Team:Toulouse/Project/binding

From 2014.igem.org

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<B class="title1"> More information about this module </B>
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<p class="title1"> More information about this module </p>
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<br>The Binding Module ORF is composed of 3 sections:
<br>The Binding Module ORF is composed of 3 sections:
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<br>To introduce the Binding Module in Bacillus subtilis chromosome, we insert the ORF Binding Module in pSBBS4S plasmid (<a href="http://parts.igem.org/Part:BBa_K823022"target="_blank">BBa_K823022</a>), from the LMU-Munich 2012 iGEM team, with:
<br>To introduce the Binding Module in Bacillus subtilis chromosome, we insert the ORF Binding Module in pSBBS4S plasmid (<a href="http://parts.igem.org/Part:BBa_K823022"target="_blank">BBa_K823022</a>), from the LMU-Munich 2012 iGEM team, with:
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<br>- <B> Regulatory section </B>: after the ORF Binding Module, we include Pveg (<a href="http://parts.igem.org/Part:BBa_K143012"target="_blank">BBa_K143012</a>) as strong promoter and iGEM RBS consensus (<a href="http://parts.igem.org/Part:BBa_K090505"target="_blank">BBa_K090505</a>).
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<br>- <B> Regulatory section</B>: after the ORF Binding Module, we include Pveg (<a href="http://parts.igem.org/Part:BBa_K143012"target="_blank">BBa_K143012</a>) as strong promoter and iGEM RBS consensus (<a href="http://parts.igem.org/Part:BBa_K090505"target="_blank">BBa_K090505</a>).
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<br>- <B> Transcription end </B> : we use a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015"target="_blank">BBa_B0015</a>)
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<br>- <B> Transcription end</B>: we use a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015"target="_blank">BBa_B0015</a>)
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<center><img style="width:500px; " src="https://static.igem.org/mediawiki/2014/f/f5/BBa_K1364005.png"></center>
<center><img style="width:500px; " src="https://static.igem.org/mediawiki/2014/f/f5/BBa_K1364005.png"></center>
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<b class="title1">References</p>
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<p class="title1">References</p>
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Revision as of 18:31, 13 October 2014