Team:Toulouse/Project/binding

From 2014.igem.org

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a <B>bridge between the bacterial peptidoglycan and the fungal chitin</B>, the main component of the pathogen’s cell wall.  
a <B>bridge between the bacterial peptidoglycan and the fungal chitin</B>, the main component of the pathogen’s cell wall.  
According to the work of <a href="https://2010.igem.org/Team:Imperial_College_London"target="_blank">the Imperial College 2010</a> iGEM team,  
According to the work of <a href="https://2010.igem.org/Team:Imperial_College_London"target="_blank">the Imperial College 2010</a> iGEM team,  
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we used the Cell Wall Binding (CWB) domain of the LytC protein (coding for a N-acetylmuramoyl-L-alanine amidase) to attach our chimeric protein  
+
we used the Cell Wall Binding (CWB) domain of the <a href="http://www.uniprot.org/uniprot/Q02114"_blanck">LytC</a> protein (coding for a N-acetylmuramoyl-L-alanine amidase) to attach our chimeric protein  
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to the <I>B. subtilis</I> cell wall. On the other side of our protein, we added the domain 4 of GbpA from <I>Vibrio cholerae</I>, which is known to  
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to the <I>B. subtilis</I> cell wall. On the other side of our protein, we added the domain 4 of <a href="http://www.uniprot.org/uniprot/Q9KLD5"_blanck">GbpA</a> from <I>Vibrio cholerae</I>, which is known to  
recognize chitin.
recognize chitin.
</p>
</p>

Revision as of 00:34, 18 October 2014