Team:Toulouse/Project/Spreading

From 2014.igem.org

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       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
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<p>How can SubtiTree respect the environment ? How do we keep control on Subtitree?</p>
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<p class="title2">How can SubtiTree respect the environment ? How do we keep control on Subtitree?</p>
<p class="texte">Our engineered bacterium has been designed to be inoculated in a tree and to cure fungal diseases. Understanding the environmental issues resulting from the use of a modified organism in the nature, our team worked on different aspects in order to ensure a safe use of SubtiTree.  
<p class="texte">Our engineered bacterium has been designed to be inoculated in a tree and to cure fungal diseases. Understanding the environmental issues resulting from the use of a modified organism in the nature, our team worked on different aspects in order to ensure a safe use of SubtiTree.  
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<div class="Sub_title"><div id="Auxotrophy">Auxotrophy</div></div>
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<div class="Sub_title title2simple"><div id="Auxotrophy">Auxotrophy</div></div>
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To make the bacterium dependant on the tree and to avoid its spreading in the environment, it should be preferable to use a strain of B.subtilis which is auxotroph to a particular amino acid. The bacterium should be unable to synthesize one essential amino acid, and should find it in its environment. The glutamine could be a good example since it is wide-spread in the phloem sap. It is the amino acid which is present in highest concentration in the phloem sap. If our bacterium is unable to synthesize the glutamine, it will be obliged to take it in its close environment, that is to say the phloem sap.  
To make the bacterium dependant on the tree and to avoid its spreading in the environment, it should be preferable to use a strain of B.subtilis which is auxotroph to a particular amino acid. The bacterium should be unable to synthesize one essential amino acid, and should find it in its environment. The glutamine could be a good example since it is wide-spread in the phloem sap. It is the amino acid which is present in highest concentration in the phloem sap. If our bacterium is unable to synthesize the glutamine, it will be obliged to take it in its close environment, that is to say the phloem sap.  
Thus, if the bacterium is in the sap, it can grow normally without any deficiency since it uses the glutamine present in the sap ; but if it escapes from the tree and a fortiori from the sap, it will not be able to survive for a long time. Indeed, glutamine is found in very low quantities in the ground. This system should guarantee that the bacterium develops only in the tree and not elsewhere in the surroundings of the tree.<br/>  
Thus, if the bacterium is in the sap, it can grow normally without any deficiency since it uses the glutamine present in the sap ; but if it escapes from the tree and a fortiori from the sap, it will not be able to survive for a long time. Indeed, glutamine is found in very low quantities in the ground. This system should guarantee that the bacterium develops only in the tree and not elsewhere in the surroundings of the tree.<br/>  
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<div class="Sub_title"><div id="NonSporing">Non-sporing </div></div>
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<div class="Sub_title title2simple"><div id="NonSporing">Non-sporing </div></div>
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<div class="Sub_title"><div id="Toxin">Toxin-antitoxin system</div></div>
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<div class="Sub_title title2simple"><div id="Toxin">Toxin-antitoxin system</div></div>
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<div class="Sub_title title2">Integrative plasmid ?</div>
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<div class="Sub_title title2simple">Integrative plasmid ?</div>
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Revision as of 09:41, 7 October 2014