Team:Toulouse/Result/experimental-results

From 2014.igem.org

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In order to test <i>Bacillus subtilis<i/> mutants, it was essential to find the right balance between the fungal growth and the bacterial one.This condition was necessary to get a high concentration of peptides. In our genetic constructions, these peptides are designed to be exported in the extracellular medium.  
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In order to test <i>Bacillus subtilis</i> mutants, it was essential to find the right balance between the fungal growth and the bacterial one.This condition was necessary to get a high concentration of peptides. In our genetic constructions, these peptides are designed to be exported in the extracellular medium.  
<br><br/>The transformed <i>Bacillus subtilis</i> strains were grown at 37°C during 72h and tested. After centrifugation, the supernatant and the resuspended pellet were placed on pads disposed plates previously seeded with a defined number of conidia (see protocols to have more details). After several days at room temperature, an inhibition halo of <br><i>Trichoderma reesei</i>'s growth was clearly observable for the strain expressing D4E1 gene. The inhibition was even more noticeable with the strain carrying the operon GAFP-1 + D4E1.  
<br><br/>The transformed <i>Bacillus subtilis</i> strains were grown at 37°C during 72h and tested. After centrifugation, the supernatant and the resuspended pellet were placed on pads disposed plates previously seeded with a defined number of conidia (see protocols to have more details). After several days at room temperature, an inhibition halo of <br><i>Trichoderma reesei</i>'s growth was clearly observable for the strain expressing D4E1 gene. The inhibition was even more noticeable with the strain carrying the operon GAFP-1 + D4E1.  
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Inhibition halos are not visible with supernatants, probably because of their low concentrations in the extracellular medium.  
Inhibition halos are not visible with supernatants, probably because of their low concentrations in the extracellular medium.  
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Another effect was noted with the same strains expriming D4E1 and GAFP-1 + D4E1 on another fungus <i>Aspergillus brasiliansis<i/>. This effect is comparable to the one previously noted with low concentration of sulfate copper.  
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Another effect was noted with the same strains expriming D4E1 and GAFP-1 + D4E1 on another fungus <i>Aspergillus brasiliansis</i>. This effect is comparable to the one previously noted with low concentration of sulfate copper.  

Revision as of 09:41, 7 October 2014