Team:Toulouse/Project/binding

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         <p class="texte"> The second step in the SubtiTree optimization matches with the <B> binding ability </B> of our bacterium. Thus, we design a chimeric protein (BBa_K1364005) able to make <B> a bridge between bacterial peptidoglycan and fungal chitin </B>, the main component of the pathogen’s cell wall. According to the Imperial College of London 2010 iGEM team, we use CWB domain of LytC protein to bind our chimeric protein to <I> Bacillus subtilis </I> cell wall. On the other side of our protein, we add the fragment of  GbpA from <I> Vibrio Cholerae </I>, which is known to recognize N-Acetyl Glucosamine oligosaccharides called chitin.
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         <p class="texte"> The second step in the SubtiTree optimization matches with the <B> binding ability </B> of our bacterium. Thus, we design a chimeric protein (<a href="http://parts.igem.org/Part:BBa_K1364005"target="_blank">BBa_K1364005</a>) able to make <B> a bridge between bacterial peptidoglycan and fungal chitin </B>, the main component of the pathogen’s cell wall. According to the Imperial College of London 2010 iGEM team, we use CWB domain of LytC protein to bind our chimeric protein to <I> Bacillus subtilis </I> cell wall. On the other side of our protein, we add the fragment of  GbpA from <I> Vibrio Cholerae </I>, which is known to recognize N-Acetyl Glucosamine oligosaccharides called chitin.
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Revision as of 11:09, 13 October 2014