Team:Toulouse/Project/binding

From 2014.igem.org

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<li class="tree"><p class="texte"><B>Helical Linker </B>: According to the work of the Imperial College of London 2010 iGEM team, we used the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
<li class="tree"><p class="texte"><B>Helical Linker </B>: According to the work of the Imperial College of London 2010 iGEM team, we used the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
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<center><img style="width:500px; " src="http://2014.igem.org/wiki/images/4/40/Construction_binding.png"></center>
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<center style="margin:-30px;"><img style="width:500px; " src="http://2014.igem.org/wiki/images/4/40/Construction_binding.png"></center>
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The sequence has been designed <i>in silico</i> and codon optimized for the transcription in <i>B. subtilis</i>.  
The sequence has been designed <i>in silico</i> and codon optimized for the transcription in <i>B. subtilis</i>.  
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<br>The binding module has been placed under the control of Pveg (<a href="http://parts.igem.org/Part:BBa_K143012"target="_blank">BBa_K143012</a>), a strong constitutive promoter and we used a consensus RBS <a href="http://parts.igem.org/Part:BBa_K090505"target="_blank">BBa_K090505</a> and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015"target="_blank">B0015</a>). The construct has been inserted in an integrative plasmid, pSBBS4S (<a href="http://parts.igem.org/Part:BBa_K823022"target="_blank">BBa_K823022</a>)which comes from the LMU-Munich 2012 iGEM team. </p>  
<br>The binding module has been placed under the control of Pveg (<a href="http://parts.igem.org/Part:BBa_K143012"target="_blank">BBa_K143012</a>), a strong constitutive promoter and we used a consensus RBS <a href="http://parts.igem.org/Part:BBa_K090505"target="_blank">BBa_K090505</a> and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015"target="_blank">B0015</a>). The construct has been inserted in an integrative plasmid, pSBBS4S (<a href="http://parts.igem.org/Part:BBa_K823022"target="_blank">BBa_K823022</a>)which comes from the LMU-Munich 2012 iGEM team. </p>  
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<center style="margin:20px;"><img style="width:500px; " src="http://2014.igem.org/wiki/images/f/f5/BBa_K1364005.png"></center>
<p class="title1">References</p>
<p class="title1">References</p>

Revision as of 12:32, 15 October 2014