Team:Toulouse/Project/Spreading

From 2014.igem.org

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To make the bacterium dependant on the tree and to avoid its spreading in the environment, it should be preferable to use a strain of <i>B.subtilis</i> which is auxotroph to a particular amino acid. The bacterium should be unable to synthesize one essential amino acid, and should find it in its environment. The glutamine could be a good example since it is wide-spread in the phloem sap. It is the amino acid which is present in highest concentration in the phloem sap. If our bacterium is unable to synthesize the glutamine, it will be obliged to take it in its close environment, that is to say the phloem sap.  
To make the bacterium dependant on the tree and to avoid its spreading in the environment, it should be preferable to use a strain of <i>B.subtilis</i> which is auxotroph to a particular amino acid. The bacterium should be unable to synthesize one essential amino acid, and should find it in its environment. The glutamine could be a good example since it is wide-spread in the phloem sap. It is the amino acid which is present in highest concentration in the phloem sap. If our bacterium is unable to synthesize the glutamine, it will be obliged to take it in its close environment, that is to say the phloem sap.  
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Thus, if the bacterium is in the sap, it can grow normally without any deficiency since it uses the glutamine present in the sap ; but if it escapes from the tree and a fortiori from the sap, it will not be able to survive for a long time. Indeed, glutamine is found in very low quantities in the ground. This system should guarantee that the bacterium develops only in the tree and not elsewhere in the surroundings of the tree.<br/>  
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Thus, if the bacterium is in the sap, it can grow normally without any deficiency since it uses the glutamine present in the sap ; but if it escapes from the tree and <i>a fortiori</i> from the sap, it will not be able to survive for a long time. Indeed, glutamine is found in low quantities in the ground. This system should guarantee that the bacterium develops only in the tree and not elsewhere in the surroundings of the tree.<br/>  
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Auxotroph <i>B.sutbilis</i> strains already exist and are indexed in databases as BGSC (Bacillus Genetic Stock Center), so it is easy to find.   
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Auxotroph <i>B.sutbilis</i> strains already exist and are indexed in databases as BGSC (Bacillus Genetic Stock Center), therefore it is easy to find.   
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Revision as of 14:29, 12 October 2014