Team:Toulouse/Project/binding

From 2014.igem.org

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<p class="title1"> More information about this module </p>
<p class="title1"> More information about this module </p>
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<br>The Binding Module ORF is composed of 3 sections:
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The Binding Module ORF is composed of 3 sections:</p>
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<B> <br>- Anchor section </B>: the CWB (Cell Wall Binding) domain is extracted from LytC gene and composed the 5' side of our binding module. As previously used by the Imperial College of London 2010 iGEM team, we kept the first 318 bp. We can note the presence of the signal peptide at the beginning of the sequence, from 1 to 24bp.  
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<B> <br>- Chitin Binding Domain (CBD) section </b>:  the Domain 4 of GbpA from Vibrio Cholerae is able to bind to N-Acetyl Glucosamine oligosacchararides. Also,  the 3' side of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp).
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<ul>
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<B> <br>- Helical Linker </B>: According to the work of the Imperial College of London 2010 iGEM team, we used the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
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<li class="tree"><p class="texte"><B>Anchor section </B>: the CWB (Cell Wall Binding) domain is extracted from LytC gene and composed the 5' side of our binding module. As previously used by the Imperial College of London 2010 iGEM team, we kept the first 318 bp. We can note the presence of the signal peptide at the beginning of the sequence, from 1 to 24bp.</p></li>
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</p>
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<li class="tree"><p class="texte"><B> Chitin Binding Domain (CBD) section </b>:  the Domain 4 of GbpA from Vibrio Cholerae is able to bind to N-Acetyl Glucosamine oligosacchararides. Also,  the 3' side of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp).</p></li>
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<li class="tree"><p class="texte"><B>Helical Linker </B>: According to the work of the Imperial College of London 2010 iGEM team, we used the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
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</p></li></ul>
<center><img style="width:500px; " src="http://2014.igem.org/wiki/images/4/40/Construction_binding.png"></center>
<center><img style="width:500px; " src="http://2014.igem.org/wiki/images/4/40/Construction_binding.png"></center>
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<p class="title1">References</p>
<p class="title1">References</p>
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<p class="texte">
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<ul>
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- M. Desvaux, E. Dumas, I. Chafsey and M. Hébraud.<b> Protein cell surface display in Gram-positive bacteria: from single protein to macromolecular protein structure </b>. FEMS Microbiol. Lett. 256, 1–15 (2006). <br>
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<li class="tree"><p class="texte">M. Desvaux, E. Dumas, I. Chafsey and M. Hébraud.<b> Protein cell surface display in Gram-positive bacteria: from single protein to macromolecular protein structure </b>. FEMS Microbiol. Lett. 256, 1–15 (2006). </p></li>
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-E. Wong, G. Vaaje-Kolstad, A. Ghosh, R. Hurtado-Guerrero, PV. Konarev, AF. Ibrahim, DI. Svergun, VG. Eijsink, NS. Chatterjee and DM. van Aalten.<b>The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces</b>. PLoS Pathog. 8, e1002373 (2012).<br>
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<li class="tree"><p class="texte">E. Wong, G. Vaaje-Kolstad, A. Ghosh, R. Hurtado-Guerrero, PV. Konarev, AF. Ibrahim, DI. Svergun, VG. Eijsink, NS. Chatterjee and DM. van Aalten.<b>The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces</b>. PLoS Pathog. 8, e1002373 (2012).</p></li>
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-H. Yamamoto, S. Kurosawa and J. Sekiguchi. <b>Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases</b>.  J. Bacteriol. 185, 6666–6677 (2003).<br>
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<li class="tree"><p class="texte">H. Yamamoto, S. Kurosawa and J. Sekiguchi. <b>Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases</b>.  J. Bacteriol. 185, 6666–6677 (2003).</p></li>
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</ul>
      
      

Revision as of 11:31, 15 October 2014