Team:Toulouse/Result/achievement

From 2014.igem.org

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       <h2>Achievement</h2>
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       <h2>Achievements</h2>
       <p>Bronze, Silver or Gold Medal? That is the question!</p>
       <p>Bronze, Silver or Gold Medal? That is the question!</p>
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   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Results&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Achievement</p>  
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   <p style="margin:0 auto; color:#696969; width:960px; padding-top:20px; font-size:16px;"> Results&nbsp;&nbsp;&nbsp;>&nbsp;&nbsp;&nbsp;Achievements</p>  
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<h1 class="title1" style="text-align:center;">Let's sum up what we did during this summer!</h1>
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<p class="title1" style="text-align:left;"> 2014 Giant Jamboree Results !</p>
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<p class="texte">  
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After a summer of hard work, our team is proud of the achieved results. The first step of our project was to have a <b>proof of concept</b> of the 3 functions requiered to fight against Canker. Once these results obtained, we decided to move on to next step : In order to get closer to our final objective, the 3 genetic modules have to be assembled and to be tested in <b>model plants</b>. In the short time we had, these tests were only performed with the antifungal module, but the results were very encouraging. So encouraging that we moved on to the third step of our project and think about the <b>real injection of SubtiTree in diseased trees</b>. We then considered the <b>spreading</b> problems and proposed different strategies to avoid this major issue. <b>Modeling</b> enables us to predict the growth and the lifespan of SubtiTree in Plane Trees. <br>
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The 2014 Toulouse iGEM team had a lot of fun during the AMAZING Giant Jamboree in Boston ... And all the work done was rewarded by a GOLD medal and the Best Prize "Innovation in measurement" !  </p>
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Well aware that we are part of the <b>iGEM Community</b>, our team also worked for this community by improving the iGEMer's Bible: the Registry of Standard Biological Parts. We improved the EcAMP BioBrick by adding a promotor, a RBS, but mainly by the addition of a STOP codon. As <i>Bacillus subtilis</i> appears to be for us an  ideal chassis, we also developed new genetic tools to facilitate its utilization. Thanks to numerous interactions with other iGEM teams, we didn't see the time flying by during this <b>amazing summer</b> !
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<p class="title1" style="text-align:left;">Let's sum up what we did during this summer!</p>
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<p class="title2">First step...</p>
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<p class="texte">
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After a summer of hard work, our team is proud of the achieved results. The first step of our project was to have a <b>proof of concept</b> of the three functions requiered to fight against canker. The verifications of our genetic constructions were successful (PCR, Migration on an agarose gel and Sequencing). We could then proceed to specific test of the modules: capillary test, chitin beads test or fungicide tests.</p>
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<p class="title2">Second step...</p>
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<p class="texte">
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Once these results obtained, we decided to move on to the next step: in order to get closer to our final objective, the three genetic modules have to be assembled and to be tested in <b>model plants</b>. In the short time we had, these tests were only performed with the antifungal module, but the results were very encouraging (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">3. In planta tests with SubtiTree</a> in the Fungicides module).
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<p class="title2">Third step...</p>
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<p class="texte">
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So encouraging that we moved on to the third step of our project and think about the <b>real injection of SubtiTree in diseased trees</b>. We then considered the <a href="https://2014.igem.org/Team:Toulouse/Project/Spreading"><b>spreading</b></a> problems and proposed different strategies to avoid this major issue. <a href="https://2014.igem.org/Team:Toulouse/Modelling"><b>Modeling</b></a> enables us to predict the growth and the lifespan of SubtiTree in Plane Trees.  
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<p class="title2">And what comes next?</p>
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<p class="texte"> With  the support of Governmental institutions, scientific organizations and the popular enthusiasm raised by our project, we are still looking for the best way to continue and achieve our goal: save the plane trees and preserve the beauty of the Canal du Midi.<br>
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However, SubtiTree's spreading must be controlled, fungicide production regulated, specifity improved, tests of the whole system performed. In a nutshell, we still have a lot of things to do even if we did our best during the whole summer!</p>
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<p class="title2">iGEM Community</p>
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<p class="texte">
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Well aware that we are part of the <b>iGEM Community</b>, our team also worked for this community by improving the iGEMer's Bible: <a href="http://parts.igem.org/Catalog">the Registry of Standard Biological Parts</a>. <br>
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We improved the EcAMP-1 BioBrick by adding a promotor, a RBS, but mainly by the addition of a STOP codon (<a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>).<br>
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As <i>Bacillus subtilis</i> appears to be for us an  ideal chassis, we also developed new genetic tools to facilitate its utilization. Thanks to numerous interactions with other iGEM teams, we didn't see the time flying by during this <b>amazing summer</b> !</p>
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<center><img style="width:1000px; " src="https://static.igem.org/mediawiki/parts/e/e2/Ach.jpg"></center>
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<p class="title2">It is better to have "well-made rather than a well-filled head"... (Michel de Montaigne)</p>
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<p class="texte">We developed a deep <a href="https://2014.igem.org/Team:Toulouse/ethics">ethical questioning</a>, trying to understand the relationship between Science and the protection of the Beauty. We strongly believe that neglecting the ethical aspect in a scientific project is a mistake. All the team was involved in this part and the reflexions made were very profitable to us.</p>
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<p class="texte">We filled the <a href="https://igem.org/2014_Judging_Form?id=1364">2014 Judging Form</a> thanks to the following accomplishments:</p>
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   <h1 class="title1" style="text-align:center;">Medals Fulfillment</h1>  
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Latest revision as of 19:07, 12 December 2014