Team:Toulouse/Project/binding

From 2014.igem.org

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<ul>
<ul>
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<li class="tree"><p class="texte"><B>Anchor section </B>: the CWB (Cell Wall Binding) domain is extracted from LytC gene and composed the 5' side of our binding module. As previously used by the Imperial College of London 2010 iGEM team, we kept the first 318 bp. We can note the presence of the signal peptide at the beginning of the sequence, from 1 to 24bp.</p></li>
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<li class="tree"><p class="texte"><B>Anchor section </B>: the CWB (Cell Wall Binding) domain is extracted from LytC gene and composed the 5' side of our binding module. As previously used by the 2010 Imperial College of London iGEM team, we kept the first 318 bp. We can note the presence of the signal peptide at the beginning of the sequence, from 1 to 24 bp.</p></li>
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<li class="tree"><p class="texte"><B> Chitin Binding Domain (CBD) section </b>:  the Domain 4 of GbpA from Vibrio Cholerae is able to bind to N-Acetyl Glucosamine oligosacchararides. Also,  the 3' side of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp).</p></li>
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<li class="tree"><p class="texte"><B> Chitin Binding Domain (CBD) section </b>:  the 4th domain of GbpA from <I>Vibrio cholerae </I> is able to bind to N-Acetyl Glucosamine oligosacchararides. Also,  the 3' side of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp).</p></li>
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<li class="tree"><p class="texte"><B>Helical Linker </B>: According to the work of the Imperial College of London 2010 iGEM team, we used the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
+
<li class="tree"><p class="texte"><B>Helical Linker </B>: According to the work of the 2010 Imperial College of London iGEM team, we used the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
</p></li></ul>
</p></li></ul>
<center style="margin:-30px;"><img style="width:500px; " src="http://2014.igem.org/wiki/images/a/a0/Binding.png"></center>
<center style="margin:-30px;"><img style="width:500px; " src="http://2014.igem.org/wiki/images/a/a0/Binding.png"></center>

Revision as of 13:23, 16 October 2014