Team:Toulouse/Project/binding

From 2014.igem.org

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<B> <br>- Helical Linker </B>: according to the work of the Imperial College of London 2010 iGEM team, we use the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
<B> <br>- Helical Linker </B>: according to the work of the Imperial College of London 2010 iGEM team, we use the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
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<img style="width:400px; " src="http://2014.igem.org/wiki/images/4/40/Construction_binding.png">
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<center><img style="width:700px; " src="http://2014.igem.org/wiki/images/4/40/Construction_binding.png"></center>
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<B class="title1">Final construction</B>  
<B class="title1">Final construction</B>  
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<p class="texte">
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<br>To introduce the Binding Module in Bacillus subtilis chromosome, we insert the ORF Binding Module in pSBBS4S plasmid (BBa_K823022), from the LMU-Munich 2012 iGEM team, with:
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<br>To introduce the Binding Module in Bacillus subtilis chromosome, we insert the ORF Binding Module in pSBBS4S plasmid (<a href="http://parts.igem.org/Part:BBa_K823022"target="_blank">BBa_K823022</a>), from the LMU-Munich 2012 iGEM team, with:
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<br>- <B> Regulatory section </B>: after the ORF Binding Module, we include Pveg (BBa_K143012) as strong promoter and iGEM RBS consensus (BBa_K090505).
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<br>- <B> Regulatory section </B>: after the ORF Binding Module, we include Pveg (<a href="http://parts.igem.org/Part:BBa_K143012"target="_blank">BBa_K143012</a>) as strong promoter and iGEM RBS consensus (<a href="http://parts.igem.org/Part:BBa_K090505"target="_blank">BBa_K090505</a>).
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<br>- <B> Transcription end </B> : we use a double terminator (BBa_B0015)
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<br>- <B> Transcription end </B> : we use a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015"target="_blank">BBa_B0015</a>)
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<img style="width:400px; " src="http://2014.igem.org/wiki/images/f/f5/BBa_K1364005.png">
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<center><img style="width:700px; " src="http://2014.igem.org/wiki/images/f/f5/BBa_K1364005.png"></center>
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<b class="title1">References</p>
<b class="title1">References</p>

Revision as of 11:14, 13 October 2014