(Difference between revisions)
 Revision as of 09:41, 7 October 2014 (view source)Kanitzer (Talk | contribs)← Older edit Revision as of 09:42, 7 October 2014 (view source)Kanitzer (Talk | contribs) Newer edit → Line 97: Line 97:
Auxotrophy
Auxotrophy
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Non-sporing
Non-sporing
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Integrative plasmid ?
Integrative plasmid ?
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All our constructions should be carried by integrative plasmids (pS.., pS.., pS..). Consequently, our different genetic modules would be integrated in the bacterium genome. The integration in the genome is more stable as the constructions are less likely to be transferred to other microorganisms. In addition to that, the expression of our genetic modules would not be dependant on a selective pressure based on an antibiotic resistance (as we can not inject antibiotics in the tree), allowing a high level of transcription in planta. All our constructions should be carried by integrative plasmids (pS.., pS.., pS..). Consequently, our different genetic modules would be integrated in the bacterium genome. The integration in the genome is more stable as the constructions are less likely to be transferred to other microorganisms. In addition to that, the expression of our genetic modules would not be dependant on a selective pressure based on an antibiotic resistance (as we can not inject antibiotics in the tree), allowing a high level of transcription in planta.