#select2">Binding module</a></li>
 
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  <li><a href="#select3">Fungicides module</a></li>
 
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<p class="title1" id="select1">Chemotaxis
 
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<p class="texte">For this module, we performed several tests to prove the existence of chemotaxis in <i>Bacillus subtilis</i> wild type (WT) strain and SubtiTree bacterium towards N-Acetylglucosamine.
<p class="texte">For this module, we performed several tests to prove the existence of chemotaxis in <i>Bacillus subtilis</i> wild type (WT) strain and SubtiTree bacterium towards N-Acetylglucosamine.
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This protocol on which we worked is taken from a thesis (ref thèse). B.subtilis are grown overnight and if necessary bacteria cells are concentrated by centrifgation. Goal is to obtain a cells density to 8x10⁸ cells/mL. 10mL of bacteria cells are mixed with 15mL of LB medium with 1.% agar maintained at 50°C. We obtain a medium with 0.% agar at final concentration. We add tetracyclin at 25µg/mL thus growth are stopped. Plate are cooled and dryed, then well are made with punch or 1mL tips. In well attractive compound are put (Figure 3). After one hour at room temperature, we take a picture of plates and analysed results.
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This protocol on which we worked is taken from a thesis (ref thèse). B.subtilis are grown overnight and if necessary bacteria cells are concentrated by centrifgation. Goal is to obtain a cells density to 8x10⁸ cells/mL. 10mL of bacteria cells are mixed with 15mL of LB medium with 1.5 % agar maintained at 50°C. We obtain a medium with 0.9 % agar at final concentration. We add tetracyclin at 25µg/mL thus growth are stopped. Plate are cooled and dryed, then well are made with punch or 1mL tips. In well attractive compound are put (Figure 3). After one hour at room temperature, we take a picture of plates and analysed results.
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<b>References:</b></br>
<b>References:</b></br>
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  thesis : Etude de la réponse adaptative à l'oxyde de triméthylamine et de son mécanisme de détection chez Escherichia coli et Shewanella oneidensis, 2008, Claudine Baraquet, université de la méditerranée Aix-Marseille II
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  thesis : Etude de la réponse adaptative à l'oxyde de triméthylamine et de son mécanisme de détection chez Escherichia coli et Shewanella oneidensis, 2008, Claudine Baraquet, université de la méditerranée Aix-Marseille II
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<p class="title2">1. Preliminary experiments</p>
<p class="title2">1. Preliminary experiments</p>
<p class="title3">Purpose</p>
<p class="title3">Purpose</p>
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<p class="texte">Finally, overall results are consistent with the presence of functional binding system.</p>
<p class="texte">Finally, overall results are consistent with the presence of functional binding system.</p>
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<div class="technology"><p class="title1" id="select3">Fungicides module</p></div>
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<p class="title1" id="select3">Fungicides module</p>
 
<p class="title2"> 1. Preliminary experiments</p>
<p class="title2"> 1. Preliminary experiments</p>
<p class="title3">Tests with commercial peptides and controls</p>
<p class="title3">Tests with commercial peptides and controls</p>
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<p class="texte">Thanks to the diversity of anti-fungal peptides, this strategy can be adapted to different types of diseases, with different degree of specifity, etc.</p>
<p class="texte">Thanks to the diversity of anti-fungal peptides, this strategy can be adapted to different types of diseases, with different degree of specifity, etc.</p>
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Revision as of 18:10, 15 October 2014