Team:Toulouse/Project/binding

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<B> More information about this module </B>
<B> More information about this module </B>
<p> The Binding Module ORF is composed of 3 sections:
<p> The Binding Module ORF is composed of 3 sections:
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<br>- <B> Anchor section </B>: the CWB (Cell Wall Binding) is a LytC domain put on 5' of our chimeric protein gene. As previously used by the Imperial College of London 2010 iGEM team, we retain the first 318 bp. We can note the presence of the signal peptide at the beginning from 1 to 24 bp.  
+
<B> <br>- Anchor section </B>: the CWB (Cell Wall Binding) is a LytC domain put on 5' of our chimeric protein gene. As previously used by the Imperial College of London 2010 iGEM team, we retain the first 318 bp. We can note the presence of the signal peptide at the beginning from 1 to 24 bp.  
-
<br>- <B> Chitin Binding Domain (CBD) section </B:  the Domain 4 of GbpA from Vibrio Cholerae is able to bind to N-Acetyl Glucosamine oligosacchararides. Also, the last base pairs in 3' of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp).
+
<B> <br>- Chitin Binding Domain (CBD) section </B:  the Domain 4 of GbpA from Vibrio Cholerae is able to bind to N-Acetyl Glucosamine oligosacchararides. Also, the last base pairs in 3' of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp).
-
- <br><B> Helical Linker </B>: according to the work of the Imperial College of London 2010 iGEM team, we use the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
+
<B> <br>- Helical Linker </B>: according to the work of the Imperial College of London 2010 iGEM team, we use the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
</p>
</p>

Revision as of 12:46, 1 October 2014