"
Team:Toulouse/Project/binding
From 2014.igem.org
(Created page with "{{:Team:Toulouse/template/header-base}} <html> <style type="text/css"> .banniere{ background: url('https://static.igem.org/mediawiki/2014/d/d0/Template-igem2014-slide1.jpg') no-repe...") |
|||
| Line 62: | Line 62: | ||
<!--Short description : à changer!!!--> | <!--Short description : à changer!!!--> | ||
| - | <p> | + | <p> The second step in the SubtiTree optimization matches with the binding ability of our bacterium. Thus, we design a chimeric protein (BBa_K1364005) able to make a bridge between bacterial peptidoglycan and fungal chitin, the main component of the pathogen’s cell wall. According to the Imperial College of London 2010 iGEM team, we use CWB domain of LytC protein to bind our chimeric protein to Bacillus subtilis cell wall. On the other side of our protein, we add acfragment of GbpA from Vibrio Cholerae, which is known to recognise N-Acetyl Glucosamine oligosaccharides called chitin.</p> |
| - | <p> | + | <B> More information about this module </B> |
| + | <p> The Binding Module ORF is composed of 3 sections: | ||
| + | <br>- Anchor section: the CWB (Cell Wall Binding) is a LytC domain put on 5' of our chimeric protein gene. As previously used by the Imperial College of London 2010 iGEM team, we retain the first 318 bp. We can note the presence of the signal peptide at the beginning from 1 to 24 bp. | ||
| + | <br>- Chitin Binding Domain (CBD) section: the Domain 4 of GbpA from Vibrio Cholerae is able to bind to N-Acetyl Glucosamine oligosacchararides. Also, the last base pairs in 3' of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp). | ||
| + | <br>- Helical Linker: according to the work of the Imperial College of London 2010 iGEM team, we use the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section. | ||
| + | </p> | ||
<p>Ut at massa quam. Vestibulum rutrum dapibus tortor ut facilisis. Pellentesque condimentum quam id ante varius imperdiet. Quisque mauris urna, finibus in mauris vel, laoreet volutpat libero. Aliquam erat volutpat. Vivamus non imperdiet nibh. Sed fringilla tristique metus, non fermentum quam tincidunt non. Praesent dictum faucibus massa, eu bibendum sapien iaculis in. Ut porta tortor eu elementum interdum. Vivamus non tempor ligula. In accumsan ligula non ligula aliquam, at dictum dolor cursus. Ut iaculis ultrices ex et faucibus. Praesent bibendum sagittis elit, eget ultricies dolor imperdiet id.</P> | <p>Ut at massa quam. Vestibulum rutrum dapibus tortor ut facilisis. Pellentesque condimentum quam id ante varius imperdiet. Quisque mauris urna, finibus in mauris vel, laoreet volutpat libero. Aliquam erat volutpat. Vivamus non imperdiet nibh. Sed fringilla tristique metus, non fermentum quam tincidunt non. Praesent dictum faucibus massa, eu bibendum sapien iaculis in. Ut porta tortor eu elementum interdum. Vivamus non tempor ligula. In accumsan ligula non ligula aliquam, at dictum dolor cursus. Ut iaculis ultrices ex et faucibus. Praesent bibendum sagittis elit, eget ultricies dolor imperdiet id.</P> | ||
Revision as of 12:00, 1 October 2014
Binding
Proin sollicitudin nibh ut dapibus vulputate.
Project > Binding
The second step in the SubtiTree optimization matches with the binding ability of our bacterium. Thus, we design a chimeric protein (BBa_K1364005) able to make a bridge between bacterial peptidoglycan and fungal chitin, the main component of the pathogen’s cell wall. According to the Imperial College of London 2010 iGEM team, we use CWB domain of LytC protein to bind our chimeric protein to Bacillus subtilis cell wall. On the other side of our protein, we add acfragment of GbpA from Vibrio Cholerae, which is known to recognise N-Acetyl Glucosamine oligosaccharides called chitin.
More information about this module The Binding Module ORF is composed of 3 sections:
- Anchor section: the CWB (Cell Wall Binding) is a LytC domain put on 5' of our chimeric protein gene. As previously used by the Imperial College of London 2010 iGEM team, we retain the first 318 bp. We can note the presence of the signal peptide at the beginning from 1 to 24 bp.
- Chitin Binding Domain (CBD) section: the Domain 4 of GbpA from Vibrio Cholerae is able to bind to N-Acetyl Glucosamine oligosacchararides. Also, the last base pairs in 3' of our gene is composed by a part of the GbpA sequence (from 423 to 484 bp).
- Helical Linker: according to the work of the Imperial College of London 2010 iGEM team, we use the same six amino acids sequence (SRGSRA) to make a bridge between the Anchor section and the Chitin Binding section.
Ut at massa quam. Vestibulum rutrum dapibus tortor ut facilisis. Pellentesque condimentum quam id ante varius imperdiet. Quisque mauris urna, finibus in mauris vel, laoreet volutpat libero. Aliquam erat volutpat. Vivamus non imperdiet nibh. Sed fringilla tristique metus, non fermentum quam tincidunt non. Praesent dictum faucibus massa, eu bibendum sapien iaculis in. Ut porta tortor eu elementum interdum. Vivamus non tempor ligula. In accumsan ligula non ligula aliquam, at dictum dolor cursus. Ut iaculis ultrices ex et faucibus. Praesent bibendum sagittis elit, eget ultricies dolor imperdiet id.
Socialize
